We narrowed to 516 results for: ATO

1. Multiplex Genome Editing with CRISPR-Cpf1

   Type

   Blog Post

   Published

   May 9, 2017, 2:12 p.m.

   ...multiplexing vectors are larger because they require regulatory sequences to allow for expression of multiple...

2. Top Requested Lentivirus and AAV of 2016

   Type

   Blog Post

   Published

   Jan. 6, 2017, 3:56 p.m.

   ..., Koda S, Ye C, Rogan SC, Adams AC, Cusher DS, Maratos-Flier E, Roth BL, Lowell BB. Rapid, reversible ...

3. Communicating Your Science With Help From ComSciCon

   Type

   Blog Post

   Published

   July 19, 2018, 1:12 p.m.

   ...in all STEM fields become excellent science communicators. To empower graduate students to become ambassadors...

4. Why and how to start studying chromatin (with Addgene's help)

   Type

   Blog Post

   Published

   June 12, 2018, 11:40 a.m.

   ...Chromosome Organization. These aren’t the only investigators in the chromatin field who have deposited with...

5. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

   Type

   Blog Post

   Published

   June 6, 2019, 12:41 p.m.

   ...microRNAs (miRNAs) are recognized as important regulators of post-transcriptional gene expression across...

6. Plasmids 101: Screens vs. Selections

   Type

   Blog Post

   Published

   Nov. 10, 2022, 2:15 p.m.

   ...selected growth conditions. For screens, there is no obligatory growth advantage – that is, you are not specifically...

7. Building and Validating Recombinant Antibodies: A Case Study

   Type

   Blog Post

   Published

   April 5, 2022, 1:20 p.m.

   ...secondary antibodies are commonly used in research laboratories to detect the different subclasses, but there...

8. Antibodies 101: Monoclonal Antibodies

   Type

   Blog Post

   Published

   June 29, 2021, 1:15 p.m.

   ...the antigen-binding sites of the antibody - the paratopes - may change and impact the specificity and avidity...

9. From Lab Bench to Leadership: Transitioning into Management as a PhD

   Type

   Blog Post

   Published

   Jan. 21, 2025, 2:15 p.m.

   ...and leveraging tools like the Myers-Briggs Type Indicator or DISC assessments can help you navigate team...

10. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type

    Blog Post

    Published

    Sept. 26, 2024, 1:15 p.m.

    ..., the stem-loop structure of transcriptional terminators. Another potential issue is sequence similarity...

11. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type

    Blog Post

    Published

    Aug. 6, 2024, 1:15 p.m.

    ...Woodchuck hepatitis virus post‐transcriptional regulatory element; sequence that stimulates the expression...

12. Sharing is Caring: But How to Distribute Open Hardware?

    Type

    Blog Post

    Published

    Feb. 28, 2023, 2:15 p.m.

    ... in science brings stronger networking across laboratory and disciplinary boundaries and can allow for...

13. Behind the scenes: Addgene’s new search engine and more

    Type

    Blog Post

    Published

    Dec. 12, 2019, 2:22 p.m.

    ...of other websites, we built our new search engine atop Elasticsearch, a powerful open-source search engine...

14. How to Make Friends and Meet People at a Scientific Conference

    Type

    Blog Post

    Published

    Aug. 7, 2014, 1:58 p.m.

    ...You never know when you will meet a potential collaborator Some of these people you meet will review your...

15. Dos & Don'ts When Publishing a Scientific Manuscript

    Type

    Blog Post

    Published

    May 22, 2014, 4:02 p.m.

    ...communities. The ARRIVE guidelines for the reporting of laboratory animals during in vivo research and the CARE ...

16. Human Germline Editing Using CRISPR

    Type

    Blog Post

    Published

    Aug. 10, 2017, 2:19 p.m.

    ...proceed with caution and with proper oversight from regulatory and ethics committees comprised of individuals...

17. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type

    Blog Post

    Published

    July 25, 2017, 1:20 p.m.

    ...endogenous ERK, and its localization serves as an indicator of MEK1 activity. When MEK1 is active, it phosphorylates...

18. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type

    Blog Post

    Published

    Sept. 4, 2018, 12:53 p.m.

    ...of IPTG. Because RepA is a plasmid replication initiator protein, they could alter copy number of the plasmid...

19. RaPID Detection of RNA-protein Interactions

    Type

    Blog Post

    Published

    Aug. 16, 2018, 12:40 p.m.

    ...one to study RNAs expressed from their native regulatory elements. Controls for RaPID experiments RaPID...

20. dTAG - You're it!

    Type

    Blog Post

    Published

    June 21, 2018, 2:06 p.m.

    ...dTAG-47 Weintraub et al. YY1 Is a Structural Regulator of Enhancer-Promoter Loops. Cell. December 14 ...