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TypeBlog PostPublishedApril 25, 2019, 3:01 p.m....Because mNeonGreen wavelengths lie between the green and yellow fluorescent protein wavelengths, it can be imaged...
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Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog PostPublishedJune 2, 2020, 1:15 p.m....using sgRNAiBAR. Image from Zhu et al., 2019. Wensheng Wei's lab at Peking University has developed a...recombination sites in the construct to different lengths, the density of cells that are labeled can be altered... -
Tetbow: Bright Multicolor Labeling for Neuronal Tracing
TypeBlog PostPublishedJan. 24, 2019, 2:24 p.m....23817127. PubMed Central PMCID: PMC3713494. Ke, Meng-Tsen, et al. "Super-resolution mapping of neuronal...reports14.11 (2016): 2718-2732. PubMed PMID: 26972009. Ke, Meng-Tsen, and Takeshi Imai. "Optical clearing and index... -
Fluorescent Biosensors for Measuring Autophagic Flux
TypeBlog PostPublishedJan. 22, 2019, 2:41 p.m....Regardless of excitation wavelength, Keima emits at 620 nm. The ratio of signal strength excited at 586 nm to... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog PostPublishedDec. 21, 2017, 2:06 p.m....controls available when needed. SnapGene has been engineered to be easy and enjoyable to use. Development ...annotation process. Many of the best ideas come from engaging with users of the software. We welcome suggestions... -
Addgene's Top 10 Blog Posts of 2015
TypeBlog PostPublishedDec. 7, 2015, 6 p.m....solution. 5. Cpf1: A New Tool for CRISPR Genome Engineering While the vast majority of CRISPR plasmids in...validation process for CRISPR mediated genome engineering. 10. Plasmids 101: Control Plasmids Need help... -
Addgene Depositors Get More Citations
TypeBlog PostPublishedApril 20, 2017, 2:30 p.m....from the MIT Sloan School of Management. Professor Feng Zhang’s original 2013 gene editing paper on CRISPR...References 1. Cong, Le, et al. "Multiplex genome engineering using CRISPR/Cas systems." Science 339.6121 (... -
FlipGFP, a novel fluorescence protease reporter to study apoptosis
TypeBlog PostPublishedMay 21, 2019, 12:10 p.m....fluorescence imaging of living organisms can be challenging due to issues with tissue autofluorescence, cell... by directed evolution. They then optimized the length of both linkers to generate a reporter that would... -
Quantifying DNA? Here are Five DNA Quantification Methods to Consider
TypeBlog PostPublishedJuly 21, 2020, 1:15 p.m....different wavelengths of light to different degrees and many molecules have a specific wavelength that they... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog PostPublishedApril 9, 2020, 1:15 p.m....distance. From front to back: David Gordon, Jeff Guo, Fengbo Zhou, Paddy O’Leary (UCSF). Our approach is ...to Margaret Soucheray, Jeff Guo, Paddy O’Leary, Fengbo Zhou, Helene Foussard, and Dave Gordon) have been... -
With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog PostPublishedDec. 6, 2016, 3:30 p.m....the Wyss Institute for Biologically Inspired Engineering and member of the Addgene Advisory board, on ...the Wyss Institute for Biologically Inspired Engineering and the Zhang and Doench/Root labs from the Broad... -
Four Factors that Differentiate the Stem Cell Field
TypeBlog PostPublishedSept. 28, 2016, 2:35 p.m....lines from becoming the new HeLa cells?), genome engineering and stem cells, and the difficulty of sharing...into a pig. In stark contrast to recent genome engineering technologies, stem cell techniques are neither... -
5 Great Apps for Lab Life
TypeBlog PostPublishedSept. 22, 2016, 2:30 p.m....starting cell culture work. 3. NEB Tools by New England BioLabs In my lab we do a lot of genetic construction...experiments. Not all of our lab enzymes are made by New England BioLabs, but the NEB Tools app comes in handy when... -
Optimizing Donor DNA for Enhanced CRISPR Genome Editing
TypeBlog PostPublishedMarch 24, 2016, 2:30 p.m.... Complementary to the released strand, 127bp in length, With 36bp on the PAM-distal side of the break ...al. "Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery.... -
Addgene Welcomes John Doench to the Advisory Board
TypeBlog PostPublishedAug. 13, 2015, 2:30 p.m....comments section below! Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast. Resources... -
Finding and Joining Your Dream Lab
TypeBlog PostPublishedApril 8, 2014, 2:50 p.m....scientists and technical people) is linked to engagement and access to growth opportunities, not salary...zone when you lose track of time because you are engrossed in a task. Things seem to fall into place frequently... -
Viral Vectors 101: Gamma-Retroviral Packaging Systems
TypeBlog PostPublishedAug. 6, 2024, 1:15 p.m....asked questions about γ-retroviral vectors How do engineered γ-retroviruses differ from lentiviruses? There...There are a few important differences between engineered γ-retroviruses and lentiviruses. First, these viruses... -
Clear and Accessible Writing
TypeBlog PostPublishedFeb. 15, 2024, 2:15 p.m....scientific paper, you may feel it’s overwhelming or challenging. So instead, let’s focus on practicing with a...dive into applying these four steps elsewhere, engaging in this practice, and perhaps modifying it as ... -
CasPEDIA: A Functional Classification of Cas Enzymes
TypeBlog PostPublishedJan. 25, 2024, 2:15 p.m....can I use it? CasPEDIA operates like many search engines, featuring both a general search bar tool and an... CRISPRpedia IGI’s digital resources in English/Spanish ... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog PostPublishedApril 27, 2023, 1:15 p.m....Campus and the University of California, Berkeley engineered an AAV variant that efficiently infects neurons...recombinase-dependent vectors, this can cause challenges for the experimental design as these vectors ...