We narrowed to 700 results for: EED

...Invasiveness Both optogenetic and chemogenetic tools need to be delivered to cells, commonly through viral...Acknowledgements We thank the Bryan Roth lab for feedback on this article and sharing their expertise with...

...collaboration and immediately discuss the feedback with everyone involved can speed up the future directions of the...

... easier for you to find and use the plasmids you need. Here’s what you’ll find in this article: CRISPR-based...catalytic activity.  As SLs are also known to trigger seed germination of parasitic plants, this approach was...

...master’s degree. I wanted to be a scientist and you need a PhD to be a scientist, or so I thought. So I entered...s-level job postings on a job search website like Indeed. Do the job descriptions sound interesting? Is ...

... Addgene has no investors or stock. All of our proceeds go into running the repository and serving scientists... about Our Collaboration with Fellow Nonprofit Seeding Labs Addgene Supports Rare Disease Research Listen...

... biosensors When choosing a biosensor, you first need to decide the best way to track or measure the process...experiment. For example, does the fluorescent tag need to be pH stable or withstand a particular solute...

...advisors they choose will be ones that help them succeed after the training period. While there are many...is important in both cases. As a PhD student you need to learn how to be a scientist. Your PI should be...

...isotope conversion, gives users the flexibility needed for multiplexing. In the case above, you can’t ...this will not affect single-plexed experiments, it needs to be addressed when multiplexing. It is imperative...

..., making AAV starts with some plasmids. You just need three plasmids to start making AAV:  the packaging...adenoviral helper plasmid which contains the proteins needed for the virus to replicate,  and the transfer plasmid...

...sequencing approaches, the lab was able to eliminate the need for axenic (pure) laboratory cultures to study these...Lab, volunteered to do the sampling and I happily agreed to accompany him to YNP the following year. Geoff...

...many microns in response to pH changes without the need for ATP or other chemical fuel sources.” In her ... how you can customize it for your experimental needs. Note: Casilio is no longer available through Addgene...

...should be stable in the cell. gDNA guides will also need to be transfected into the cells of interest. In...Higher sensitivity to off-targets More research is needed to compare CRISPR and NgAgo, but it seems that ...

...DNA Cloning: A Personal View after 40 Years.”Proceedings of the National Academy of Sciences of the United...Biologically Functional Bacterial Plasmids In Vitro.” Proceedings of the National Academy of Sciences of the United...

...colleagues and what I was researching, but I decided I needed a new challenge. Looking for sales and marketing...opportunities to expand their skill sets?", “Have they succeeded in what they have done?” and “Would their academic...

...another option for targeting GFP. But why might you need a second GFP antibody?  Similarities:  Generated...Stroik Microscopy techniques have been moving at the speed of light over the last decade, but the fluorescent...

...absorbance based methods can’t tell you and you don’t need a spectrophotometer or a fluorometer to quantify... automated. Unlike gel electrophoresis, you only need 1-2 ul of sample and the run time is just a few ...

...beautiful graphs! I like to look at these graphs when I need that extra push to stick with my writing routine.... The phrases in Phrasebank are generic, so they need to be edited to suit your particular field of research...

...misfolding and recombination during virus production needed to deliver CRISPR-Sirius into cells. They named...labeling of chromosomal loci in human cells.” Proceedings of the National Academy of Sciences of the United...

...YOUR PLASMID BUILD YOUR PLASMID is a game where you need to apply basic molecular biology, cell biology, ...and one “Transfer Plasmid” game board. Once they agreed on each element to use, they had to place the selected...

...can tailor our communications to better meet the needs of the people we are talking to. Emma Harcourt ...polarized beliefs on controversial science topics. Proceedings of the National Academy of Sciences of the United...