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We narrowed to 845 results for: CAL;

Showing: 571 - 600 of 845 results

  1. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    Published
    April 25, 2024, 1:15 p.m.
    ...let curiosity guide your intellectual journey. Practical approaches One approach to achieve this goal ...chemistry, even if your primary focus lies within a biological lab. Engaging in such seminars exposes you to...ourselves moments of release and mental freedom. Practical approaches Engaging in mindful meditation, taking...

  2. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ...American Cancer Society, or maybe you think about a local food pantry or community arts organization. Many...services we provide like quality control, curation, technical service and open-access educational resources....decisions at Addgene If you’re ever curious what specifically drives the decision making process at Addgene...

  3. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...you covered! His His tags, often called polyhistidine tags, typically include six consecutive His residues...(Fox and Waugh, 2002). The tag can also be proteolytically cleaved after purification, so as not to perturb...

  4. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    Published
    June 29, 2023, 7 p.m.
    ...required to complete a six month work experience called a co-op to receive a degree. Northeastern compiles... occasional TikTok. I was also responsible for typical entry level tasks such as stuffing new depositor...connections with coworkers seem, in retrospect, nonsensical. I have found the people at Addgene have become...

  5. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...wavelength of light. This response is immediate, on the scale of milliseconds, and can precisely link a neuron's...control over timing. Because chemogenetics relies on chemical activation and the diffusion of drugs throughout...require a permanent intracranial implant to be surgically installed to allow for light delivery. Chemogenetics...

  6. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    Published
    Nov. 29, 2016, 3:30 p.m.
    ...sometimes no culprit can be found…. In our lab we call this mysterious source of failure the lab gnome....There must be some way to use LEGOs, which are practically universally loved (except for those rare times...children who have no preconceived obstacles, optimistically grasp at limitless potential, and who sometimes...

  7. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ...with people, nurturing networks, as much as with technical knowledge. I leverage a breadth of knowledge to...I am constantly teaching – often explaining a technical piece of research to others outside that specialty...punctuation can be wildly funny as well as financially critical. Those who work on the publishing side of science...

  8. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...the less acknowledged perks of scientific and technical training is that these educational paths prepare...Foundation study showed that 57% of PhDs in US Biomedical workforce will NOT go into “traditional” academic...but there is more flexibility with the academic calendar Academic researchers must be extreme self-starters...

  9. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...system by using a stronger promoter and different biochemical secondary energy sources to increase protein ...DNA of interest, and cell extract.    Graphical Abstract: Schematic representation of cell-free...sgRNA pool can also target core operons in other pneumococcal strains. The BARBEKO sgRNA library uses CRISPR...

  11. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ... While there were software tools available to biomedical researchers manipulating DNA sequences on a daily...different microbial strains. It proved to be impractical to catalog every variant of a feature. Instead...that tolerates occasional mismatches or indels. Empirical tests indicated that a reasonable rule is to require...

  12. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ... to a method of physically joining multiple fragments of DNA to create a synthetically designed DNA sequence...one single-stranded oligo, instead of the more typical two oligos, is more cost effective and eliminates...

  13. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    Published
    July 27, 2017, 1:11 p.m.
    ...it’s now easy to monitor protein expression, localization, and protein-protein interactions. Beyond these...known to bind to the HMO of interest. Fatima specifically proposes using this sensor to detect HMOs found...Fatima's recent publication on her HMO work in Cell Chemical Biology! Additional Resources on the Addgene ...

  14. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ...Microbiology, Biochemistry & Molecular Biology, and Biochemical Research Methods. We also pulled the big interdisciplinary...the papers with plasmids deposited at Addgene – typically about four times as many as papers without plasmids... of publication. Table 1 provides an example calculation of the weights. Table 1: Journal Weighting Example...

  15. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    Published
    Aug. 22, 2023, 1 p.m.
    ...the host university does not hire or pay them (typically). A visiting scientist is distinct from equipment...the scientist to the new institute where their typical workdays will be carried out until the end of the...one) to communicate your plan as well. Making logistical and funding arrangements Visiting scientist positions...

  16. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ... and 15/17 morphologically aligned. Documentation of the network that initiates biological reprogramming...10.1016/j.cell.2022.11.026 Joung J. et al., Genome-scale CRISPR Cas-9 knockout and transcriptional activation...

  17. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...30667360; PMCID: PMC6377228. Hyperfolder YFP- a chemically stable tool for microscopy by: Susanna Stroik...many of these applications have lagged. Fixation, chemical staining, and light exposure can negatively affect...Find YFP plasmids here!   Campbell, B.C., et al. Chemically stable fluorescent proteins for advanced microscopy...

  18. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...particle to infect a cell, it has to come into physical contact with that cell. Since there is a limited... by an anterograde AAV was shown to drop off drastically with lower titers. As you can see in figure 2...AAV-mediated gene expression in a target cell. Practical considerations If you have high quality AAV, you...

  19. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    Published
    Nov. 7, 2019, 2:18 p.m.
    ...for actually making it happen would increase dramatically. I’m not sure what the best approach to doing...at The Scripps Research Institute in La Jolla, California. She joined Elsevier after her postdoctoral studies...primary research and review journals across life and physical science, and editorial contribution to the strategic...

  20. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    Published
    June 30, 2020, 1:15 p.m.
    ...virtual conferences may have apps or interactive calendars that you can use to help plan your conference ...one session to another,” Bachle says. “Now, the physical location didn’t matter. I didn’t have to consider...conference is over on the website or in the app. Technical difficulties? Attending a conference virtually...

  21. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ... reader accessing the information? This can be physical or digital. For instance, you would present information...most helpful to use a lot of jargon and have a technical, detailed approach. Other times, it is more helpful...thought it would be most helpful to highlight the technical details of collecting the purified AAV–that the...

  22. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    Published
    July 28, 2017, 1:30 p.m.
    ...study it. The process is fairly straightforward. Basically, you place your gene of interest in a plasmid ...genetic crosses with balancer chromosomes to make genetically stable fly lines. After a few weeks of waiting... aware of at least some of the possibilities. Basically, if you can devise an experimental assay that ...

  23. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ...North Carolina, Connie Cepko’s lab from Harvard Medical School, and Jonathan Ting’s lab from the Allen ...educational materials, and provides viral vector technical service (details about virus production protocols...word out about this new resource and providing fantastically useful educational content on viral vectors....

  24. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...chain constant regions result in several unique physical characteristics of these five isotypes, such as...their heavy chain constant domains. These are typically numbered, i.e. IgG1, IgG2, etc. Let’s dive in ...different constant domain gene. This process is called isotype switching and results in the B cell expressing...

  27. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ..., a powerful open-source search engine that can scale to search millions of web resources and handle millions..., partial words, phrases, partial phrases, or numerical identifiers. We also combine the different terms...PI labs and institutions. There are many more technical details that make our new search engine unique...

  28. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type
    Blog Post
    Published
    Feb. 19, 2019, 1:36 p.m.
    ...as help our students succeed whether that be academically or on the employment front. Paratore: I work...& Morning. Karen was a founding member of Mass Medical Angels, a venture mentor at MIT and served on the... the Greater Boston Food Bank and participates locally in Read To A Child's "Lunchtime Reading Program...

  29. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...Tim Miles, PhD, Director of the CLOVER Center at CalTech? If so, you may have submitted a question that ...Have you or others been able to successfully systemically transduce and then retransduce a model organism...as multiple sclerosis?  A: Basic science and preclinical researchers have used the flexibility of AAV ...

  30. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    Published
    June 30, 2016, 2:30 p.m.
    ...restriction endonucleases. A given prokaryote typically has genes encoding one or a few restriction modification...Well, even though Dam methylation sites are not specifically associated with any restriction modification... These specialized E. coli strains have been specifically engineered to be Dam and Dcm methylase-deficient...
Showing: 571 - 600 of 845 results