We narrowed to 838 results for: IGH@

1. Streaking for Single Colonies: The Streak Plate Challenge

   Type

   Blog Post

   Published

   March 2, 2021, 2:15 p.m.

   ...fluorescent protein family that give off color in ambient light. Expressing these chromoproteins in E. coli would...

2. PEI Calculator for Planning AAV Packaging Transfections

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   Blog Post

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   Jan. 23, 2018, 2:04 p.m.

   ...plasmids at specific molar ratios. To get these ratios right, you need to do a few calculations. This calculator...

3. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

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   Blog Post

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   Nov. 15, 2016, 3:30 p.m.

   ...shape Addgene's future. Listen to this episode for insights into the process of getting an MD PhD, the inside...

4. Tips for Working in Industry & Success in Management: Interview with Crystal Shih

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   Blog Post

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   Oct. 5, 2017, 1:13 p.m.

   ... about Crystal’s exciting career path, to gain insight into industry research, and to get advice on how...

5. Depositor Week 2020 is Happening Now. Join us!

   Type

   Blog Post

   Published

   Nov. 16, 2020, 2:15 p.m.

   ...back on some fascinating Addgene history that you might not have known about. Here's the schedule for the...

6. New Viral Vectors - Spring 2025

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   Blog Post

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   April 29, 2025, 1:30 p.m.

   ...interneurons. Take a look to see if these new preps might enhance your research!   Table 1: New ready-to-use...

7. Interview with Bart Newland, Addgene’s Newest Board Member

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   Blog Post

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   Aug. 3, 2021, 1:15 p.m.

   ... school for microbiology, and I ended up going straight to law school. This was in the early 80s, and ...

8. Addgene’s Newest Way to Speed Your Research: The Cloning Grade DNA Service

   Type

   Blog Post

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   July 28, 2020, 1:15 p.m.

   ...open to industry scientists. This collection is highly diverse in terms of target organisms (from bacteria...

9. Editor's Choice, October 2016

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   Blog Post

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   Nov. 4, 2016, 2:30 p.m.

   ...for detecting genome edits, to discover why you might want to use Reddit to discuss your science and more...

10. Celebrating One Million Plasmids Shared!

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    Blog Post

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    Sept. 25, 2018, 2:39 p.m.

    ...plasmids shipped. Reaching one million plasmids shared might have you wondering how many plasmids leave Addgene...

11. Providing Plasmids to Researchers in Developing Countries: Addgene and Seeding Labs Team Up

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    Blog Post

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    April 5, 2016, 2:30 p.m.

    ...the labs they’re granted to, as well as shine a light on some of the great minds working in developing...

12. 2022 and 2023: Reflections and Looking Forward

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    Blog Post

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    Feb. 23, 2023, 2:15 p.m.

    ...community through sharing science and distributing high-quality materials and information. As a scientist...

13. Your Top Requested Plasmid in 2015!

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    Blog Post

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    Dec. 30, 2015, 3:30 p.m.

    ...lentiCRISPR v2 as the backbone for their many gRNAs. The high titer lentivirus production afforded by lentiCRISPR...

14. Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies

    Type

    Blog Post

    Published

    Feb. 26, 2019, 1:40 p.m.

    ... Find job listings at Addgene Get more career insight from Addgenies ...

15. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type

    Blog Post

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    Jan. 23, 2014, 3:23 p.m.

    ...One Pot, One Step, Precision Cloning Method with High Throughput Capability." PLoS ONE. 3(11): e3647 (...

16. Reflections and Looking Ahead

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    Blog Post

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    Feb. 25, 2025, 2:15 p.m.

    ...contributions to key research papers are already inspiring higher standards in antibody use (Ayoubi et al., 2024;...

17. Stepping into a Virtual Internship: My Experience at Addgene

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    Blog Post

    Published

    Aug. 13, 2020, 1:21 p.m.

    ...little more certain that I was being led down the right path. The frequent Zoom meetings helped me communicate...

18. Easing the Protein Purification Process with pCri

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    Blog Post

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    June 19, 2015, 3:08 p.m.

    ...expression system. If you find that your protein might not fold well, you should screen a few different...

19. Science as Art

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    Blog Post

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    March 2, 2015, 3:13 p.m.

    ...microscope, you too can become an artist. Scientists moonlighting as artists can compete in the Nikon Small World...

20. Tag Your Favorite Yeast Genes with Ease

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    Blog Post

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    Nov. 19, 2013, 2:37 p.m.

    ...looking at their performance in categories such as brightness, stability, and disruption of the tagged protein...

21. Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies

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    Blog Post

    Published

    Nov. 27, 2019, 2:03 p.m.

    ...be targeted with optical stimuli. The restricted light exposure to a specific area in combination with ...

22. Mapping the 4D nucleome with CRISPR/Cas9

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    Blog Post

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    Aug. 11, 2015, 2:30 p.m.

    ... Since the signal level provided by SunTag is so high, cells may be imaged under lower illumination settings...

23. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

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    Blog Post

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    June 29, 2023, 7 p.m.

    ...pinpoint exactly when I realized I had made the right decision to choose Addgene for my co-op, but I know...

24. Antibodies 101: Fc Effector Functions

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    Blog Post

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    May 18, 2023, 1:15 p.m.

    ...Antibody dependent cellular cytotoxicity (ADCC) is a slightly more direct method than opsonization. In ADCC,...

25. Cellosaurus: A Cell Line Information Database

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    Blog Post

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    Feb. 21, 2023, 2:15 p.m.

    ...recommended names and synonyms will all get you to the right entry.) You can look up the cell lines you are currently...

26. Fostering communication in remote collaborations

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    Blog Post

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    April 21, 2020, 1:14 p.m.

    ...document may sound like something out of your nightmares, shared ownership of materials produced from ...

27. Plasmids 101: What is a plasmid?

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    Blog Post

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    April 2, 2020, 6:17 p.m.

    ...troubleshooting tips to make plasmid usage as simple and straightforward as possible. If you have a question about a...

28. New CRISPR Web Resources and #12DaysofCRISPR Recap

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    Blog Post

    Published

    Dec. 12, 2017, 3:13 p.m.

    ...’s not our only job - we strive to also give you high-quality educational resources to help you do better...

29. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

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    Blog Post

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    Nov. 1, 2017, 2:04 p.m.

    ...faculty and introducing the kits to clients who might initially be doubtful of online science lab classes...

30. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type

    Blog Post

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    June 5, 2017, 3:19 p.m.

    ...productivity by providing world-wide access to curated, high-quality data and reagents Addgene makes it easy ...

31. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

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    Blog Post

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    Aug. 22, 2019, 12:43 p.m.

    ...consider. Antibiotic selection Selection is straightforward in cases where the inserted DNA includes an...

32. Choosing Your Perfect Empty Backbone

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    Blog Post

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    Aug. 19, 2014, 3:39 p.m.

    ...an opportune time or go all out with constitutive high level expression? Finally, you can add bells and...

33. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type

    Blog Post

    Published

    Aug. 18, 2023, 4:15 p.m.

    ...-enveloped virus (left) and an enveloped virus (right). Created using biorender.com   If you’re new to...

34. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

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    Blog Post

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    Sept. 14, 2021, 1:15 p.m.

    ...of troubleshooting failed experiments. Pick the right strain While common E. coli strains like DH5-alpha...

35. Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life

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    Blog Post

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    April 12, 2018, 1:02 p.m.

    ...fertilizer, seeds and a growth space with fluorescent lights. Other experiments require only seeds, petri dishes...

36. In Vivo Biotinylation of Bacterial Fusion Proteins

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    Blog Post

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    Jan. 25, 2018, 2:09 p.m.

    ...biotin-avidin binding could provide a true measurement of high affinity (i.e. sub-nanomolar) protein-ligand interactions...

37. Identifying Sequence Elements with SnapGene's Feature Database

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    Blog Post

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    Dec. 21, 2017, 2:06 p.m.

    ...elements Coding sequence features are relatively straightforward to define, but for control elements such as...

38. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

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    Blog Post

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    Dec. 13, 2018, 1:14 p.m.

    ...serotypes are equal; while some serotypes are typically high yielding, for others, such as AAV2, low yields are...

39. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

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    Blog Post

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    Nov. 8, 2018, 3:47 p.m.

    ... the same protein, their gene sequence may have slight or large variations depending on how closely related...

40. Plasmid Cloning by PCR

    Type

    Blog Post

    Published

    March 29, 2016, 2:30 p.m.

    ...amplify your insert DNA. It is important to use a high fidelity polymerase to minimize mutations. The fidelity...