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We narrowed to 798 results for: Cre

Showing: 381 - 400 of 798 results

  1. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ... bound by QCascade (Fig. 1B). The process also creates a 5-bp target-site duplication (TSD), a hallmark...Transform plasmid(s) into cells and plate overnight Screen colonies phenotypically or by PCR/sequencing ...doi.org/10.1073/pnas.1709035114 Rubin BE, Diamond S, Cress BF, Crits-Christoph A,  He C , Xu M, Zhou Z, Smock...science.abb2920 Vo PLH, Ronda C, Klompe SE, Chen EE, Acree C, Wang HH, Sternberg SH (2020) CRISPR RNA-guided...

  2. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ...teamwork, communication, writing, management, and creativity; I have developed these skills through school...not just “do the job”. When they ask about your creativity, your potential manager wants to hear a specific... job market, it is the transferable skills that create a full package and add an extra shine to your work...resolution Organization Leadership Problem Solving Creativity Data analysis Listening Negotiation Career Planning...

  3. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...purpose of MXS chaining MXS-chaining was designed to create plasmids for fluorescence imaging or flow cytometry...cells. Four individual expression constructs were created, each containing a unique fluorescent protein with...) and the four constructs were then combined to create a single polycistronic 15 kb insert. The final ...construction of plasmids with many components. The creators of the MXS-chaining method have used the technique...

  4. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ...written portion of the grant application. They have increased their demand for stronger budgets with clear budget..., the amount of grant funds available and the increasing regulatory process for grants has fostered the... your own Professional Development Strategy to increase your competencies and skills in the grants profession...Management and Evaluation and/or earning the GPC credential from the Grant Professional Certification Institute...

  5. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...other epitope tags – which when doubled or tripled increase the tag size quickly – modifying the length a ...tag is not enough. What if you need one tag to increase solubility and one tag for purification? Or you...don’t want more tag than protein), 2-3 tags is increasingly common. Tandem affinity purification (TAP) once...protease itself, with various mutations used to increase its stability activity, can be readily purified...

  6. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ... sequences Restriction sites Sequences prone to create secondary structures in RNA transcripts Effects...factors on their own. Luckily, many researchers have created codon optimization algorithms and DNA synthesis...abundance tRNAs in your expression host and thereby increase their abundance. For example, the commercial Rosetta...system for many different genes without having to create new constructs. However, due to problems such as...

  7. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...inefficient, and often not reproducible. Prior to creating transgenic cultures or plants, our transient expression... expression method enables us to quickly screen and investigate transcription factor candidates and the...with the MoClo system you can easily and quickly create customized vectors. For more information on MoClo...transient leaf transformations are a great help in screening candidate genes and speeding up research. Certainly...

  8. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...s clinical and commercial future, and yet are incredibly difficult to find solutions to. There are many...on them in a timely fashion while allowing for creative solutions to emerge. They enjoy the fact that ...home and at the office. Consulting firms are increasingly aware of the toll that long working hours and...career paths available to MDs and PhDs, and an increasing recognition of the value graduates bring to these...

  9. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ... table below lists plasmids that can be used to create your choice of fluorescent fusion protein with ...quenching and the fluorescence of the donor is increased. This method is straightforward and quantitative...days and good “gigs” ahead...No offense to BRET, CRET and other RET techniques which have never outclassed... BRET= Bioluminescence Resonance Energy Transfer CRET= Chemiluminescence Resonance Energy Transfer    ...

  10. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...21–26). Increased scientific funding (17). Federal funding for science has been decreasing for the last... government and, consequently, politics. More concretely, scientists gather information about reality ...institutions, while many also seek to honor and credit women, immigrants, and minorities who have had ...

  11. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ... an organization gets, and creating new avenues of communication can create as many problems as it can...guidance X and Y might be able to work together to create solution C. And if a conflict resolver does their...have learned that by working together, they can create unique solutions that benefit themselves and their...

  12. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...most unique model organisms, recognized for its incredible regeneration abilities. The axolotl has the ability..., 2019). To study regeneration scientists have created molecular biology tools to generate transgenic ...Xenopus genes and a tool-kit from the Hamdoun Lab to create fluorescent fusions in a multitude of organisms...Xing, X.-H. (2018). Pooled CRISPR interference screening enables genome-scale functional genomics study...

  13. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...complex can bind to your biotinylated antibody. This creates a large meshwork of biotin-avidin complexes bound...label a protein of interest with biotin by simply creating a fusion in which AviTag is added to either the...or polypeptides to a solid substrate (Holden and Cremer, 2003). References 1. Chapman-Smith, A., & Cronan...588–600. PubMed PMID: 2388593 7. Holden, M. A., & Cremer, P. S. “Light activated patterning of dye-labeled...

  14. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...phosphoserine (Sep)-tRNA synthetase enabled researchers to create an orthogonal translation system (OTS) for the ...residues in the above study with phosphoserine (to create a pSer/phosphomimetic dual mutant) elevated kinase... as an alternative to phosphomimetic mutation. Creating phosphoserine-incorporated proteins The generation... This is a particularly important control when creating a phosphoserine-incorporation mutant for a residue...

  15. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...differences. If you are performing CRISPR library screens, the same principle holds true - the higher the...lower the likelihood of an off-target artifact. Increasing the N in your experiment is the only way to effectively...effectively control for off-targets and significantly decrease the probability of artifacts. Off-target controls...Rabinowitz R, Offen D. Single-Base Resolution: Increasing the Specificity of the CRISPR-Cas System in Gene...

  16. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...Experimental output is measured and quantified. Created with BioRender.com.   Optogenetic tools Types...passing ions against the concentration gradient. Created with BioRender.com.   Opto-G-protein coupled...the binding pocket and activates the receptor. Created with BioRender.com.   Controlling secondary...LOV domain allowing the pathway to be activated. Created with BioRender.com.   Important considerations...

  17. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...be cloned into E. coli and subsequent proteins screened for cell-binding abilities (6). This can be accomplished... repair sheared ends with T4 DNA polymerase (to create blunt ends) and ligate into a bluntly digested ...construction for functional genomic and metagenomic screening." Applied and environmental microbiology 74.5 ...Protein-Protein Interactions Use CRISPR For Genome-Wide Screens Resources on Addgene.org Visit Our Microbiology...

  18. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...The premise of the novel involves Dr. Moreau who creates human-animal hybrids. This book is published 30...character revolts against class divide that is created by genetics.    Zinc finger nucleases Zinc finger... it can read many base pairs simultaneously to increase specificity. This modular approach made ZFNs highly...space travels. The movie sees the USS Enterprise crew fight against a genetically engineered superhuman...

  19. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    Published
    Oct. 8, 2020, 1:15 p.m.
    ...individuals wherever the cell cultures are kept will increase the risk of contamination. Mycoplasmas can spread...even though the viability of cryopreserved cells decreases with every subculture. Equipment Fumigate the... not be stored in a single incubator since it increases the risk of cross-contamination. CO2 incubators...Elimination is a very time-consuming process and increases the risk of secondary contamination to other healthy...

  20. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...IWGAV five proposed antibody validation pillars. Created with BioRender.com.     Orthogonal Strategies...knock-down expression of your target or CRISPR/Cas9 to create mutations that essentially eliminate expression...opposite of the Genetic strategy and looks for increased antibody signal in samples that have had the target... target protein expression experimentally increased. The simplest version would be to use cells that do...
Showing: 381 - 400 of 798 results