...350,000 individual plasmids to 5,000 different research institutions. This has given us a unique window...," Andrew Fire's set of vectors for C. elegans research was our most requested item of 2005. shRNA - In...Thank you for inspiring us with your breakthrough research and your commitment to scientific sharing. In ...

...A flexible and modular optogenetics tool The researchers also showed just how flexible their LITE system...conventional optogenetic tools." In total, the researchers applied the LITE system to 28 different genes... uses.” Konermann said she is already aware of research groups making use of the new optogenetic tools...

...public access to the results of federally-funded research. 2)      In June, the White House honored 13 “...enterprise lending their support to open scientific research data around the world. 4)      The Annals of Internal...more than 10,500 microarray studies found that researchers who made their data available to others got a...

...give honest, constructive feedback, set tangible research and career goals, and celebrate accomplishments...and Planning Worksheet: Lists accomplishments, research/professional goals and feedback. Calendar: ...principles behind this technique are beneficial for researchers at all stages. Rather than succumbing to academic...

...genomic damage, specifically double strand breaks. Researchers have long taken advantage of this natural process...genome tagging systems enabled you to advance your research? Do you have a favorite tagging system that isn't...Pringle JR. Yeast. 1998 Jul;14(10):953-61. Note: Marcy Patrick contributed to the writing of this article...

...expansion will give researchers increased flexibility when planning their research and expanded choice...and ordering. This split allows us to provide researchers with increased choice and flexibility; while ...

...DNA double-strand breaks (DSBs) preferred by researchers. Compared to non-homologous end joining (NHEJ... efficiency Since the development of BE3, many research groups have made improvements to base editors,...indel frequency. Koblan et al., 2018 BE4max, ArcBe4max, ABEmax   Improved nuclear localization and expression...-0491-6 Grünewald, J., Zhou, R., Lareau, C. A., Garcia, S. P., Iyer, S., Miller, B. R., Langner, L. M....

... and disease. To observe bacteria in action, researchers have developed in vivo bacterial reporters that... by water-dwelling photosynthetic bacteria and archaea to regulate their buoyancy. The Shapiro lab recently...Want to start using acoustic reporters for your research? arg1 and arg2 plasmids are available from Addgene...

...in the educational sphere, it can range from researching an area, to writing an introduction that gives...mitosis and meiosis in biology. Lots of it is researching the different aspects of the subject you're writing...writer at the non-profit Fred Hutchinson Cancer Research Center. And make sure to subscribe to the Career...

... portion of lab waste. Despite the fact that researchers represent a minority of the world’s population...environmental impact, but we can all do more to make research more environmentally friendly. Adopt one or all...Subscribe to the Addgene blog Find lab tips for your research Read another blog post about conservation in the...

...families have been characterized. To date, 83% of archaeal and 45% of bacterial genomes utilize this system...translative technologies. Addgene has empowered researchers to harness previous experimental successes and...CRISPR Guide or find CRISPR/Cas plasmids for your research. Do you have a cool idea or application that involves...

...Arnold  Listen to the MarathonRT podcast segment Researchers from Dr. Anna Pyle’s lab at Yale University recently...transport at the cell surface Article contributed by Marcy Patrick  Listen to the nanobody toolkit podcast...them be analyzed by mass spectrometry allowing a researcher to specifically identify proteins that are enriched...

... were software tools available to biomedical researchers manipulating DNA sequences on a daily basis, ...increasing popularity of gene synthesis, many researchers now use codon-optimized versions of common coding... so our detection system was enhanced to allow searches for a perfect protein sequence match even when...

...stories all the time. They provide accounts of their research struggles in papers. They recount accomplishments...expanding the impact and appreciation for their research. Long story short, telling a good story is something.... And you won’t need to dive as deep into your research at an outreach event as you might in a scientific...

...are fewer outside influences on your topics of research or course of action One can focus on science for...flexibility with the academic calendar Academic researchers must be extreme self-starters and especially ...structures are common Small integrated teams, little hierarchy Real diversity and acceptance can be an issue...

...over to work on career skills development. Back in March, we posted these 25 great tips for scientists working... compiled lists of grants: Science: Where to Search for Funding The Ohio State University; Graduate... Database: 100 Places to Find Funding For Your Research University of Florida: Training Grants and Other...

...Hot Spring at YNP, and since then, many researchers have searched for novel organisms and enzymes from these...our CRISPR guide Find CRISPR plasmids for your research Find links to CRISPR references ...

...Ford  Listen to the riboglow podcast segment Researchers from the Palmer lab at the University of Colorado...cleavage activity Article contributed by Angela Kaczmarczyk  Listen to the base editing podcast segment The...reporter plasmid! Martin et al., Nucleic Acids Research 2018 PubMed: PMID 29746667. ...

...requirements. To solve this problem, Gao et al. searched for Argonaute orthologs similar to TtAgo and PfAgo...efficiency. Higher sensitivity to off-targets More research is needed to compare CRISPR and NgAgo, but it ... 3. Swarts, Daan C., et al. “Argonaute of the archaeon Pyrococcus furiosus is a DNA-guided nuclease that...

... red light instead of blue, which will allow researchers to independently control the activity of two .... Understanding the brain through optogenetics research Addgene: What are some of the big problems that...Optogenetics Plasmids? Plasmids for Optogenetics Research (Addgene) Plasmids from Ed Boyden's lab Sign ...