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We narrowed to 924 results for: Gene

Showing: 381 - 400 of 924 results

  1. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    Published
    May 15, 2018, 12:43 p.m.
    ... to the Hot Plasmids episode! Expanding the optogenetics toolbox with CRY2clust Article contributed by...Basic Science (IBS) has developed another useful optogenetic tool that enables robust and efficient oligomerization...CRY2clust, when integrated into a Ca2+ -modulating optogenetic system (OptoSTIM1), caused two-fold faster changes...soCoChR: A novel opsin that enables single-cell optogenetics Article contributed by Meghan Rego  Listen ...Listen to the soCoChR podcast segment Optogenetic stimulation is a powerful tool used by neuroscientists to...promoters and/or fluorescent tags are available at Addgene. Shemesh OA, et. al. Nature Neuroscience. 2017...The design use to detect PKA activity can be generalized to measure phosphatase activity, second messenger...

  2. Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies

    Type
    Blog Post
    Published
    Feb. 26, 2019, 1:40 p.m.
    ...opportunity. That's how Addgene was founded. That's how my position and my team at Addgene was created.”- Eric...discussed on the Addgene blog, consider writing a guest post. Additional resources on the Addgene blog Read...transferable skills guide Resources on Addgene.org Find job listings at Addgene Get more career insight from Addgenies...  We touch upon these topics frequently in the Addgene blog. In fact, we have so many blog posts about...into one comprehensive, downloadable guide: the Addgene Science Career Guide. We also asked Addgenies what...

  3. Addgene’s Newest Way to Speed Your Research: The Cloning Grade DNA Service

    Type
    Blog Post
    Published
    July 28, 2020, 1:15 p.m.
    ...experiment? What if after receiving the plasmid from Addgene you could directly start your digest, PCR, or transformation...preparation is quality controlled through next generation sequencing and spectrophotometry to test purity...Suggestion Form.  Additional resources on the Addgene blog Browse blog posts from our Plasmids 101 blog...the various ways to quantify DNA Resources on Addgene.org Learn about our other beta projects Read our...

  4. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ...the antibiotic resistance gene and origin will replicate, and the transgene will be lost. Over time, bacteria...vector backbone contains the antibiotic resistance gene (AmpR) and origins of replication (ori and f1 ori...these cloning strains, browse the table of common gene mutations in our Plasmids 101: Common Lab E. coli...Working on the scientific support team at Addgene, I get to see everything that can go wrong with a plasmid...recombination, one resulting plasmid carries the transgene of interest (aka the viral genome) and the other...10.1186/s12934-020-01321-4   Kolodner R (1980) Genetic recombination of bacterial plasmid DNA: electron...10.1261/rna.069286.118 Additional resources on the Addgene blog Find more molecular biology and viral vector...

  5. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type
    Blog Post
    Published
    May 25, 2021, 2:29 p.m.
    ...2018), and hybridomas can lose gene expression of the mAb-encoding genes, die, or fail to revive after ...broadly, advances in gene synthesis, affinity reagent screening, and next-generation sequencing of hybridomas...grants and Addgene’s mission This is the first NIH grant that Addgene has received. Addgene prides itself...allow Addgene to further our mission of scientific sharing,” says Dr. Daniela Bourges, Addgene’s interim...opened the door for a new generation of recombinant affinity reagents. Addgene is looking forward to working...antibodies Resources on Addgene.org Find recombinant antibody plasmids at Addgene ...to improve upon these critical research tools. Addgene is excited to announce that we have recently received...

  6. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...targeting a gene associated with diphtheria toxin lethality and were sorted into single wells to generate clonal...instance, it’s much more difficult to introduce a gene of interest into primary cells, so most primary ... risk of viral integration causing mutations and gene expression changes elsewhere in the genome. Second...j.cell.2015.03.028. PubMed. Find the Plasmids @Addgene: iTOP-tested proteins and reporters Previous...

  7. Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering

    Type
    Blog Post
    Published
    Nov. 12, 2014, 3:54 p.m.
    ...simultaneous activation and/or repression of multiple genes, and more. The researchers now plan to adapt the...powerful tools to modify the expression of multiple genes at the same time.” For more details on how to apply...the Mulitplex CRISPR/Cas9 Assembly System Kit at Addgene here. Other Popular CRISPR Posts: How to Design...

  8. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

    Type
    Blog Post
    Published
    July 18, 2017, 12:41 p.m.
    ...in applying gene technology to bacteria and plants. Her research involves cloning cry genes from the soil... shared mission of both Seeding Labs and Addgene. The Addgene-Seeding Labs plasmid grant is a collaborative...of the Addgene-Seeding Labs Plasmid Grant awardees' projects progress. Subscribe to the Addgene blog for.... Additional Resources on the Addgene Blog Learn More About the Addgene-Seeding Labs Plasmid Grant Check...introduce the 5 scientists who were awarded the Addgene-Seeding Labs Plasmid grant this year. These scientists...plant biology, she coordinates the Network for the Genetic Improvement of Cowpea, a knowledge database for...potential agricultural and medical use. Find More Optogenetic Tools Like pDawn  Making cassava plants resistant...

  9. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type
    Blog Post
    Published
    May 19, 2020, 1:15 p.m.
    ...protein coding genes in E. coli and S. cerevisiae expression vectors are available at Addgene. A set of mammalian...yeast expression constructs contain protein coding genes, tags, truncations, and codon optimizations similar...of plasmids containing SARS-CoV-2 protein coding genes in E. coli and S. cerevisiae expression vectors ... available at Addgene Read about SARS-CoV-2 detection methods Resources on Addgene.org Find all plasmids...been functionally tested by Ginkgo Bioworks or Addgene. If you used plasmids from this collection in your...COVID-19 Plasmid Collection Quality control at Addgene in the midst of COVID-19 We received Ginkgo’s first... our standard full plasmid sequencing by NGS. Addgene’s QC scientists immediately screen these plasmids...

  10. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type
    Blog Post
    Published
    Oct. 30, 2018, 1:04 p.m.
    ...expression of four embryonic genes- OCT4, SOX2, KLF4, and C-MYC. iPSCs were first generated from mouse fibroblasts...differentiate into specialized cell types [2]. We general speak of 4 levels of stem cell potency: Totipotency... in many organs in the adult human body. These generate specialized cells for their location, mostly to...isolated from the human body Usage Universal. Can generate all adult cell types. Limited. Only specific cell... realistic options for scientists working in regenerative medicine, but the longing for a model system...Yamanaka and Thomson labs showed the successful generation of human iPSCs from dermal fibroblasts via retroviral...Prize for Dr. Yamanaka in 2012. Find plasmids for generating iPSCs iPSCs and ESCs closely resemble each other...

  11. Hot Plasmids Podcast Episode 3: Optogenetics Tools, Improved CRISPR Base Editors, & More!

    Type
    Blog Post
    Published
    Oct. 25, 2017, 1 p.m.
    ...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional... Plasmids podcast series, you'll learn about optogenetics tools for controlling protein activity, methods...Base Editors Hot Plasmid 4: pPSU Plasmids for generating DNA molecular weight markers.  Listen to the ...Additional Resources on the Addgene Blog Listen to Our Podcast Interview with Eye Researcher Connie Cepko...Science Videos from Tom McFadden Resources at Addgene.org Sign up to Receive Our Newsletter Check Out ... optogenetics...

  12. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...for the generation of one or more mutations – it is as easy as obtaining a plasmid with the gene of interest...interest (search Addgene plasmids by gene here), designing primers with your desired mutation, performing... desired promoter, selectable marker, and target gene, for example, are amplified with overlapping regions... E.coli for plating, screening and generation of sgRNA. Generation of sgRNA libraries, with maximum complexity...multiple mutations in one or multiple fragments, and generate constructs for producing single-guide RNAs – but...the NEBuilder HiFi DNA Assembly Mix include:  Generation of constructs with multiple point mutations Cloning...sgRNAs Production of Yeast Expression Cassettes Generating constructs with multiple point mutations NEBuilder...

  13. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...inhibition library that inhibits over 18,000 human genes and is used for genome-wide inhibition screening...types, recombinases allow you to further restrict gene expression to areas in which the recombinase and... patterns, thus allowing you to further restrict gene activation to cells expressing both Cre and GFP....pAAV-hDlx-Flex-GFP-Fishell_6 Gordon Fishell   Optogenetics Optogenetics continues to supply neuroscience with ...mCherry-Fishell-3 Gordon Fishell   Chemogenetics Chemogenetics offers noninvasive methods for manipulating...help@addgene.org with the vector name and serotype you’d like to be notified about. Chemogenetics Plasmid...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...

  14. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ... nucleic acid sequence of the antibody-encoding genes over time as the hybridoma cells divide. These changes...before culturing or they can clone the genes from the hybridoma into a plasmid to create a recombinant...Figure 2: Changes to the heavy chain or light chain genes within a hybridoma impacts the specificity, avidity...Resource on Addgene.org Check out Addgene’s antibody plasmid collection Read the latest on Addgene's new antibody...above, genetic drift is one concern when using hybridomas to produce monoclonal antibodies. Genetic drift...the target molecule. Although it’s possible to generate antibodies against many types of molecules including...recognize proteins. Figure 1: Steps in the generation of hybridomas for monoclonal antibody production...

  15. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    Published
    Nov. 27, 2018, 2 p.m.
    ...her current research, Sheen aims to identify new genes related to PZA-resistance by using different approaches...as pACBSR-hyg and pFLP-hyg will be used to create gene deletions of important unknown regulatory regions...growth will then be measured to link the deleted genes to specific functions in biofilm formation. The...on the Addgene Blog Learn More About the Addgene-Seeding Labs Plasmid Grant Check Out Addgene-Seeding...of the Addgene-Seeding Labs Plasmid Grant awardees' projects progress. Subscribe to the Addgene blog for...Susanna Bachle and Ina Ersing Seeding Labs and Addgene collaborate to provide scientific resources, equipment...resource-limited settings. More specifically, the Addgene-Seeding Labs plasmid grant provide plasmids to ...

  16. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type
    Blog Post
    Published
    Sept. 18, 2018, 1:33 p.m.
    ...exhibiting its versatile utility in quantifying multiple gene editing activities. Click here to find the ACE ...Wanisch  Listen to the optogenetics podcast segment LOVTRAP is an optogenetic system that was recently... the University of North Carolina (plasmids at Addgene). It consists of two domains, LOV2 and Zdk1, where...function is more specific and much faster than any genetic or pharmacological manipulations on cells, and ... riboswitches used in the Riboglow platform at Addgene. Braselmann E, et al. Nature Chemical Biology...

  17. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

    Type
    Blog Post
    Published
    July 2, 2015, 8:37 p.m.
    ... we can make Addgene better and make scientists’ lives easier. Why transition to Addgene? So why did ...more satisfying, because Addgene is a non-profit, we can focus more on generating blog content that scientists... that PhD. Resources at Addgene Read Other Careers Posts Visit the Addgene Careers Page Learn about ...Harvard University and entered a new role here at Addgene as an Outreach Scientist. I used to spend my days... less time wrangling bacteria and instead help Addgene fulfill its mission of helping scientists share...did I join Addgene? Was I jaded by my time in academia? Did I run screaming from the lab like the cartoon...didn’t, it would have been unwise of me to join Addgene. This is particularly true in my position as an...

  18. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...domain due to a gene that resembles cas10. All type IV subtypes are missing adaptation genes, Cas1 and Cas2...sequence similarity, phylogenetic analysis, neighborhood analysis of surrounding genes, and experimental ...straightforward as it seems. There is not a single gene that is shared across all CRISPR types; the fast...together because they contain a distinct Cas7-type gene. The exact mechanism of type IV systems is not known... and applications with the type I CRISPR system. Gene and Genome Editing, 3–4, 100013. https://doi.org...the array of CRISPR technologies. Learning the general principles of this classification system will help...A–C, with the beloved SpCas9 (from Streptococcus pyogenes) belonging in II-A. Cas9 was the first to be adapted...

  19. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...’s first genetic engineering company, Genentech, was founded.  This commercialization of gene editing ...before the discovery of genes themselves.  How has scientific progress in genetic editing impacted the world... DNA sequences is not ideal. For the early generation gene editors, new amino acid recognition sites had...followed by non-homologous end-joining that results in gene knockouts.   Star Trek: Into Darkness (2013) which...The major drawback of all the endonuclease-based gene editors (meganucleases, ZFNs, TALENs, and CRISPR-Cas...Gregor Mendel on the genetic inheritance (1866) and just when the Mendelian genetics was being revived by...Zinc-Finger Nucleases. Genetics 188:773–782 . https://doi.org/10.1534/genetics.111.131433 Doudna JA, Charpentier...

  20. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

    Type
    Blog Post
    Published
    Nov. 20, 2018, 8:17 p.m.
    ...URL “http://n2t.net/addgene:87155” where the Addgene registered prefix and the Addgene ID # are directly...Daniel Bauer (Addgene plasmid # 87155 ; http://n2t.net/addgene:87155 ; RRID:Addgene_87155).” So when citing...Daniel Bauer (Addgene plasmid # 87155 ; http://n2t.net/addgene:87155 ; RRID:Addgene_87155)” Cite plasmid...through Addgene and find verified information about each plasmid item. Given a specific Addgene ID # listed...available through Addgene. Let’s take a look at lenti-Cas9-VQR-Blast, which has the Addgene ID #87155. The... as “RRID:Addgene_87155” and the following URL (https://scicrunch.org/resolver/RRID:Addgene_87155) will...contain the Addgene ID # and the information necessary to indicate that this ID is managed by Addgene. How to...
Showing: 381 - 400 of 924 results