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... happen before you even receive the product? How can we consider the environmental impact of those processes... waste directly into the oceans.As consumers, we can choose products that are better for the environment...sacrificing performance or quality. To do this, we can use the ACT label, which is an eco-nutrition label...sciences, some of these plastics are high grade and can be washed and reused. This might sound risky in terms... incentives (for example, by grant agencies), we can shift toward a more sustainable one. The math on ...

...smartphone apps you can use in the lab. Whether you’re tired of calculating dilutions or you can’t quite remember...chemical in your obscure growth medium, these apps can help make your lab life a bit easier. Since the post...University of Alberta and describes a technique you can use to find phage proteins that bind to your bacterium...phages are viruses that infect bacteria and that can do so with exquisite specificity (check out our interview... dress up as Mr. Incredible, an added bonus. You can find the rest of our September posts in chronological...post highlights a rare disease, Fibrodysplasia ossificans progressiva (a.k.a. FOP), that, while affecting...Chemistry & Small Molecule Fluorophores Addgene's Cancer Collection Pages Connect the Research Community...

...should be no secret that one of the best things you can do during your training is meet interesting people...a job. Ask openly to be connected to someone who can help you land that position such as a direct connection...Interviews are best conducted in person. Perhaps you can offer to buy coffee or lunch at a place convenient...and disappointments. These more personal questions can elicit the most valuable insights you will get from...each Informational Interview with the question, “Can you connect me to anyone else you think I should ...5-12 years worth!) with academic scientists who can’t help us learn about the nonacademic sphere where...

...email reminders. These programs can be synced to your online calendar and can be accessed from your computer...Organizing your lab notebooks, protocols, and to do lists can be easier than you think! Here are three tips to ... up out of the top of the notebooks, so that you can easily spot the colors and text. Tip 2: Use electronic...from anywhere, but also allow you to control who can edit the documents and track the revision history...travel, and you can’t remember where you put your flow cytometry protocol. Or maybe you can’t find the molecular...

...competition, where you can get all your burning AAV questions answered.  Interested? You can learn more here ...travel prize in our open data extravaganza!  Anyone can submit data from their CLOVER lab or Addgene AAV ...

...editing tool. However, Cas9’s endonuclease activity can be limited by the need for a specific PAM (protospacer...bind the target sites for editing. PAM sequences can be from 2-6 base pairs in length and follow the DNA...versatility of the engineered SpCas9-NG and its variants can be used to engineer specific sites that were previously...Anion-conducting channelrhodopsins (ChR) are ion channels that can be used to silence the activity of neurons. Earlier...cysteine.  With the introduction of MerMAIDs, neurons can be turned off quickly and with high precision - a... Anchor. Using specifically designed gRNAs, they can then recruit these LADL Anchors to genomic targets...

...Temperature: You can place your reaction on ice if you are planning to transform right away OR you can store the...group from a different DNA strand comes along, it can attack this covalent bond thus joining the two DNA... TOPO Cloning Reaction into Competent Cells: You can use your standard lab protocol for this; however,...and Analyze 10 White or Light Blue Colonies: You can confirm the presence of your insert by PCR, restriction...when the PCR product and vector ligate; however, it can potentially rebind and nick the newly ligated DNA...minutes (incubating the full 30 minutes will not significantly improve the transformation efficiency).  5. ...

...getting buy-in when delegating can help ensure successful results.  Can you provide the information and...you are giving this responsibility to someone who can do the job. It is good to delegate so the person ...result in more engaged, successful employees who can accomplish great things.   To read more on this ... excellent blog on delegation tips and practices can be found at the Time Doctor Blog "The Art of Delegation... How Volunteering at a Professional Organization Can Help You Develop Leadership Skils Set Concrete Goals...manager must do. You can’t delegate the giving of feedback or performance review. You can’t delegate tasks ...

...Diagnostic restriction digest Diagnostic digests can be used to confirm the rough structure of the plasmid...features within the plasmid. Restriction analysis can also be used successfully even if you don't have ... Once you have purified plasmid DNA, this method can be done right in your lab in less than a day. Diagnostic...as well as the predicted size of the insert. You can search NCBI for YGOI to find the particular reference...the dyes provide a visual reference point so you can easily assess how far the gel has run. Bonus: The...The dyes also run at predicted sizes so you can estimate how far down the gel your bands have traveled ...

...strong problem solving skills alone can take you - just because I can resolve issues effectively does not...problem solving skills” and look at how scientists can develop and highlight these valuable skills. Hopefully...found that taking a conscious or active approach can be hugely helpful not only for developing a skill...entire problem solving process on your own, but you can start by paying attention to how your mentors reach...solutions and ask them to explain their logic. Or you can try applying the scientific method to everyday problems...positions. Highlighting your problem solving skills can help convince a future employer that you are well...

... Always wear gloves, as the RNases on your hands can degrade RNA. Keep a clean work area, which may include...to remove residual DEPC. Alternatively, glassware can be baked at 180°C for at least 4 hours.   If your...layer. The top aqueous layer is extracted and RNA can then be isopropanol precipitated. This method reduced...samples cold until homogenization and cell lysis. This can be challenging depending on your laboratory situation...TRIzol® and ammonium sulfate stabilizing solutions can be made in house.   A common problem with no-kit ... extraction methods is the carryover of DNA that can potentially complicate results of a downstream application...expression. There are several things that researchers can do to combat this issue. First and foremost, be mindful...

...suitable for both short-and long-lived proteins, and it can even distinguish between protein variants when used...antibody works well. In larger cell populations, you can deliver these components together using electroporation...protein knockdown experiments! The TRIM-Away plasmids can be found here. Clift et al. Cell. 2017. PubMed PMID...which sgRNAs are used with FIRE-Cas9, experiments can be designed to target multiple loci at once. In addition...memory at nearly any locus. The FIRE-Cas9 plasmids can be found here. Braun et al. Nat Communications. ...encoding synthetic yeast transcription factors that can independently control expression of your genes of...progesterone do not affect each other, these systems can be used together. Aranda-Diaz et al additionally ...

...about being a writer that can be a very painful process. And certain stories can make you feel like ‘I’m...shared with a postdoc, and I’m like, “Oh my god, what can I do?! This is a mistake! Why did I even come [to...that’s something that good storytellers in science can do.” Susan’s number one piece of advice for getting...communication: “Find as many opportunities as you can to get your name on things.” Susan personally found...series, but also being my first interview ever! You can follow her on Twitter @sejkeown. If you enjoyed this...Susan’s NEI internship. Application information can be found here Find tips for using social media as ... staff writer at the non-profit Fred Hutchinson Cancer Research Center. Susan Keown’s science communication...

...we learned how the Cre-loxP recombination system can be used to induce site-specific recombination events...flox your genes of interest, a powerful FLEx switch can be created for your specifc experimental needs. ...and lox511 are recognized by Cre but lox511 sites can only recombine with other lox511 sites, not with ...Once the SSR of choice is introduced, recombination can proceed either by first utilizing the loxP sites ... single gene deleted in your knockout mouse, you can design a FLEx switch conditional knock-in cassette...rescue experiment with a wildtype copy of a gene, you can also design FLEx switches to conditionally test the...orientation in the presence of Cre. A similar strategy can be employed for "Cre-off" (i.e. your gene of interest...

...need. You can find all the hot plasmids from 2017 below. With over 50,000 plasmids, we can't write posts... a valuable tool for the research community. You can find the AAV vectors for infecting GABA-ergic interneurons...control of enzymatic activity. In addition, proteins can be “caged” by fusion to specific domains thereby ...mCherry variants to the repository. These variants can be used as tools to learn about the influence of ...interest. NHEJ- or HDR-induced mutations in ATP1A1 can render cells resistant to the small molecule ouabain...detailed protocol at Protocol Exchange. Plasmid maps can be found at Addgene and in Figure S12 of the publication... ladders (aka DNA molecular weight markers) that can be used to measure the size of DNA fragments. Depending...

...response to an experimental treatment can be monitored. This can reveal previously unknown functions for... specific tissues such as blood cells or neurons can be fluorescently labeled, allowing researchers to...mutagenesis screens. Microscopy-based lineage tracing can help illuminate how signaling pathways—such as those...abnormal cell types. Sequencing-based lineage tracing can help elucidate the gene networks that transform zygotes...neuroscience investigations. For example, neuronal activity can be tracked in whole brains of swimming, actively ...manipulation of brain activity: optogenetic approaches can be used to determine the consequences of activating...Lineage tracing studies highlight how these advantages can translate into insights in developmental biology,...

...parents who can answer some of the surprisingly technical questions elementary students can ask. It doesn... could contribute to the school, too. Though you can recruit parents from any background, it really helps... our math tables!  Be realistic about what you can teach  5-10 year olds in a single evening. Grades...STEM activity fun,” but rather “let’s see how we can make this fun activity relate to STEM.” Figure 1 ...teachers and other science communicators.  What can CRISPR do? I spent more time on this section of the...way to make this section more interactive. If you can think of a good analogy that involves student participation...engineering technology is moving along much faster than it can be officially regulated. I did not try to sugarcoat...

...and sgRNAs can be found here. Neuron-optimized viral vectors Alternatively, Cas9 activators can be delivered...However, using CRISPRa to target individual genes can be relatively laborious because multiple plasmids...expression. Constitutive or inducible Cre recombinases can be introduced by AAV or by mating with Cre-expressing...distinct transcriptional start site. Because CRISPRa can be targeted anywhere in the genome, it is relatively...BDNF protein. To demonstrate the specificity that can be achieved with CRISPRa, the Day lab singled out...The Day Lab’s neuron-optimized lentiviral vectors can be found here. High-throughput screening strategies...used by the Qi Lab for this particular application can be found here.  Why CRISPRa? Although cDNA overexpression...

...senior faculty members who can help you identify overly ambitious goals and can help you distinguish mechanistic...question, the methods, the tools or techniques that can help resolve the question, the anticipated outcomes...the validity of a single model. The first approach can lead to new directions, while the second approach...-like mini-organs or organoids, neither of these can substitute for a liver transplant. My lab will combine...problem. Creating a coherent unified story and model can help make your ideas easier to understand and more...outside your lab and outside your field. If they can understand and are engaged by your presentation, ... getting back to your story. Committee questions can derail your talk. You need to balance respectfully...

...Plasmids need antibiotic resistance genes so that you can specifically isolate bacteria containing your constructs... division. These non-plasmid-containing bacteria can compete with and confound the goal of your experiment...transformation (the dreaded 60 minutes shaking at 37 degrees) can be shortened since the plated cells will have time...plasmid and its associated resistance gene. They can grow proximal to a colony that does contain the plasmid... benefit it offers is marginal. Fortunately, amp can easily be replaced with carb if satellite colonies...compared to streptomycin. Spectinomycin/streptomycin can be successfully used for plant and bacterial culture...genes in their genomes. If you need a plasmid that can go between plants and bacteria, it’s a great option...