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We narrowed to 889 results for: ANT

Showing: 381 - 400 of 889 results

  1. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    Published
    Aug. 22, 2023, 1 p.m.
    ...ideal for you. Here, we will chat about who might want to explore this role, how to seek out such an arrangement...them to a new institute and mentor that they may want to consider for future work placements. The chance...identified a gap or need in your research and decided you want to pursue a visiting position. What now? First, ... for the new placement. Make sure you read any relevant literature for the techniques you will be performing...these expenses, or you may need to seek out travel grants available from third parties or your home institute...generate data that will contribute to a publication? A grant? A patent? It is never too early to discuss authorship...feelers, apply to programs, and apply for travel grants. Good luck and happy travels! Resources Resources...

  2. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ... within tens of minutes (Cabantous et al., 2013). The Shu Lab took advantage of this and came up with ...signals are often difficult to detect, and thus quantify, due to small fluorescence changes of the donor...protease activity (To et al., 2016). However, the lab wanted to increase the fluorescence range to better study...on protease activation. Normally, β10-11 are antiparallel. To prevent binding, the β strands in FlipGFP...sequence, β10 and β11 can rearrange to their normal antiparallel position and fluoresce. FlipCherry: Red fluorescence...FlipGFP and FlipCherry at Addgene. References Cabantous, Stéphanie, et al. "A new protein-protein interaction...

  3. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...CRISPR/Cas9 could be used to edit several autosomal-dominant mutations that have been connected to the early...Chinese Academy of Sciences found that mice with a dominant mutation in a gene that causes cataracts could... Cas9 mRNA and a single-guide RNA targeting the mutant allele (7). An independent team led by Hans Clevers... chose to explore its potential in mice with a dominant cataract disorder caused by a single copy of a...of the cystic fibrosis report. “This is a big advantage over induced pluripotent stem cells, which need...and many others. “Those off-target effects are important,” she said. “We also really need to get a handle...optimization. In addition, the current work in developing anti-CRISPR proteins aid the limitation of these mutations...

  4. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...studied for many decades, and they were known to be resistant to detergents and degradation under a variety ...different pH values. Using a screen that takes advantage of the fact that coiled R-bodies sediment in certain...see figure to the right), we identified R-body mutants that extend at both lower and higher pH. We initially...initially used this screen to find R body mutants that couldn’t extend at a normal pH and later realized...Plasmids expressing expressing both wild type and mutant R bodies can be found at Addgene here. What can...F. R. Organization and expression of genetic determinants for synthesis and assembly of type 51 R bodies...trait: Absence of toxicity of R-body producing recombinant E. coli on paramecia. European Journal of Protistology...

  5. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...something like an antibody, enzyme, DNA, etc. The receptors job is to generate a quantitative signal upon recognizing...sense’ biological compounds and reactions; you can quantify them with biosensors! In this blog we will review...biological system. Basically, biosensors provide quantifiable readouts for levels of different chemicals, ...biosensor is the signal transducer which will quantitatively give a readout for whatever the bio-recognition...already engineered into viral vectors, so if you want to use them, you’ll need to use the vectors (or ...good thing, though! Viral delivery has several advantages over other mechanisms. For one, many viral platforms...

  6. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...site-specifically to alter its function, mimic a biologically relevant modification, or add in fluorescent or chemically... technologies have idiosyncrasies presents a significant barrier for those wishing to use it as a tool... or easily available, so you may find yourself wanting to use a protocol that you’ll have to rework to... They provide resources, knowledge, and most importantly, validated protocols (using readily available...their first protocol is available now. It is for recombinant expression of phosphoserine-including proteins... protocols come out! References Noren, C. J., Anthony-Cahill, S. J., Griffith, M. C., & Schultz, P. G...Site-specific Incorporation of Phosphoserine into Recombinant Proteins in Escherichia coli. 12(21). https:/...

  7. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ... for analyzing time-lapse images, quantitatively evaluating antibody staining, or counting cells are now...biology, innovative new imaging approaches are constantly being developed which combine computational processing... process framework FIJI are just three of the constantly growing number of "community-driven" software... fix security-critical bugs, and contribute to constant updates of the software. In hardware, this process...can literally put their work on the shoulders of giants. The still relatively young movement of sharing...Chew Chai, Manu Prakash, "Squid: Simplifying Quantitative Imaging Platform Development and Deployment....

  8. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ...cellular pathway of interest conveys no significant survival advantage, such as a non-essential mechanism ...aging. For both a screen and a selection, it is important to clearly define what type of factor you are ...conditions. For screens, there is no obligatory growth advantage – that is, you are not specifically looking at...throughput? Will it be sensitive enough? No one wants to spend time screening hits that aren’t “real” ...lowering the culture temperature to exacerbate a mutant phenotype, very similar to enhancer/suppressor ...condition that can apply enough pressure to select for variants of interest, though. A selection can take the ...

  9. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    Published
    Sept. 15, 2020, 1:15 p.m.
    ...have gained or lost a specific gene survive (ex: antibiotic resistance marker). In a previous blog post, ... your plasmid backbone, as it often relies on antibiotic resistance. But what about identifying clones...𝛽-galactosidase activity. These cells, presumably the cell you want, cannot break down X-gal and are white. Cells that...to be strategic about your enzyme choices. You’ll want to pick enzymes that will give you different sized...to amplify anything. Like any experiment, you’ll want the proper positive and negative controls for your...provide all of the information you need, so you'll want to design a custom primer. Because Sanger sequencing...wouldn't be reached by sequencing from either terminus. Want to know more? Visit this blog post for tips for ...

  10. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    Published
    July 10, 2014, 7:17 p.m.
    ...synthetic biology to target and light-up bacteria unwanted in food, healthcare settings, etc. What project...engineer bacteriophages and use those to detect unwanted bacteria. How did graduate school help prepare...of technologies, effectively communicating the advantages embodied, and driving the enterprise value of...it by reading a broad variety of materials and constantly practicing. “The expenditure of effort in preparation.... Second, "an entrepreneur must be OK with the constant fear of failure at all stages in the startup"....potential conflicts that waste money and time. Most importantly, “the company does not become real until someone...Before seeking funding, ask yourself if you really want to do this.” Biotech startups are only successful...

  11. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...genetics was booming, SciFi saw the rise of literary giants such as Isaac Asimov, Arthur C. Clarke, James Blish...editing technology led to the development of many important medical and agricultural products. But it also... that revolves around synthetic humans called replicants which are genetically engineered by a powerful...recognition sites had to be designed each time we wanted to target new genomic sites. The discovery of the... (Langdon). The plot involves Langdon chasing an anti-villain geneticist named Zobrist who designs a virus...plot of the movie involves The Rock and his super giant albino gorilla fighting off a monstrous wolf and...SciFi is one of the most imaginative and most important genres of literary fiction. It has always served...

  12. Finding Your Science Policy Path

    Type
    Blog Post
    Published
    Sept. 22, 2020, 1:15 p.m.
    ...are ways you can hone important skills for the profession. One of the most important skill sets for a science... of the who, what, where, when, why and most importantly, how, of a science policy career.  Who does science...approval for a new product or a well-endowed philanthropy who can shift the funding landscape to focus...increased science funding and other issues. Whether you want to pursue science policy as a full-time career or...science policy? I would argue that this is the most important question that you can explore. The answer will...advice for you and your next steps. This advice is important, but must be taken through your own personal lens...you determine what parts of your current job you want to keep or change and can inform your move into ...

  13. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...optogenetics, and chemogenetics experiments. Recombinant AAVs (rAAVs) The primary application of AAV is...the deliverable DNA) are removed, resulting in recombinant AAVs (rAAVs) with maximum “trunk” space for genetic...specific cells and/or tissues is one of its biggest advantages. Specificity can be achieved using specialized...specificity; this is particularly helpful when wanting to target specific neuron subtypes, since AAVs ...and our AAV data hub to see if others have used relevant viruses for your system. This post was contributed...and Richard J. Samulski. "Self-complementary recombinant adeno-associated virus (scAAV) vectors promote..."Development and optimization of a real-time quantitative PCR-based method for the titration of AAV-2 ...

  14. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...As a scientist, perhaps the most important part of your research is communicating the results of the ...the work. Yes, those control experiments are important — but without letting other scientists know the results...conference poster or talk, or submitting patent or grant applications; to informal settings like lab meetings...amounts of access or feedback. One of the most significant shifts in scientific communication is the rising... of listing “Submitted” articles in their CV or grant applications. A number of organizations (such as... or invite attendees to read and comment online! Want the general public (say, policymakers or community...research materials available to the community significantly sooner. Plus, authors can include the Addgene...

  15. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...White and Anthony Collmann of White Consulting frequently do sessions by Skype or phone. Samantha Sutton ... goals are for the coaching relationship. Do you want to change jobs? Grow your professional presence?... consultation meeting or call for free.  Take advantage of this to get the information you need to choose...a scientist from academia to industry, you might want to consult the Cheeky Scientist. Their motto is ...coaches I have mentioned. I have seen a lot of fantastic online content for PhDs and postdocs posted by...bottom line – there are lots of people out there who want to help. Don’t be afraid to ask and to make an investment...

  16. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...modular cloning toolkit for genome editing in plants. BMC Plant Biol 20, 179 (2020). https://doi.org/10.1186...libraries depend on many gRNAs delivered with a relevant Cas nuclease. MoClo can be used to assemble multiplexed...are not available to those working with yeast or plants. Using MoClo, or better yet, a pre-made MoClo kit...achieve a desired effect. This is commonly used in plant biology to achieve traits such as disease resistance...blog on choosing a MoClo strategy and kit if you want to learn more about this cloning technique! Many...Cloning Guide EcoFlex MoClo Kit CIDAR MoClo Kit MoClo Plant Parts Kit Resources on the Addgene blog High Complexity...

  17. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...equipped using fast-acting optogenetic tools. The advantages provided by chemogenetics are instead that it...Targeted and specific delivery becomes especially important for studies in neuroscience where specific tissues...neuron need to be targeted. Targeting is also important in the development of therapies when expression...Picking Ligands Picking the right ligand is also important for building a chemogenetic system. Your experiment...cellular response you aim to manipulate. It is important to consider that the same receptor can elicit ...drug that only binds to one receptor, so it is important to fully characterize the compound, or compounds...will promote hyperpolarization. PSAM ligands The advantage of PSAMs lies in their ability to be specifically...

  18. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...process involves a significant amount of QC. First, we perform Picogreen quantification on each and every...high-quality isolated DNA samples of sufficient quantity for sequencing. This process is completed in a... preparation process and includes Picogreen quantification of various intermediate steps, a sizing electrophoretic...electrophoretic gel, and qPCR quantification of the final libraries. A broader view of the whole plasmid... Analysis Platform to Assess the Identity of Recombinant Adeno-Associated Viral Preparations from Viral...

  19. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ...machine or robot out there that does exactly what you want plus way more than you thought it could ever do!... taking a lot of time. Enter the Biomek FXP! We wanted a machine that would improve our transformation...purification We're developing ways to prep large quantities of DNA because some countries just can’t receive...fills all those vials? Well, it used to be a lab assistant, but with increased plasmid demand it was too ...fill 400 vials in 35 minutes compared to a lab assisstant filling about 200 vials in 45 minutes by hand...have used in the lab. It’s especially useful if you want to avoid sore fingers. There are other process ...about more lab updates as we develop them. In the meantime, if you have any great lab automation tips and...

  20. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...selectable with the antibiotic whose resistance gene is encoded in the viral vector. This antibiotic selection ... 320,000 viruses can infect mammals. Even more abundant are the Earth’s estimated 1031 bacteriophages ...that infect bacteria), many of which are doing important work in our microbiomes. Given that viruses are...tips for your virus experiments. References 1. Anthony, Simon J., et al. "A strategy to estimate unknown...hepatitis B virus DNA and hepatitis C virus RNA quantification as measured with branched-DNA technology." ...PMC84922. 5. Ugai, Hideyo, et al. "Stability of a recombinant adenoviral vector: optimization of conditions...
Showing: 381 - 400 of 889 results