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  1. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    Published
    Jan. 16, 2014, 2:56 p.m.
    ...advisors they choose will be ones that help them succeed after the training period. While there are many...is important in both cases. As a PhD student you need to learn how to be a scientist. Your PI should be...

  2. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ..., making AAV starts with some plasmids. You just need three plasmids to start making AAV:  the packaging...adenoviral helper plasmid which contains the proteins needed for the virus to replicate,  and the transfer plasmid...

  3. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ...isotope conversion, gives users the flexibility needed for multiplexing. In the case above, you can’t ...this will not affect single-plexed experiments, it needs to be addressed when multiplexing. It is imperative...

  5. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...many microns in response to pH changes without the need for ATP or other chemical fuel sources.” In her ... how you can customize it for your experimental needs. Note: Casilio is no longer available through Addgene...

  6. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ...should be stable in the cell. gDNA guides will also need to be transfected into the cells of interest. In...Higher sensitivity to off-targets More research is needed to compare CRISPR and NgAgo, but it seems that ...

  7. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ...DNA Cloning: A Personal View after 40 Years.”Proceedings of the National Academy of Sciences of the United...Biologically Functional Bacterial Plasmids In Vitro.” Proceedings of the National Academy of Sciences of the United...

  8. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ...colleagues and what I was researching, but I decided I needed a new challenge. Looking for sales and marketing...opportunities to expand their skill sets?", “Have they succeeded in what they have done?” and “Would their academic...

  10. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...misfolding and recombination during virus production needed to deliver CRISPR-Sirius into cells. They named...labeling of chromosomal loci in human cells.” Proceedings of the National Academy of Sciences of the United...

  11. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    Published
    July 17, 2018, 12:23 p.m.
    ...YOUR PLASMID BUILD YOUR PLASMID is a game where you need to apply basic molecular biology, cell biology, ...and one “Transfer Plasmid” game board. Once they agreed on each element to use, they had to place the selected...

  12. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    Published
    Aug. 23, 2022, 1:15 p.m.
    ...can tailor our communications to better meet the needs of the people we are talking to. Emma Harcourt ...polarized beliefs on controversial science topics. Proceedings of the National Academy of Sciences of the United...

  13. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    ...beautiful graphs! I like to look at these graphs when I need that extra push to stick with my writing routine.... The phrases in Phrasebank are generic, so they need to be edited to suit your particular field of research...

  14. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...another option for targeting GFP. But why might you need a second GFP antibody?  Similarities:  Generated...Stroik Microscopy techniques have been moving at the speed of light over the last decade, but the fluorescent...

  15. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ... was surprised by the variety of skill sets she needed to acquire as a professor. “Being a PI is more ...“how much better it is once it gets fully up to speed.” He also comments that it is also surprising “ ...

  16. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ...a lab that fits both work style and development needs. These two factors will provide a solid foundation...prefer a more competitive style to spur you to succeed or you might enjoy working closely with others ...

  17. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...most requested plasmids into lentiviral preps to speed up COVID-19 research even further. The following...different patterns of expression, researchers often need to determine these transduction patterns empirically...

  18. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...that there was a prior version of this plasmid. Indeed the first version is the 15th most popular plasmid...packaging, reverse transcription, and integration needed for the production of lentiviral particles. It’...

  19. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...transcription >1,000-fold when activated. Lag time The time needed to induce a given promoter varies. Promoters that...inducer will have higher lag time due to the time needed for transcription/translation. Many thanks to ...

  20. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ...similar to the widely used FRET, but it eliminates the need for an excitation light source. Instead, a luciferase...Saito, Kenta, et al. “Luminescent proteins for high-speed single-cell and whole-body imaging.” Nat Commun....
Showing: 381 - 400 of 686 results