We narrowed to 784 results for: IRE

...are under a Creative Commons 3.0 license, which requires users to give appropriate credit, provide a link...indicate if changes were made to the images. This requirement means the images are probably better suited for... image search is a great tool for searching the entire internet for scientific graphics. The tools settings...

... the main source of the body’s DA. Neurons often fire at a certain frequency, and this frequency can indicate...frequency of its release) are well understood, the direct effect of DA binding to a target neuron is more...the region of the brain where DA quantitation is desired (Figure 1). The probe perfuses liquid, which causes...

...    Novel fluorescent enzyme biosensors provide direct, specific and sensitive detection of the plant ...strigolactones (SLs) are costly, non quantitative or require heavy optimization. Claudia Vickers’s lab has designed...than on the fluorescence intensity, it provides a direct and quantitative detection of SLs. The authors ...

...transgene (Lin et al., 2018). However, this system requires precise titration of the AAV. So far, tools to...to overcome this challenge in Drosophila require heat-shocking the system or using chemical inducers of... that will be labeled in a given population is directly correlated to the length of the truncated attP...

...many roles to play inside, on the outskirts, and entirely outside of academia. These roles can include positions...position you’ll be happy in once you’ve finally acquired that PhD. Resources at Addgene Read Other Careers...Management for Scienitsts from Addgene Executive Director Joanne Kamens ...

...does not use restriction enzymes or ligase, and requires no post-PCR procedures. Sounds easy right? The... proofreading polymerases will also remove all unpaired 3’ ends in your PCR product. If you need to decrease...Additional Resources on the Addgene Blog Perform Site Directed Mutagenesis by PCR Use REPLACR Mutatgenesis to...

... Growing Arabidopsis in the classroom does not require a huge investment in resources. The materials needed...guideline, experiments that include growing plants require pots, trays, soil, fertilizer, seeds and a growth...space with fluorescent lights. Other experiments require only seeds, petri dishes, and filter paper. Another...

... Using structural modeling and structure-guided direct evolution, Nathan Shaner and colleagues from The...violet range. For example, mWasabi can be used with Sapphire (excitation maximum 399 nm) because it does not... at Addgene! References Ai, Hui-wang, et al. "Directed evolution of a monomeric, bright and photostable...

...in the last few months. You can also browse our entire AAV inventory. Our new AAVs include: Optogenetics... See our Serotype Testing AAV inventory for the entire collection! Plasmid Serotype Name 51502 AAV5... sensors provide the spatiotemporal resolution required for precise in vivo measurements of dopamine (...

...cells by any means, but are unique in that they require delivery to be viral-mediated. Transformations ...retrovirus, lentivirus, adenovirus, or AAV. Then, your desired DNA to be integrated can be cloned into the vector...weeks, depending on the virus, cell type, and/or desired event.   Figure 1: Transduction to expression...

...operations for all ongoing processes are entirely self-supporting. We hire more staff as we need them to meet... were closed and not receiving packages. So the entire team mobilized to figure out how to deliver when...

...rate discovery rate, conventional CRISPR screens require a low multiplicity of infection (MOI) during cell... tools for doing this were labor-intensive and required using manipulations such as injection of precise...drives gene expression. Because calcineurin is required for virulence in pathogenic fungi, it is an attractive...

...using AAV on our blog. Image: The final and required step requires submission of an experimental image result...expression of AAV cargo? Did you use GCaMP expression? Direct Fluorescence? Immunohistochemistry? Did you use...

...rare diseases,” said Claudia OuYang, BeHEARD Co-Director in a release. The research teams studying four...says that the lab got interested in XP out of a desire to understand the early events in the transformation...variants and strategies out there. This will help us acquire a lot of those plasmids so that we can try many...

...Being an entrepreneur is difficult; it requires an immense amount of self-knowledge and an unwavering...  To start, the average health science startup requires a great deal more capital to get off the ground... your name (P-H-D).  Finally, having spent your entire career in science, you are likely lacking the necessary...

...Addgene Blog Mentoring for Scientists Series. The entire series and additional resources can be downloaded...responsibility of arranging meetings and setting the direction in a mentoring relationship falls on the shoulders...Mentoring for Scientists" ebook, which includes the entire series of mentoring blog posts, the "Forced Choice...

...communication. A great approach to fully understand and acquire competitive skill sets in a myriad of professions...job sites to find open positions and view their required qualifications and skill sets Taking the time...graduate students and postdocs are currently getting hired? Let us know what topic you'd like to learn more...

...scope of the meeting: Are you meeting to design an entire experiment and delegate tasks to lab mates or to... to discuss a potential new research direction you’d like to pursue? Undefined scope can lead to long ... Get Management Advice from Addgene Executive Director Joanne Kamens FInd Tips on Picking a Research ...

.... The tools are a lifesaver when your research requires cell culture and include a counter, lab timer,...Tools app comes in handy when I use protocols that require restriction enzymes or PCR. The app contains a ...the app is quite helpful and provides a list of directions including which buffers to use and that indicates...

...BosLab and previous laboratory experience is not required. Our members are skilled with audiovisual media...initiated a soil microbiome project. This project was inspired by previous city-wide genomic studies and our ...DIY bio If you’re not in the Boston area but are inspired to get involved, check out the local groups page...