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  1. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...your intellectual freedom and integrity. (Not the case, as I’ve discovered.) As someone who has now seen...academia, as many of us know, this is not always the case. There are “difficult personalities” in every work...

  2. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...can be faster and easier to work with. In either case, it can be useful to run a restriction digest of...   For CRISPR libraries, Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...

  3. Antibodies 101: Flow Compensation

    Type
    Blog Post
    Published
    May 16, 2024, 1:15 p.m.
    ...fluorophore is present in the sample (APC in the case of Figure 1). In Figure 1A, the population needs...shifted, consider your perpendicular lines. In this case, the population on the left is a horizontal line...

  4. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...hits come from these known impurities, as was the case for clean sample AAV-44362. In addition to these...containing the suspected contaminating sequence. In most cases, the source of the contamination can be identified...

  6. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...that the emission wavelength can change. In a few cases, the initial state of the protein is non-fluorescent... yield of the protein, divided by 1000. In many cases the brightness is compared to that of EGFP which...

  7. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...responsible for each figure. Others still are more casual with a single presenter summarizing the paper’s...could be solicited via multiple avenues including casual request via email, in person, or instant messaging...

  8. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...the sample to allow for even expansion.  In some cases, labels are added after this stage.  Step 4: Expand...alleviated by maximizing label density. In some cases, nanobodies have been found to help improve label...

  9. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...activation sequence (UAS) of its target gene, in this case a reporter gene (e.g. luciferase or GFP), regardless... system has been devised (8). References 1. Casadaban M.J., Martinez-Arias A., Shapira S.K., Chou J....

  10. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ... adults alike enjoyed some friendly -or in some cases, serious-  competition with our corn toss boards...word of mouth - be sure to bring up your event in casual conversation to get people excited!    Laura ...

  11. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...relies on dimerization for activation, only in this case ligand binding would bring the relevant ends together...RNA-based scaffolds to contain and stabilize Broccoli cassettes, thereby extending their use into mammalian cells...

  12. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...research being published on our own topic. Only on occasion do we then have time to read about research from...video abstracts in science journals: An overview and case study." Journal of Librarianship and Scholarly Communication...

  13. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...copy number is high (del Solar et al., 1998).  In cases of compatible plasmids, different negative regulation...replication or partitioning machinery (Novick, 1987). In cases where one plasmid outcompetes the other, usually...

  14. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...the first time. They can then plan accordingly in case you have questions or need a little assistance at...questions later – maybe even the next day. In the latter case, you can say “I usually need time to gather my thoughts...

  15. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...hydrogen bonds holding the DNA together, it recruits helicases to continue unwinding the strands. This creates...present in the chromosomal DNA ensure that the helicases are released from the DNA once the replication...

  16. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...other tools in conjunction with the ELN, in some cases the integrations provide a direct boost to reproducibility...restrictions based on permissions, however, in some cases it would be possible to grant those wanting to understand...

  17. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...initially isolated. Regrettably this is not usually the case if you obtain a cell line from a colleague. Time... been directly isolated from patients. In these cases, a test known as Short Tandem Repeat (STR) profiling...

  18. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...density within a reasonable time-frame. For these cases, some strains carry an additional measure of control... plasmid contains a chloramphenicol resistance cassette for positive selection and a p15A origin of replication...

  19. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...approaches to record keeping. Top priorities in most cases include IP protection, publications and funding....site licenses might be available as well. In most cases this applies to universities. How are you currently...

  20. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...is that my suggestions can help you make the best case for your candidacy. A major reason that a lot of...encouraged me to publish this manual and earn some cash for my expensive habits of running shoes and single...
Showing: 381 - 400 of 600 results