... table below lists plasmids that can be used to create your choice of fluorescent fusion protein with ...quenching and the fluorescence of the donor is increased. This method is straightforward and quantitative...days and good “gigs” ahead...No offense to BRET, CRET and other RET techniques which have never outclassed... BRET= Bioluminescence Resonance Energy Transfer CRET= Chemiluminescence Resonance Energy Transfer    ...

...21–26). Increased scientific funding (17). Federal funding for science has been decreasing for the last... government and, consequently, politics. More concretely, scientists gather information about reality ...institutions, while many also seek to honor and credit women, immigrants, and minorities who have had ...

...complex can bind to your biotinylated antibody. This creates a large meshwork of biotin-avidin complexes bound...label a protein of interest with biotin by simply creating a fusion in which AviTag is added to either the...or polypeptides to a solid substrate (Holden and Cremer, 2003). References 1. Chapman-Smith, A., & Cronan...588–600. PubMed PMID: 2388593 7. Holden, M. A., & Cremer, P. S. “Light activated patterning of dye-labeled...

...most unique model organisms, recognized for its incredible regeneration abilities. The axolotl has the ability..., 2019). To study regeneration scientists have created molecular biology tools to generate transgenic ...Xenopus genes and a tool-kit from the Hamdoun Lab to create fluorescent fusions in a multitude of organisms...Xing, X.-H. (2018). Pooled CRISPR interference screening enables genome-scale functional genomics study...

... an organization gets, and creating new avenues of communication can create as many problems as it can...guidance X and Y might be able to work together to create solution C. And if a conflict resolver does their...have learned that by working together, they can create unique solutions that benefit themselves and their...

...phosphoserine (Sep)-tRNA synthetase enabled researchers to create an orthogonal translation system (OTS) for the ...residues in the above study with phosphoserine (to create a pSer/phosphomimetic dual mutant) elevated kinase... as an alternative to phosphomimetic mutation. Creating phosphoserine-incorporated proteins The generation... This is a particularly important control when creating a phosphoserine-incorporation mutant for a residue...

...be cloned into E. coli and subsequent proteins screened for cell-binding abilities (6). This can be accomplished... repair sheared ends with T4 DNA polymerase (to create blunt ends) and ligate into a bluntly digested ...construction for functional genomic and metagenomic screening." Applied and environmental microbiology 74.5 ...Protein-Protein Interactions Use CRISPR For Genome-Wide Screens Resources on Addgene.org Visit Our Microbiology...

...Experimental output is measured and quantified. Created with BioRender.com.   Optogenetic tools Types...passing ions against the concentration gradient. Created with BioRender.com.   Opto-G-protein coupled...the binding pocket and activates the receptor. Created with BioRender.com.   Controlling secondary...LOV domain allowing the pathway to be activated. Created with BioRender.com.   Important considerations...

...differences. If you are performing CRISPR library screens, the same principle holds true - the higher the...lower the likelihood of an off-target artifact. Increasing the N in your experiment is the only way to effectively...effectively control for off-targets and significantly decrease the probability of artifacts. Off-target controls...Rabinowitz R, Offen D. Single-Base Resolution: Increasing the Specificity of the CRISPR-Cas System in Gene...

...individuals wherever the cell cultures are kept will increase the risk of contamination. Mycoplasmas can spread...even though the viability of cryopreserved cells decreases with every subculture. Equipment Fumigate the... not be stored in a single incubator since it increases the risk of cross-contamination. CO2 incubators...Elimination is a very time-consuming process and increases the risk of secondary contamination to other healthy...

...The premise of the novel involves Dr. Moreau who creates human-animal hybrids. This book is published 30...character revolts against class divide that is created by genetics.    Zinc finger nucleases Zinc finger... it can read many base pairs simultaneously to increase specificity. This modular approach made ZFNs highly...space travels. The movie sees the USS Enterprise crew fight against a genetically engineered superhuman...

...IWGAV five proposed antibody validation pillars. Created with BioRender.com.     Orthogonal Strategies...knock-down expression of your target or CRISPR/Cas9 to create mutations that essentially eliminate expression...opposite of the Genetic strategy and looks for increased antibody signal in samples that have had the target... target protein expression experimentally increased. The simplest version would be to use cells that do...

...public. Find out more at www.projbridge.com. Walnut Creek, CA, USA 37.9101°N 122.0652°W – Steph Hays When...microbial interactions, and science education. Photo cred Alina Chan. Seattle, Washington, USA 47.6062°N 122.3321...others come out who feel the same.  I was also incredibly impressed with how well organized the march was...was, as well as the friendly atmosphere that was created by the many thousands of science professionals ...

...Parallelized Confocal Microscopy (Spinning Disk) increases the speed at which a confocal image can be acquired...utilizes a configuration of two or more objectives to create a thin sheet of excitation light that propagates...molecules on and off. This can provide a 2-10 fold increase in resolution, down to 10s of nanometers. Thick...samples due to its fast temporal resolution and decreased phototoxicity. Lightsheet designs that allow for...

...more photons are absorbed and then emitted, which creates a relatively stronger — or brighter — signal.   ...brightness levels on your smartphone or computer screen), and you should be able to properly label many...included—the live/dead stain. This stain is in fact not created through the actual APC-Cy7 fluorophore but displays...absorbed and emitted by matter, and how this process creates visible colors. Next, you learned about how fluorophores...

...year’s impressive entries, but our community of creative scientists delivered some spectacular photos once...supply tree and set a challenge for her lab mates to create the best-recycled lab supply ornament ever! & they...winners. We hope everyone had a blast decorating and creating memories with their labmates! Are you inspired...

...the world to help increase global access to materials in our repository. This incredible gift will allow...a los materiales de nuestro repositorio. Este increíble regalo permitirá que más personas accedan a recursos...

...complement our written protocol materials, we have also created video protocols.  These feature step-by-step instructions...below: How-to videos Our How-To videos feature screen-capture footage to help you navigate Addgene’s ...the Addgene Blog 5 Science Rap Videos and Their Creator: Tom McFadden SciComm with the Experts at Science...

...understand the features of the plasmids, how they were created, and how to use them in experiments. Best practices...magical Addgene badge appear? First you’ll need to create an account on PubMed. Once you have an account,...populate your “PubMed filter list” to the left of your screen. That’s it! Next time you log-in to your PubMed...

...science has been hot this year and how do you creatively turn it into an outfit?), and of course there...bragging rights for the next 12 months. Your team’s creativity, cleverness, and crafting skills have surpassed...in 2012. The Office Team was back up to their creative hijinks last year. They showed us their dedication...