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We narrowed to 878 results for: IND

Showing: 381 - 400 of 878 results

  1. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ...able to find strategies and systems that set you up for success in the detail-driven daily grind of science...tedious, but it also works, so do it anyway.  You may find that after running a protocol a few times, you no... blot protocol, from a full page to a few key reminders on a sticky note   Visual Planning  Plate ... use cold block systems or buckets of ice. Try finding ways to intentionally set up your bench to help...forth, continuing all day or even all week. By finding ways to intentionally slow down, you’re giving ...

  2. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    Published
    June 25, 2019, 12:54 p.m.
    ... passed down through cell division. You can find all kinds of different lentiviral and AAV plasmids as...protocols for plasmid cloning Find our molecular biology reference here Find molecular biology protocols... of a fine needle to deliver nucleic acids to individual cells. Electroporation on the other hand uses...edition (2000). Meibom, Karin L., et al. "Chitin induces natural competence in Vibrio cholerae." Science...

  3. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    Published
    June 6, 2024, 1:50 p.m.
    ...synthetic beads that can bind to a conjugated antibody and beads that are unable to bind to them: like cells...real results from background noise or nonspecific binding. Flow specific controls The three types of flow...they are non-specific. The compensation beads can bind to any primary antibody you add. Single color controls...allow you to determine what level of non-specific binding you have in your sample. The isotype antibody will... This enables you to identify any non-specific binding to know that your positive cells are truly positive...

  4. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    Published
    July 6, 2017, 1:59 p.m.
    ... and Immunity and learn more about “... recent findings in basic immunology, cancer-immune cell interactions...discussed the effects of starvation on cancer therapy. Findings in previous animal studies suggested that fasting...in trying to resolve the inflammatory state by inducing immunosuppressive measures, for example the upregulation...impressive videos of these interactions (Ref 3). You can find useful tips on in vivo imaging in our Fluorescent...scientific community, contact [email protected] to find out more about our free deposit and distribution...Browse Cancer Plasmids Browse Immunology Plasmids Find Kits for Cancer Research  ...

  5. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...of interest, as it is prone to generating indel errors. Indel errors generated in the course of repair ...where mutations are introduced. Repair of Cas9-induced breaks by NHEJ As illustrated below, NHEJ-mediated...microhomologies”). NHEJ doesn’t obligatorily introduce indels. Given the end structure of the Cas9 DSB (blunt... of accurate repair are easily re-cleaved while indel products aren’t, so repeated cycles will favor accumulation...constitutively expressing a targeted Cas9 should possess indels in the majority of their chromosomes within a day...

  6. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ... at multiple levels to generate anything from individual transcription units (TUs) used to express your...metabolic pathways. For many MoClo systems, the individual parts are reusable, saving time and money on ...some common MoClo applications and kits. Keep in mind that this technology is useful for a variety of ...purification tags, connectors, cDNAs, and more to find the perfect combination. The EcoFlex Kit has a wide...array of DNA parts that can be assembled into individual TUs or very large (up to 20!) multi-TU constructs...et al., 2016). The variety of constitutive and inducible promoters, linkers, and tags makes EcoFlex a great...kits are designed with specific model organisms in mind, others are designed for flexibility. The Fragmid...

  7. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...system, antibodies are produced by B cells. Each individual B cell produces antibodies that all recognize...purification is one common method. These proteins bind the IgG heavy chain. Running the serum over a column...column of resin crosslinked to protein A /G will bind the IgG antibodies present in the serum, whether... are isolated from the serum by their ability to bind the target antigen. In this case, the serum is run...antibody; the ability of polyclonal antibodies to bind many epitopes of their target protein thereby greatly...polyclonal isotype control, to account for nonspecific binding. Now that you understand the pros and cons of choosing...

  8. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type
    Blog Post
    Published
    May 11, 2021, 1:15 p.m.
    ...2013). We found that the fluorescent proteins can, indeed, interfere with the correct function of the protein... species (bottom panel in Fig. 1). Our results indicate that the tag protein may have an important influence...experiment. Since the fused protein could represent a hindrance to the successful expression of the protein of...opsin-expressing cells). For other things to keep in mind when making fluorescent protein fusions, check out... posts Read about chemogenetics vs. optogenetics Find more about the many types of protein tags References...Addgene.org Check out Addgene's Chemogenetics Guide Find fluorescent proteins at Addgene ...

  9. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ...established, it’s also a process that’s difficult for individual labs to complete. The nanobody based RANbody ...purification. Antibodies are difficult for an individual lab to produce - Hybridomas offer an alternative...RFP,  the histone protein H2A2B, and the actin-binding protein gelsolin, but any nanobody sequence could...serves as a backup means of detection that’s independent of the reporter. The only caveat is that HA staining.... Staining with multiple HRP-RANbodies is indistinguishable since they produce the same signal, so only...plasmids from Yamagata et al can be found here. Can’t find a RANbody against your favorite protein? No worries...

  10. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ...qualify what kind of “plate” you mean. Without context, it’s difficult to know what kind of plate they...figure out the sources of miscommunication and b) find practical solutions.  My PhD prepared me remarkably...document, I update the training frequently. In hindsight, getting a list of frequently used words like ...Addgene blog Read about other transferable skills Find all of our science career related blog posts Read...communication blog posts Resources on Addgene.org Find careers at Addgene ...

  11. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ...next week!”, and “This is his job!” filled my naive mind. Moving forward from that first experience, I learned...process, she has faith in you! Additionally, keep in mind that universities require application submission...the mission of the agency and benefits the field reminds the reviewer why your project is the project to...travel, etc. As a first time budget writer, you may find this section challenging because it’s hard to determine...students and postdocs will be hired, direct and indirect costs, etc. It may be helpful to ask your advisor...application process? What types of writing feedback do you find most helpful? Have a great grant writing tip? Share...

  12. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    Published
    Dec. 14, 2021, 2:15 p.m.
    ...domains tethered to dCas9 often interact with DNA independently of dCas9 targeting (Galonska 2018). This means...importantly, TET proteins affect transcription independent of their enzymatic activity. They interact with...straightforward as expected. TET1 can have many effects independent of dCas9 targeting, including modifying other... This may seem counterintuitive: how can a DNA-binding protein with no enzymatic activity cause demethylation...are key for complete DNA demethylation – tight binding is crucial for dCas9 to interfere with DNMT1 even...02708-5 Hrit J, Goodrich L, Li C, et al. (2018) OGT binds a conserved C-terminal domain of TET1 to regulate...

  13. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    Published
    June 26, 2018, 1:33 p.m.
    ... However, the molecular and cellular mechanisms behind the immune defects in CDG patients are still poorly...Arg) amino acids more frequently than any other. Indeed, nearly 20% of the single missense mutant alleles...could improve health outcomes for millions of individuals worldwide.Arg is unique among amino acids in ...Rescue occurs through guanidinium cation (Gdm+) binding in the location formerly occupied by the Arg sidechain...) because, at high concentrations (25 mM), Gdm+ binds to and inhibits a potassium channel, causing release...: Baldwin, Enoch, et al. "Generation of ligand binding sites in T4 lysozyme by deficiency-creating substitutions...

  14. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...using FRET! Read on to find out more about this amazing imaging technique and find further tips for using...or the activity of a protein in a synapse. What kinds of tools enable us to measure these properties, ...neuronal activity (voltage sensors), to name a few.   Find Many FRET-Based Biosensors on Addgene's Biosensor...PMC18018. 2. Miyawaki, Atsushi, et al. "Fluorescent indicators for Ca2+ based on green fluorescent proteins ...Biosensors Additional Resources on Addgene.org Find FRET Pairs Check Out Fluorescent Protein Biosensors...

  15. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...our Hot Plasmids articles.  Here's what you'll find in this post: Peptide-assisted genome editing for...Adapted from Zhang et al. 2023 with permission.   Find Cas-PAGE plasmids here!   Zhang, Z., et al. (2023...on mStayGold inspired and informed this section.  Find mStayGold plasmids at Addgene here!   Ando, R.,... Goeden, et al. 2023, under CC-BY license.   Find the AAV.CAP-Mac plasmid here!   Chuapoco, M.R.,...from Chen et al (2023) under CC-BY license.   Find AAV1-X1 plasmid here! Chen, X., et al. (2023). Functional...has been widely used in a variety of applications. Find anti-DYKDDDDK recombinant antibodies here!...

  16. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...yourself: is it clear how novel my findings will be and why these findings will be worth knowing? This can...tricky, as most of our research builds on ideas and findings from our own or other groups, but your work must...to know what we are doing and why. I always ask kindly friends and family to read my non-expert paragraph...back at my successful applications, I can't always find something that made them better than my unsuccessful...up an ELISA kit company on whose website you can find some great information on ELISA troubleshooting ...

  17. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...SSRs bind DNA at target sites to induce site specific recombination events: Cre recombinase binds loxP...the Cre-loxP recombination system can be used to induce site-specific recombination events, and that the...loxP sites, while FLP binds FRT sites. When a DNA sequence is flanked by target sites (floxed) in opposing...PMID: 2286602. PubMed Central PMCID: PMC3406316. Find FLEx plasmids from these Addgene depositors: Ed...

  18. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ...site selection by TnsD, a sequence-specific DNA binding protein. Selection removes donor bacteria and recipient...subtilis to the bacterium of interest following IPTG induction of conjugation genes and bi-parental mating. Following...and colleagues used Mobile-CRISPRi to knockdown individual genes in several bacteria associated with human...measured after 6 and 12 generations with or without induction of CRISPRi knockdown. As expected, bacteria expressing...move Mobile-CRISPRi into your bacteria of interest? Find Mobile-CRISPRi plasmids at Addgene! References...Addgene.org Browse our CRISPR interference plasmids Find bacterial CRISPR plasmids Read our CRISPR guide ...

  19. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...traditional restriction cloning. Sequence and Ligation Independent Cloning (SLIC) could also be a great choice, ...non-recombinant vectors from propagating. The ligation-independent methods like Gibson and SLIC are also quite efficient...you some tips for your next project. But keep in mind that there are many other cloning methods - including...don’t be afraid to explore multiple approaches to find out which one works best for you. Happy cloning ...simplified, purification-free, sequence-and ligation-independent PCR cloning method." BMC biotechnology 11.1 (...Addgene.org0 Check out protocols for plasmid cloning Find vector backbones Read our molecular biology reference...

  20. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ... and an extension sequence containing a primer binding site and a reverse transcription template (RTT)...high-throughput libraries My labmates and I wanted to find a way to predict the efficiencies of pegRNA designs...the RTT overhang, the GC content of the primer binding site, and many more. Once we had our datasets with...experiments Prediction models only become useful once they find their way into the hands of users. For this reason...resources References Akhtar, W., De Jong, J., Pindyurin, A. V., Pagie, L., Meuleman, W., De Ridder, J....B. (2021). Impact of chromatin context on Cas9-induced DNA double-strand break repair pathway balance....
Showing: 381 - 400 of 878 results