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We narrowed to 847 results for: CAL;

Showing: 381 - 400 of 847 results

  1. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ...hereditary determinant”. His proposal, however, was basically ignored. A separate term, “episome”, defined as... plasmids have greatly impacted many areas of biological research and have been key in advancing our knowledge...Joshua. "Cell Genetics and Hereditary Symbiosis" Physiological Reviews 32.4 (1952) 403-430. Pubmed PMID: 13003535...PMC2134509. Cohen, Stanley N. et al. “Construction of Biologically Functional Bacterial Plasmids In Vitro.” Proceedings...Nomenclature for Bacterial Plasmids: A Proposal.”Bacteriological Reviews 40.1 (1976): 168–189. PubMed PMID: ...

  2. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ...Harvard Medical School and runs everything science (CSO). Clinton is a cost analyst at Dow Chemical (a Fortune...a company in the health sciences without those critical three letters after your name (P-H-D).  Finally...two projects, including one led by a talented biomedical engineering team from MIT.   I would like to ...connections and finding mentors -- well, it's time you call upon these people for guidance.  Before even beginning...

  3. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...has a maximum of one gene knocked out. In this genetically heterogeneous population, cells behaving differently...knockout mice for (all of the genes involved as I recall). The lab then used these knockout mice to show...cells we study are migratory, and their exact localization is important for their function. Their migration... you could majorly accelerate progression into clinical trials. Secondary screens  If you have generated...genes you’d like to test for phenotypic effects. Typically the phenotype would be studied in vitro, but there...The different labs use different algorithms to calculate which would be the best gRNAs. Interestingly, ...

  4. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    Published
    July 21, 2020, 1:15 p.m.
    ...might not have to do the calculation yourself as many fluorometers will calculate your sample concentration...than 1.5. Then, using the A260 reading, you can calculate the DNA concentration. Generally, A260 of 1.0 ... start by pouring your gel containing a DNA intercalating dye (ex: ethidium bromide) and choosing a DNA...quantified over time using a fluorescent dye that intercalates into the DNA such that smaller fragments are...

  5. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...these proteins are FDA-approved or in clinical trials or preclinical studies already. We are following up...expand to other cell types, including more physiologically-relevant systems, to catch all the important... Nsp3 and Nsp16, and includes an additional catalytically dead version of the viral protease, Nsp5. Our...Melanie Brewer (right) from the University of California, San Francisco. Manon Eckhardt and Melanie Brewer...

  6. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    Published
    Jan. 12, 2021, 2:15 p.m.
    ...understood what I was going through. (Parenthetically, when I called the lab to ask my benchmate to take...within a few days or a week) so they can easily recall the encounter. As a side note, my guess is that...; focus on the nice people who do respond. Don’t call or email multiple times if you don’t get a response...years ago using a now defunct (but fun) plug-in called Maps.  Figure 1: See a starfish? This is...

  7. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

    Type
    Blog Post
    Published
    Nov. 20, 2018, 8:17 p.m.
    ...to identify and reference documents, files, or physical reagents. What are persistent identifiers? Perhaps...persistent and exist online indefinitely. PIDs historically referred to objects that exist online, but this...approach has been adapted for the identification of physical materials that we use in our labs, including plasmids...Bourges for editing this post and sharing her technical expertise in persistent identifiers. Additional...Identification Initiative Compact identifiers for biomedical data How Addgene works to enhance scientific ...

  8. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...several treatments are currently being tested in clinical trials. It is speeding the process of the discovery...saw these two papers,” said the University of California Berkeley’s Jennifer Doudna. “They are the first...continue to be a primary preoccupation of her California lab and many others. “Those off-target effects...cleavage sites are repaired.” Those insights will be critical for scientists’ ability to influence editing efficiency...Application in Genetic Diseases: A Review.” Current pharmaceutical biotechnology. https://doi.org/10.2174/1389201021666200621161610...

  9. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ...both perform an optimization-scale infection and at least one screening-scale experiment achieving the gRNA...Chavez, researcher at the Wyss Institute for Biologically Inspired Engineering and member of the Addgene... studying. You need to make sure your assay is scalable and robust. What you don't need to do is spend...particular, the Church lab at the Wyss Institute for Biologically Inspired Engineering and the Zhang and Doench...

  10. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...four proteins Oct4, Sox2, Klf4, and cMyc (the so called OSKM factors). As the Yamanaka lab's primary Addgene...field has exploded so quickly, citing the first clinical trial with iPSCs with his collaborator Takahashi...picture perspective: Dr. Yamanaka talked about his clinical trial patients with advanced macular degeneration...Eggan presented work that is leading to promising clinical trials for ALS.   I have an uncle with Parkinson's...the first stem cell line that could be used in clinical trials. The quality control process alone takes...

  11. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...plasmid with the delivered cargo (often a gene) is called the transfer plasmid while the other plasmids with... with packaging or replication components are called helper plasmids. Below, we’ll breakdown the vector...viral experiments is to deliver a genetic cargo, typically an expression construct, to cells/organisms. This...yield dsDNA. Unlike LTRs, ITRs are perfectly symmetrical to allow for this pairing to occur. Like LTRs...gene, making it replication deficient. This is typically supplied by the production cell line (HEK-293)...

  12. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ...robust, with an eNL-based calcium sensor displaying a 500% signal change upon calcium binding. Suzuki et al...around these issues, but this solution wasn’t practical due to the low intensity of luminescent proteins... to previous publications. They also developed calcium, cAMP, and ATP sensors based on Nano-lantern, showing...not alter cellular physiology in your system. Optical sectioning cannot be conducted with luminescence...

  13. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    Published
    May 3, 2018, 1:48 p.m.
    ...nearly 50%, showing proof-of-concept for pre-clinical and clinical applications of dCasRx. The identification... with CasRx and AdmCas13d fused to a nuclear localization signal (NLS). In a HEK293 mCherry reporter assay...target essentially any RNA in a cell. Since catalytically dead dCasRx maintains its RNA-binding properties...enhancers. After AAV-mediated delivery to iPS-derived cortical neurons, dCasRx-mediated exon skipping improved...

  14. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ...made of chemicals, which have an unfortunate tendency to react with other chemicals and biological structures...antibody out on the bench overnight. Do not panic! Typically, antibodies are okay if they’re left out overnight... fluorophores, which work not by reacting to a chemical, but by emitting a specific wavelength of light...

  15. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...Bryan Roth’s lab created an open source resource called PRESTO-TANGO (parallel receptorome expression and...activated downstream of GPCRs. This collection, called TRUPATH, allows researchers to identify specific...neurons and specific behaviors. Chemogenetics uses genetically engineered receptors to interact with small molecules... to optogenetics but instead of light, these genetically modified receptors are activated by small molecules...some GPCRs further to respond to specific, pharmacologically-inert, small molecules. These new receptors...

  16. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...professors, and mentors. Ask for specific guidance on grammatical errors, sentence structures, and overall format...support.        5. Application submission  Set a calendar reminder for every deadline and shoot to submit...Pierre is currently a doctoral candidate in the Chemical Biology Program at Harvard. Roo wants to simplify...simplify access to academic resources among economically disadvantaged populations. Roo is the co-founder...resources that can help their children thrive academically. Rose is the co-founder of STEAMid.     Additional...

  17. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    Published
    Dec. 6, 2018, 1:34 p.m.
    ...plasmids can be used to carry out any biochemical and biophysical study involving protein stability or ...degradation. Despite how easy these experiments are to physically set up in the lab, RNAi is often completely ineffective... most scientists use mutant animals that are genetically altered to overexpress endogenous RNAi pathways...facilitate protein-protein interactions and mediate biochemical processes. One of the largest and best characterized...

  18. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type
    Blog Post
    Published
    July 28, 2015, 2:30 p.m.
    ...transcription factors, starting with an established method called ChIP-seq. ChIP-seq relies on antibodies to recognize...We’ve been doing that for years using this assay called ChIP-seq, but it’s really hard for lots of transcription... the second plasmid inserts a small epitope tag called FLAG at the end of the transcription factor. It...have good antibodies. The results look almost identical. Addgene: What advantages does your system have...happy to provide them, that never works on a large scale. The world is too big now. Mendenhall: So many groups...

  19. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...missing or damaged nucleotides that are common to biological sources of DSBs, and which need to be processed...generated in the course of repair by NHEJ are typically small (1-10 bp) but extremely heterogeneous. There.... NHEJ can also be engaged by variants of the canonical Cas9 approach. A pair of CRISPR guides that flank...Translocations in Human Cells Are Generated by Canonical Nonhomologous End-Joining. Molecular Cell 55:829...CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens        ...

  20. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...donating and receiving labs)” — Jim Woodgett, biomedical researcher at Lunenfeld-Tanenbaum Research Institute...reagents and protocols so easy!” — Nina Jain, Harvard Medical School. We want to give a special thanks to the...decade. We did this the old-fashioned way: through physical outreach, visiting organizations, knocking on ...standardized agreements and electronic signatures to dramatically speed up MTA approvals. In 2013 we were thrilled...for being Addgene. You guys rock." — Andrew York, Calico Life Sciences. Most of all, we’re incredibly thankful...
Showing: 381 - 400 of 847 results