We narrowed to 659 results for: Clu
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TypeBlog PostPublishedJan. 4, 2022, 2:15 p.m....everyone who visits your poster to agree with your conclusions. That conversation you’re having--that conflict...of the conflict. It can be easy to leap to the conclusion that a conflict is personal (”Person A and Person...
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Antibodies 101: Single Chain Fragment Variables (scFvs)
TypeBlog PostPublishedJune 3, 2021, 1:15 p.m....insoluble inclusion bodies. While it’s possible to re-solubilize and re-fold scFvs from inclusion bodies,... -
Tips for Getting a Faculty Position
TypeBlog PostPublishedJuly 20, 2023, 1:15 p.m....easy to follow seminar See item 2. Attendees will include grad students (that are solicited for feedback)...Howard Hughes Medical Institute. His interests include the quality control of secretory proteins in the... -
Filming Science Videos in the Age of Social Distancing
TypeBlog PostPublishedMay 20, 2021, 1:15 p.m....Build: Jennifer created a mobile camera area that included iPad and her laptop raised on an ironing board... the camera, to control some basic functions, including starting and stopping recording). Test: It was... -
10 Steps to a Perfect Science Talk
TypeBlog PostPublishedAug. 23, 2016, 2:30 p.m....if some of your work does relate, make sure to include that material, but don’t force it. You can always... Having answer slides ready after your formal conclusion slide will also help with your confidence. It... -
Using Phosphoserine to Study Protein Phosphorylation
TypeBlog PostPublishedJune 23, 2016, 2:30 p.m....appreciated. PhosphoSitePlus, one of the most inclusive databases of post-translational modifications,...pSer-incorporated proteins on a single plasmid. This includes 4 copies of a tRNA that specifically recognizes... -
Plasmids 101: Common Lab E. coli Strains
TypeBlog PostPublishedNov. 7, 2014, 2:56 p.m.... search suggests there are hundreds. This only includes general lab strains designed for subcloning or...or protein expression. If you were to include customized strains, the number is probably in the thousands... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog PostPublishedJune 24, 2020, 5:45 p.m....which uses light to regulate transcription, also includes light-regulated histone methyltransferases and...has deposited mammalian expression constructs including pcDNA-dCas9-p300 Core and pcDNA3.3-Nm-dCas9-p300... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog PostPublishedDec. 17, 2020, 1:30 p.m....Cre recombinase contained within the knock-in. Including Cre-mediated marker excision, it takes approximately...that recipients for the 2015 Breakthrough Prize included Jennifer Doudna and Emmanuelle Charpentier for... -
Important Considerations When Using AAVs
TypeBlog PostPublishedJune 13, 2017, 2:30 p.m.... utilizing chemogenetics (Designer Receptors Exclusively Activated By Designer Drugs, DREADDs) and optogenetics...examining the use of DREADDs (Designer Receptors Exclusively Activated By Designer Drugs) to determine the... -
Quick Guide to All Things Lentivirus
TypeBlog PostPublishedMarch 21, 2017, 2:30 p.m.... infect both dividing and non-dividing cells, including stem cells. These properties make lentiviral vectors..., a separate genus of the Retroviridae family including human immunodeficiency virus type-1 (HIV-1), can... -
Plasmids 101: Using Transposons in the Lab
TypeBlog PostPublishedJuly 6, 2021, 1:28 p.m....in yeast, plant, insect, and mammalian cells, including human cells. piggyBac is biased to integrate at...all been used to generate transgenic animals, including zebrafish, mice, rats, and rabbits (Sandoval-Villegas... -
A Guide to Getting Started in Undergrad Research
TypeBlog PostPublishedJune 8, 2021, 1:15 p.m.... still valuable for anyone in the lab though, including undergrads. I found a lot of career resources ...in microbes until the end of my second year! Conclusion and other pieces of advice Give yourself permission... -
9 tips for a successful postdoctoral experience
TypeBlog PostPublishedOct. 9, 2018, 12:21 p.m....stop and plan it out. Some questions to answer include: What is the goal of the experiment? What are...Howard Hughes Medical Institute. His interests include the quality control of secretory proteins in the... -
Which Fluorescence Microscopy Technique is Best for Me?
TypeBlog PostPublishedOct. 10, 2017, 1:57 p.m.... detector and all light from above or below is excluded. Parallelized Confocal Microscopy (Spinning Disk... type of sample as they are most efficient at excluding out of focus light. However, they also continually... -
How to Deposit Your Plasmids with Addgene
TypeBlog PostPublishedFeb. 27, 2024, 2:15 p.m....information related to your plasmid. It is optional to include the total size of the insert and the GenBank ID...submission, please email us at [email protected]. Include your deposit number and which data needs to be ... -
Delivery Methods for Generating iPSCs
TypeBlog PostPublishedApril 17, 2018, 1:37 p.m.... Paramyxoviridae family of viruses, which also includes measles and mumps. Sendai transduces a wide range...the field of iPSCs Find gene-specific plasmids (including reporters and RNAi) for: NANOG OCT4 SOX2 MYC... -
Plasmids 101 eBook 4th Edition
TypeBlog PostPublishedSept. 5, 2023, 1:15 p.m.... been fully updated with twelve new sections, including our popular "Dimers and Multimers" post. It's ... -
Addgene Launches Partnership with IPI
TypeBlog PostPublishedJune 13, 2023, 1:15 p.m....educational resources on our website and blog, including our antibody guide, antibody protocols, and Antibodies... -
Thanks to Addgene Depositors, We’re Now Sharing over 100,000 Reagents!
TypeBlog PostPublishedApril 8, 2021, 7:25 p.m....globe, we’re now sharing over 100,000 reagents including plasmids, ready-to-use viral preps, pooled libraries...