We narrowed to 687 results for: Eng

...spring requiring an overnight hike in. Navigating challenges: collecting and processing environmental samples...,241 amino acids long, has optimal sgRNA spacer length of 22-25 nts, and is thermostable between 31-100...

...using sgRNAiBAR. Image from Zhu et al., 2019.  Wensheng Wei's lab at Peking University has developed a...recombination sites in the construct to different lengths, the density of cells that are labeled can be altered...

...metagenome datasets from hypersaline samples, were engineered and adapted for RNAi experiments for mammalian...mammalian cell lines. The Turner Lab Human messenger-RBP sgRNA Library targets human mRNA binding proteins with...

...Because mNeonGreen wavelengths lie between the green and yellow fluorescent protein wavelengths, it can be imaged...

...23817127. PubMed Central PMCID: PMC3713494.  Ke, Meng-Tsen, et al. "Super-resolution mapping of neuronal...reports14.11 (2016): 2718-2732. PubMed PMID: 26972009. Ke, Meng-Tsen, and Takeshi Imai. "Optical clearing and index...

...Regardless of excitation wavelength, Keima emits at 620 nm. The ratio of signal strength excited at 586 nm to...

...controls available when needed. SnapGene has been engineered to be easy and enjoyable to use. Development ...annotation process. Many of the best ideas come from engaging with users of the software. We welcome suggestions...

...screening platform, Oliver Griesbeck’s lab set out to engineer a new FP. Their aim - to red-shift the boundaries...expansion of the genome editing toolkit. However, a challenge in optimizing base editing technologies across...

...are single domain antibody fragments typically engineered from heavy-chain only antibodies found in camelids...catalytically inactive dCas9 by fusing it to an engineered peroxidase, (APEX) that labels proximal proteins...

...other media outlets (AAAS Mass Media Science & Engineering Fellows Program). Science journalism programs...the Advancement of Science. She is enjoys broad engagement through science communication with a variety ...

...solution. 5. Cpf1: A New Tool for CRISPR Genome Engineering While the vast majority of CRISPR plasmids in...validation process for CRISPR mediated genome engineering. 10. Plasmids 101: Control Plasmids Need help...

...different wavelengths of light to different degrees and many molecules have a specific wavelength that they...

...distance.  From front to back: David Gordon, Jeff Guo, Fengbo Zhou, Paddy O’Leary (UCSF). Our approach is ...to Margaret Soucheray, Jeff Guo, Paddy O’Leary, Fengbo Zhou, Helene Foussard, and Dave Gordon) have been...

...fluorescence imaging of living organisms can be challenging due to issues with tissue autofluorescence, cell... by directed evolution. They then optimized the length of both linkers to generate a reporter that would...

...from the MIT Sloan School of Management. Professor Feng Zhang’s original 2013 gene editing paper on CRISPR...References 1. Cong, Le, et al. "Multiplex genome engineering using CRISPR/Cas systems." Science 339.6121 (...

...the Wyss Institute for Biologically Inspired Engineering and member of the Addgene Advisory board, on ...the Wyss Institute for Biologically Inspired Engineering and the Zhang and Doench/Root labs from the Broad...

...lines from becoming the new HeLa cells?), genome engineering and stem cells, and the difficulty of sharing...into a pig. In stark contrast to recent genome engineering technologies, stem cell techniques are neither...

...starting cell culture work. 3. NEB Tools by New England BioLabs In my lab we do a lot of genetic construction...experiments. Not all of our lab enzymes are made by New England BioLabs, but the NEB Tools app comes in handy when...

... Complementary to the released strand, 127bp in length, With 36bp on the PAM-distal side of the break ...al. "Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery....

...comments section below! Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.       Resources...