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We narrowed to 803 results for: TIM

Showing: 381 - 400 of 803 results

  1. Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections

    Type
    Blog Post
    Published
    March 10, 2016, 3:30 p.m.
    ...out in a myriad of journals every week, it can sometimes be difficult for great work to gain prominence.... Addgene's "How to cite this plasmid" section Anytime you use a plasmid from the repository, we request... to repeat the modification. This will save both time and money. A quick note -  While it is tempting ...

  2. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    Published
    Dec. 10, 2015, 3:30 p.m.
    ...accelerate your research. We know you don’t have time to be waiting on hold, let alone waiting multiple...support team available whenever you need them, but sometimes that’s not enough. With your busy schedule, you...features that allow users to help themselves, saving time and effort for everyone involved. We’re adding self...

  3. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    Published
    Aug. 13, 2014, 3:49 p.m.
    ...on molecular features found in nature, with the ultimate goal to improve the diagnosis of disease, and ...purchase and use the SpyLigase system for the first time? Howarth: The Proceedings of the National Academy...17] should cover it. Addgene: OK, thanks for your time, and thanks for depositing your plasmids with Addgene...

  4. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...a modification of interest. While the authors optimized the workflow for use in a fetal-lung derived organoid...are costly, non quantitative or require heavy optimization. Claudia Vickers’s lab has designed novel SL... genes with 10 gRNAs per gene. The guides are optimized to maximize on-target cleavage and minimize off-target...

  5. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    Published
    June 10, 2025, 1:15 p.m.
    ...19% integration in HEK293T cells (more than 500 times better than the precursor it was evolved from!) ...to specific mutations require individualized optimizations, safety testing, and approval. In contrast, ...higher efficiency, but evoCAST required less optimization for new targets and produced a higher-purity...

  6. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    Published
    April 25, 2024, 1:15 p.m.
    ...fresh insights and broaden your understanding, ultimately enhancing your project's potential. Widening ...mindful meditation, taking field trips, and spending time outdoors in nature or visiting museums can be excellent...ideas. It's beneficial to carry a notebook at all times (or use the notes app on your phone) to capture ...

  7. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...so that you can spend less time researching the basics and spend more time developing cleverly designed... Gateway, Gibson, etc). The cloning method is ultimately chosen based on the plasmid you want to clone...

  8. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    Published
    May 2, 2023, 1 p.m.
    ...methods missing something? Here we will review some time-tested gene identification systems that may have...expression levels (high/low) and patterns (tissue/timing) can be monitored under various conditions without...require the trapping of endogenous enhancers to stimulate the expression of the reporter. The system is ...

  9. Oh, The Places You Can Go: Careers in Science Communication - Product Development

    Type
    Blog Post
    Published
    Oct. 18, 2017, 1 p.m.
    ...helped grad students who were teaching for the first time. I walk[ed them] through their lecture, giving them...interests. “So for me, I researched G-proteins at the time. This program takes what you know about it [your... I was. It was really luck of right place, right time, and having a good interview experience.” Although...

  10. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...conditions, cells must be able to detect extracellular stimuli. One way they do so is through G protein-coupled...engineering and experimental refinement to determine the optimal localization of the Rluc8 insertion within the ...refinement, it was not possible to generate an optimal Rluc8-Gα construct for two of the 16 non-visual...

  11. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    Published
    Nov. 22, 2016, 3:30 p.m.
    ...more likely) impossible to find; therefore all the time spent collecting it will have been worthless. But...ASM CEO Stefano Bertuzzi welcoming us. Then it was time to work! Set up similarly to the online class, we...technique, then type up the materials and methods. Over time that portion of the paper will be generated. After...

  12. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ...Check out Joanne's Reddit AMA  Few of us spend much time thinking about conserving resources in the labs....wasteful packaging.  Turns out, one could make a lifetime supply for about $10 US dollars worth of glass... our protocol for making competent cells to save time and money. My other stingy science project started...

  13. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    Published
    Jan. 16, 2014, 2:56 p.m.
    ...whether an advisor will be a good mentor: Spend time with the potential advisor and don’t be shy about...lab, if possible. Attend a lab meeting. Spend some time hanging out with members of the lab. It is a warning...if the PI does not welcome you to visit and spend time with everyone. Additionally, if you are looking...

  14. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ...undergraduate degree (and I hope I’m not alone in this sentiment). Entering the final semester of a neuroscience...findings of brain science, my curiosity did not ultimately translate to a desire to do the research itself...percolate within a new intellectual environment. Over time, this interest came to incorporate other facets ...

  15. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ...communication research.   This summer, we’re making time to talk about my favorite thing - science communication...knowledge than average and are more likely to spend time engaging with science communication efforts.  Scientific...about science communication itself! See you next time!  Additional resources on the Addgene blog 10 ...

  16. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ...something Stegmeier has been interested in for a long time. “At an early age, I was fascinated by the impact...therapy.” He’s also worked on vaccines throughout that time. Now Vandenberghe is an Associate Professor at Mass... walls we’ve built create inefficiencies that ultimately make innovation and science slow,” Vandenberghe...

  17. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ...Science Magazine. Although we only walk one path at a time, the variety of paths in life is mind-boggling. ...aspects of bioscience into context, while at the same time working through the details of experimental design...discoveries into an entertaining podcast? Look at our multimedia producer and online editor. And, the copyeditors...

  18. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ...antibodies that have almost certainly already been optimized for your application. Depending on the tag, directly...of epitope tags exist – all with individual optimizations and specific monoclonal antibodies. Below we... acid tag (DYKDDDDK) that can be added multiple times (often 3X FLAG, but can be more!) to a protein’s...

  19. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...this; however, you should reduce your incubation time on ice to 5 minutes (incubating the full 30 minutes...group! You may want to include extra extension time after the last cycle of PCR to make sure that the... is not recommended that you exceed the 5 minute time limit (lower transformation efficiencies have been...

  20. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ...launched in the early spring of 2015 and since that time have received about 3 project proposals per month...making connections and finding mentors -- well, it's time you call upon these people for guidance.  Before... evolve and respond to feedback throughout its lifetime.  In this way, you will always improve and give...
Showing: 381 - 400 of 803 results