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  3. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...adapted from the 1979 Patterson Protocol. When published, the Patterson Protocol was shown to purify DNA...

  4. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ... of parameters influencing droplet rain in the Listeria monocytogenes prfA assay-reduction of ambiguous...

  8. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...restriction enzyme digest; however colony PCR can accomplish the same thing in less time and for less money...

  12. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...appear to be very effective probes for finely visualising sub-cellular structures or low-abundance proteins...

  16. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...mTurquoise2, mNeonGreen, and mScarlet-I for labeling a list of specific structures within cells. For example...

  17. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...2012) Bacterial plasmid partition machinery: a minimalist approach to survival. Current Opinion in Structural...

  18. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...these libraries as ingredients to a recipe. It’s unrealistic to plant, grow, and harvest grains every time...

  19. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...Creating a bioinformatics workflow in Nextflow Establishing a complete pipeline however, required more than...

  23. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...consider when designing a full gene. A non-exhaustive list includes: Codon abundance relative to cognate tRNA...

  25. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ... yourself for potential gRNA sites, or you can utilise several online tools that do it for you. One such...

  30. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...system, as is commonly used in western blotting or ELISAs.  A popular method for indirect labeling employs...
Showing: 571 - 600 of 611 results