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We narrowed to 610 results for: CaS;

Showing: 381 - 400 of 610 results

  1. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ... process, following sequencing and assembly. In cases where the QC team suspects an assembly issue, they...and be made available for distribution. In these cases, the QC team leaves a note on the plasmid page to...

  2. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ... Let’s get started! Before you begin In the best case, you know ahead of time that you want to probe your... to lose by trying to strip and reprobe. In this case, we recommend using mild stripping solution (see...

  3. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ... that expresses well, then probably not. In this case, take a look at the secondary antibodies that your...to probe multiple targets in parallel. In these cases, it is critical that users choose antibodies that...

  4. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...breaches such as the Tuskegee syphilis experiment, the case of Henrietta Lacks, the Havasupai incident, and ...schools, community events, churches or mosques. On occasion, we also get interviewed for print media, radio...

  5. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...the paternal for Prader-Willi syndrome). In both cases, the other copy of the chromosome is normally imprinted... exact genes, being improperly silenced in both cases. In both diseases, genes from both copies of chromosome...

  6. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...your intellectual freedom and integrity. (Not the case, as I’ve discovered.) As someone who has now seen...academia, as many of us know, this is not always the case. There are “difficult personalities” in every work...

  7. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ... providing feedback is needed for that. In many cases reports are not aware of issues until you bring ...what to do. Unfortunately this is not always the case or you may not feel comfortable reaching out to ...

  9. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...potentially costly set-ups. However, there are many cases where the specific viral particles you need are ... viruses are too high for your budget. In those cases, you’ll need to produce your viruses in-house. ...

  10. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...can be faster and easier to work with. In either case, it can be useful to run a restriction digest of...   For CRISPR libraries, Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...

  11. Antibodies 101: Flow Compensation

    Type
    Blog Post
    Published
    May 16, 2024, 1:15 p.m.
    ...fluorophore is present in the sample (APC in the case of Figure 1). In Figure 1A, the population needs...shifted, consider your perpendicular lines. In this case, the population on the left is a horizontal line...

  12. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...hits come from these known impurities, as was the case for clean sample AAV-44362. In addition to these...containing the suspected contaminating sequence. In most cases, the source of the contamination can be identified...

  13. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...that the emission wavelength can change. In a few cases, the initial state of the protein is non-fluorescent... yield of the protein, divided by 1000. In many cases the brightness is compared to that of EGFP which...

  14. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...the sample to allow for even expansion.  In some cases, labels are added after this stage.  Step 4: Expand...alleviated by maximizing label density. In some cases, nanobodies have been found to help improve label...

  15. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...responsible for each figure. Others still are more casual with a single presenter summarizing the paper’s...could be solicited via multiple avenues including casual request via email, in person, or instant messaging...

  16. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...activation sequence (UAS) of its target gene, in this case a reporter gene (e.g. luciferase or GFP), regardless... system has been devised (8). References 1. Casadaban M.J., Martinez-Arias A., Shapira S.K., Chou J....

  17. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ... adults alike enjoyed some friendly -or in some cases, serious-  competition with our corn toss boards...word of mouth - be sure to bring up your event in casual conversation to get people excited!    Laura ...

  18. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...other tools in conjunction with the ELN, in some cases the integrations provide a direct boost to reproducibility...restrictions based on permissions, however, in some cases it would be possible to grant those wanting to understand...

  19. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...relies on dimerization for activation, only in this case ligand binding would bring the relevant ends together...RNA-based scaffolds to contain and stabilize Broccoli cassettes, thereby extending their use into mammalian cells...

  20. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...research being published on our own topic. Only on occasion do we then have time to read about research from...video abstracts in science journals: An overview and case study." Journal of Librarianship and Scholarly Communication...
Showing: 381 - 400 of 610 results