We narrowed to 694 results for: EED
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TypeBlog PostPublishedFeb. 16, 2021, 2:15 p.m....master’s degree. I wanted to be a scientist and you need a PhD to be a scientist, or so I thought. So I entered...s-level job postings on a job search website like Indeed. Do the job descriptions sound interesting? Is ...
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Chemogenetics vs. Optogenetics: Which Method Should I Choose?
TypeBlog PostPublishedJune 11, 2020, 1:34 p.m....Invasiveness Both optogenetic and chemogenetic tools need to be delivered to cells, commonly through viral...Acknowledgements We thank the Bryan Roth lab for feedback on this article and sharing their expertise with... -
Fostering communication in remote collaborations
TypeBlog PostPublishedApril 21, 2020, 1:14 p.m....collaboration and immediately discuss the feedback with everyone involved can speed up the future directions of the... -
Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process
TypeBlog PostPublishedFeb. 1, 2018, 3:07 p.m....amount of guanine and cytosine nucleotides, we might need to send it for Sanger sequencing to verify the sequence...repetitive region is present in the plasmid, we might need to perform a restriction digest to confirm the size... -
Choosing a Good Mentor for Scientists
TypeBlog PostPublishedJan. 16, 2014, 2:56 p.m....advisors they choose will be ones that help them succeed after the training period. While there are many...is important in both cases. As a PhD student you need to learn how to be a scientist. Your PI should be... -
Antibodies 101: Chimeric Antibodies
TypeBlog PostPublishedApril 25, 2023, 1:15 p.m....isotope conversion, gives users the flexibility needed for multiplexing. In the case above, you can’t ...this will not affect single-plexed experiments, it needs to be addressed when multiplexing. It is imperative... -
10 Great Guest Posts We're Thankful For
TypeBlog PostPublishedNov. 18, 2016, 3 p.m....many microns in response to pH changes without the need for ATP or other chemical fuel sources.” In her ... how you can customize it for your experimental needs. Note: Casilio is no longer available through Addgene... -
Viral Vectors 101: Parts of the AAV Transfer Plasmid
TypeBlog PostPublishedAug. 11, 2020, 1:15 p.m...., making AAV starts with some plasmids. You just need three plasmids to start making AAV: the packaging...adenoviral helper plasmid which contains the proteins needed for the virus to replicate, and the transfer plasmid... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog PostPublishedNov. 12, 2019, 2 p.m....sequencing approaches, the lab was able to eliminate the need for axenic (pure) laboratory cultures to study these...Lab, volunteered to do the sampling and I happily agreed to accompany him to YNP the following year. Geoff... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog PostPublishedJune 9, 2016, 2:30 p.m....should be stable in the cell. gDNA guides will also need to be transfected into the cells of interest. In...Higher sensitivity to off-targets More research is needed to compare CRISPR and NgAgo, but it seems that ... -
Plasmids 101: A Brief History of Plasmids and an Improved eBook!
TypeBlog PostPublishedOct. 29, 2015, 2:30 p.m....DNA Cloning: A Personal View after 40 Years.”Proceedings of the National Academy of Sciences of the United...Biologically Functional Bacterial Plasmids In Vitro.” Proceedings of the National Academy of Sciences of the United... -
Advice for Moving Into Sales After Your Science Postdoc
TypeBlog PostPublishedAug. 21, 2014, 3:15 p.m....colleagues and what I was researching, but I decided I needed a new challenge. Looking for sales and marketing...opportunities to expand their skill sets?", “Have they succeeded in what they have done?” and “Would their academic... -
Hot Plasmids - January 2023
TypeBlog PostPublishedJan. 31, 2023, 2:15 p.m....another option for targeting GFP. But why might you need a second GFP antibody? Similarities: Generated...Stroik Microscopy techniques have been moving at the speed of light over the last decade, but the fluorescent... -
Early Career Researcher Toolbox: Free Tools for Science Writing
TypeBlog PostPublishedApril 28, 2021, 1:15 p.m....beautiful graphs! I like to look at these graphs when I need that extra push to stick with my writing routine.... The phrases in Phrasebank are generic, so they need to be edited to suit your particular field of research... -
Quantifying DNA? Here are Five DNA Quantification Methods to Consider
TypeBlog PostPublishedJuly 21, 2020, 1:15 p.m....absorbance based methods can’t tell you and you don’t need a spectrophotometer or a fluorometer to quantify... automated. Unlike gel electrophoresis, you only need 1-2 ul of sample and the run time is just a few ... -
"Build Your Plasmid": The Game - Play to Teach
TypeBlog PostPublishedJuly 17, 2018, 12:23 p.m....YOUR PLASMID BUILD YOUR PLASMID is a game where you need to apply basic molecular biology, cell biology, ...and one “Transfer Plasmid” game board. Once they agreed on each element to use, they had to place the selected... -
Visualizing Genomic Loci with CRISPR-Sirius
TypeBlog PostPublishedApril 18, 2019, 12:53 p.m....misfolding and recombination during virus production needed to deliver CRISPR-Sirius into cells. They named...labeling of chromosomal loci in human cells.” Proceedings of the National Academy of Sciences of the United... -
Summer SciComm Series: A PhD in Science Communication
TypeBlog PostPublishedAug. 23, 2022, 1:15 p.m....can tailor our communications to better meet the needs of the people we are talking to. Emma Harcourt ...polarized beliefs on controversial science topics. Proceedings of the National Academy of Sciences of the United... -
Advice for Scientists Starting a Lab
TypeBlog PostPublishedApril 29, 2014, 1:43 p.m.... was surprised by the variety of skill sets she needed to acquire as a professor. “Being a PI is more ...“how much better it is once it gets fully up to speed.” He also comments that it is also surprising “ ... -
Finding and Joining Your Dream Lab
TypeBlog PostPublishedApril 8, 2014, 2:50 p.m....a lab that fits both work style and development needs. These two factors will provide a solid foundation...prefer a more competitive style to spur you to succeed or you might enjoy working closely with others ...