We narrowed to 397 results for: ARA-2

...rate of 10-55% - the PASTE system.     Fig. 2: Schematic of programmable gene insertion with PASTE...and Discovery As part of their quest to find, characterize, and develop new CRISPR systems and technologies...

...two visible blue or magenta chromoproteins (Figure 2) (Liljeruhm et al., 2018). This system is a nice confirmation...tested their expression under both rhamnose- and arabinose-inducible promoters. Tight control of toxin expression...escape). The rhamnose induction system is well-characterized and quite tight (Giacalone et al., 2006). The...though Khetrapal et al. also demonstrated that the arabinose promoter worked too. Rhamnose induction relies...References Espah Borujeni, Amin, Anirudh S. Channarasappa, and Howard M. Salis. "Translation rate is controlled...

... iRFP713, % Oligomeric state Photo-stabilityc, t1/2, s pKa Brightness in HeLa cells vs. iRFP713, % d ...and multicolor NIR imaging.  Small miRFPnano A separate class of NIR FPs, named miRFPnanos, were derived...developed based on mIFP6. Learn more about the characteristics, performance, and applications of near-infrared... found to be a dimer3,15. ND-not determined in parallel assays with other FPs.   We hope that this post...

...can still record the signal separately for each target.   Figure 2: Examples of simultaneous multiplex...or formalin-fixed, paraffin-embedded tissue sections, methanol- or paraformaldehyde-fixed cell samples...samples, or other preparations. Sequential IF For a sequential IF experiment (Figure 1A), you’ll block, add...different color fluorophore that you can image separately on your microscope. Abcam's sample protocol is...different antibodies, blocking, washing, and sample preparation steps, in order to ensure your antibodies are...Ilangovan, V., Lo, H., Olson, M., Mohamed, S. Y., Sarabipour, S., Varma, A., Walavalkar, K., Wissink, E. M...

...variety of environmental conditions, and double every 2 hours. Yeast are also the first eukaryotic genome ...as Parkinson’s disease (PD). PD is primarily characterized by protein misfolding that leads to a build-...23594743. PubMed Central PMCID: PMC3703927. Jennings, Barbara H. "Drosophila–a versatile model in biology & medicine...

...become a valuable asset yourself.     Fig 2: A diagram of the Thomas-Kilmann Conflict Model, courtesy...favorite books, Michael Ende’s Momo, the title character endears herself to the nearby village (and to ...or how completely different teams are tackling comparable conflicts. Is this level of domain knowledge ...

...There are approximately 2-4 million proteins per cubic micron in bacteria, yeast, and mammalian cells...proteins can then be purified using streptavidin and separated via flow cytometry.  Find plasmids for EXCELL ...

... Watch Joanne give her "Not" Networking 101 Talk 2. Tailor every talk to the occasion Don’t just plan...come up with clear and concise answers. This preparation will help you avoid giving only yes or no answers...

...PubMed PMID: 3459152. PubMed Central PMCID: PMC323600. 2. Chester, Nicholas, and Daniel R. Marshak. "Dimethyl...small difference in size, the fragments should be separated on a high percentage agarose gel (~3%). Note that...

... from damage (Abe & Lim, 2024).    Figure 2: A) When proteins (blue) interact with the air-water...RvLEAMshort (1:6 molar ratio) with 10 minutes of glutaraldehyde crosslinking: representative micrograph (C)...allowed the authors to determine structures at comparable or higher resolution than using more complex ...buffer additive MgCl2 and the crosslinking agent glutaraldehyde. Adding RvLEAMshort to the sample before plunge...

...biology 14.6 (2004): 757-764. PubMed PMID: 15582400. 2. Tomkinson, Alan E., et al. "DNA ligases: structure...the ligation junction were represented. Sixteen separate pools were prepared, each with a different complement...applications such as routine cloning or DNA library preparation. However, when a method relies on accurate ligation... 19329793. PubMed Central PMCID: PMC2719376. 5. Barany, Francis. "Genetic disease detection and DNA amplification...PMID: 1986365. PubMed Central PMCID: PMC50775. 6. Barany, Francis, and David H. Gelfand. "Cloning, overexpression...PMCID: PMC208194. 8. Luo, Jianying, and Francis Barany. "Identification of essential residues in Thermus...76.2 (1989): 245-254. PubMed PMID: 2753355. 12. Barany, Francis. "The ligase chain reaction in a PCR world...

...experience in cancer research After graduating with a 2:1 BSc in Molecular Biology (roughly a B average in...really passionate about during my degree. I loved parasitology. However, when I enquired about roles in this...end of my three months, I fully understood why parasitology roles in the field require experience. Working...beginning of 2010, I explored options for going into parasitology but another major factor held me back; I do ...

...Nutr, 129 (2009a): 477–484. PubMed PMID: 10064313. 2. Chapman-Smith, A., & Cronan, J. E. “The enzymatic...Forster, A. C., Skingle, D. C., & Symons, R. H. “Preparation and uses of photobiotin.” Methods in Enzymology...

...effective recovery of knock-ins, but did result in 1-2 kilobases of additional sequence being introduced....brilliantly and irreverently illustrated in the parable of the Geneticist vs the Biochemist, we geneticists...selection allowed me to optimize a range of editing parameters: oligo repair templates with homology arms of... template design The Meyer lab meticulously characterized several aspects of editing. First, they devised...doi.org/10.17912/DDVH-BG64 Tzur YB, Friedland AE, Nadarajan S, Church GM, Calarco JA, Colaiácovo MP (2013)...

...Single-chain variable fragment (ScFv) Figure 2: Comparison between the IgG antibody and scFv. ...the surface of extracellular pathogens such as parasites or microbes, or to proteins expressed on the surface...containing a mixture of proteins. The proteins are separated based on molecular weight by SDS-PAGE, and are...

...ask new and interesting questions in your own lab. 2. Seek multiple mentors during your postdoc At the ...corresponding author publications (published, not in preparation) A history of successful funding (fellowship ...

...module_attribute "schema_version" is_json="true" %}{% raw %}2{% endraw %}{% end_module_attribute %}{% module_attribute...technology, and the user may need to optimize numerous parameters to generate the desired edit. The wrench: Gene...