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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 791 results for: Kin

Showing: 381 - 400 of 791 results

  1. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    Published
    Oct. 7, 2014, 3 p.m.
    ...because it finally feels to me like the postdocs are taking some steps to control their own future in science...speaker, Dr. Henry Bourne (shown in photo), has been speaking out and writing about issues in our science training...experience and encouragement.  Dr. Sibby Anderson-Thompkins, Director of the Office of Postdoctoral Affairs...

  2. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    Published
    Aug. 13, 2014, 3:49 p.m.
    ...Streptococcus and the “py” in pyogenes), capable of locking two peptide tags together. SpyLigase overcomes ...forces acting on them. For the application we were looking at, magnetic isolation of circulating tumor cells..., highly stable and has this amazing chemistry locking the domain together - no one knows what for. Many...

  3. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ... no fear - plasmids from Addgene depositors are making it easier than ever to use CRISPR  in E. coli and...gene body can repress transcription by physically blocking the elongation complex from binding the DNA or...Patino R, Heussler GE, Hearne CC, Qu J, Inclan YF, Hawkins JS, Lu CHS, Silvis MR, Harden MM, Osadnik H, Peters... Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA (2013) Repurposing CRISPR as an RNA-Guided...

  4. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ...Especially when your collaboration includes scientists working in different time zones, it’s important that everyone...accountable to these expectations once they’re set by checking in about them at your recurring meetings and reaching...common. I hope these tips are useful whether you’re looking to improve communication within an existing collaboration...

  5. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...fluorescent signal and enables single molecule tracking  within living cells without affecting protein...managed to track run lengths of the motor protein kinesin across microtubules. Tanenbaum et al. then tested...photobleaching Simplified, long-term single-molecule tracking Caveats of SunTag Very large size: a 24x scaffold...

  6. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    Published
    June 29, 2023, 7 p.m.
    ...video. Don't worry - no pipettes were harmed in the making of this photo!  Here, I was also given a mixture...such as stuffing new depositor welcome kits, note taking for protocol video production, and keeping inventory... my time here comes to an end, my worries about making connections with coworkers seem, in retrospect,...

  7. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    Published
    Sept. 12, 2023, 1:15 p.m.
    ...mapping These tools are for viewing, editing or making plasmid maps, but can also analyze and annotate... Viewer: The free SnapGene Viewer is great for looking at plasmid maps and viewing sequencing traces, ... blank spaces, from the text of a DNA sequence, making it ready to copy and paste into other applications...

  8. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    Published
    Nov. 22, 2016, 3:30 p.m.
    ...headquarters. I decided to go ahead and apply; thinking I probably would not get selected for both the...compose and submit a manuscript that they were working on currently. Upon arrival to the headquarters,... The most beneficial aspect of this course was breaking up into focus groups with a facilitator. In the...

  9. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    Published
    Aug. 22, 2019, 12:43 p.m.
    ... cells containing the plasmid construct you’re seeking. Fortunately, there are many ways to do this involving... a successful uptake of your DNA of interest by taking advantage of bacterial characteristics such as ...for specific amino acids. When plated on media lacking the essential amino acid, the bacteria will only...

  10. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...also involved in reinforcement learning, decision making, and motor control.  What do we understand about... 100 nm) of DA. In addition, they exhibit fast kinetics (10 ms on and 100 ms off), enabling detection ...magnitudes of fluorescent responses. Studies on the kinetics of these sensors showed rapid response (60 or ...

  11. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...background noise. This is not terribly problematic when working with cell culture, because it is easy to harvest...harvest large quantities of cells. However, when working on specific tissues or organs in whole animal models...major technical hurdle.   ChIP-seq requires  cross-linking during an initial fixation step. This serves to...

  12. Oh, The Places You Can Go: Careers in Science Communication - Product Development

    Type
    Blog Post
    Published
    Oct. 18, 2017, 1 p.m.
    ...bunch. They don’t really know where I came from.” Working at eScience Labs, an educational company that creates...that provides scientific editing services. “I was working during the day and editing at night. It was not...moved out here, I was still editing from home and looking for a job.” But Caitlin’s persistence paid off:...

  13. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...expressing a selection cassette with 5′ and 3′ flanking sequences matching the sequences upstream and ...to be assayed (e.g. pPD95_86 and pPD57_56), or knocking down YGOI through RNA-mediated interference (e.g... applications used to study gene function. From making transgenic animals, gene targeting and genome modifications...

  14. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ... used at high concentrations (and vice versa). Looking around the lab, you'll likely find many of the ...mammalian cells.   Name Class Mode of Action*   Working Concentration** Kanamycin aminoglycoside Binds...their mechanism for killing bacteria, and general working concentrations. For instructions on how to prepare...

  15. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ...replication machinery room to come in and get busy making copies.     Types of origins of replication ...compatibility with one or more other plasmids. Generally speaking, plasmids with the same ori are incompatible because...chloramphenicol – make sure your growth conditions aren't working against you!  The culture inoculum Freshly ...

  16. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...Addgene started dealing with this new reality of working remotely, scientists around the world mobilized...took to make all this happen with 80% of the team working remotely. We’re extremely proud of the work we’... maintain our workforce for when we need to be working at full capacity to serve the scientific community...

  18. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ...around the world who would otherwise have difficulty making or getting these reagents is profound.” Frank Stegmeier...Library, a library that “enables the high-resolution tracking of more than 1 million cancer cells under drug...became passionate about virology,” he says. He was working in an HIV lab and based on that experience, soon...

  19. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...frequently with little notice or choice Always working on a team/in a matrix - can be frustrating for ...but good performers move around easily  Table 1: Taking it a Step Further - Big Company v. Small Company...on what paths to pursue. Spend some time really thinking about your motivations and what parts of being...
Showing: 381 - 400 of 791 results