We narrowed to 405 results for: Lif;
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TypeBlog PostPublishedApril 8, 2025, 1:15 p.m....blot, and depending too much on normalization can amplify other errors in experimental design and gel quantification...
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CRISPR 101: Homology Directed Repair
TypeBlog PostPublishedJan. 26, 2023, 2:15 p.m.... a broken relationship. Pardo B, et al. Cell Mol Life Sci (2009). PubMed. Regulation of DNA double-strand... -
Transferable Skills Guide: Conflict Resolution
TypeBlog PostPublishedJan. 4, 2022, 2:15 p.m.... be the most controversial, so I would like to qualify it. If you are attempting to resolve conflicts ... -
Antibodies 101: Introduction to Immunofluorescence
TypeBlog PostPublishedNov. 16, 2021, 2:15 p.m....bind to a single primary, resulting in signal amplification. Another reason is cost effectiveness. Generating... -
10 Steps to a Perfect Science Talk
TypeBlog PostPublishedAug. 23, 2016, 2:30 p.m....talks We are not talking about lab meeting here. Lifeless presentation of experimental results in the order... -
Evolution of Lab Techniques
TypeBlog PostPublishedJune 21, 2016, 2:30 p.m....interest by recording DNA abundance throughout the amplification process, rather than just at the end as in standard... -
Treating Muscular Dystrophy with CRISPR Gene Editing
TypeBlog PostPublishedJan. 26, 2016, 3:30 p.m.... last, and more importantly, if it does extend lifespan. This work should be done in mouse and large animal... -
Plasmids 101: Common Lab E. coli Strains
TypeBlog PostPublishedNov. 7, 2014, 2:56 p.m....vectors. Used by Addgene for pooled library amplification. F' mcrA Δ(mcrBC-hsdRMS-mrr) recA1 endA1 gyrA96... -
Easi-CRISPR: Generating Knock-In and Conditional Mouse Models
TypeBlog PostPublishedApril 5, 2018, 12:42 p.m....feature (e.g., a fluorescent protein), you can amplify the desired region using primers containing the... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog PostPublishedJan. 16, 2025, 2:15 p.m....Arandel, L., Marhic, A., Chapart, M., Vasseur, S., Benkhelifa-Ziyyat, S., Bigot, A., Butler-Browne, G., Mouly... -
Viral Vectors 101: Optogenetic Tools
TypeBlog PostPublishedJune 27, 2023, 1:15 p.m.... pathways. Additionally, as GPCR signaling is amplified in successive steps along a pathway, there can... -
Antibodies 101: The Basics of Western Blotting
TypeBlog PostPublishedMarch 8, 2021, 2:41 p.m....antibody molecules bind the primary antibody and amplify the detection signal. Analyzing the western blot... -
PiggyBac-ing Through the Genome Editing Field
TypeBlog PostPublishedMay 31, 2016, 3:30 p.m....between inverted loxP sites is cytotoxic for proliferating cells and provides a simple tool for conditional... -
9 tips for a successful postdoctoral experience
TypeBlog PostPublishedOct. 9, 2018, 12:21 p.m....yourself, and to address all of the other parts of your life, which, inconveniently, do not take time off when... -
Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering
TypeBlog PostPublishedDec. 15, 2016, 3:57 p.m.... inserts can be made by PCR using primers that amplify the DNA sequence of interest and flank it with ... -
RNA Interference in Plant Biology: New Tools for an Old Favorite
TypeBlog PostPublishedOct. 27, 2020, 1:15 p.m....Artificial MicroRNAs for Multiplex Gene Silencing and Simplified Transgenic Screen. Plant Physiol 178:989–1001... -
Antibodies 101: Antibody Engineering and Directed Evolution
TypeBlog PostPublishedAug. 5, 2025, 1:15 p.m.... goal is to engineer the most miniscule parts of life, like proteins, to study or alter their function... -
Sequencing Options for CRISPR Genotyping
TypeBlog PostPublishedOct. 4, 2016, 2:30 p.m....address these concerns as CRISPR use grows across the life sciences and to establish standardized validation... -
Which Fluorescence Microscopy Technique is Best for Me?
TypeBlog PostPublishedOct. 10, 2017, 1:57 p.m....or a biologist imaging the smallest structures of life; the key to a great image is contrast. The human... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog PostPublishedAug. 15, 2017, 1:24 p.m....proteins, as it enables tracking throughout the lifetime of the cell. The past 15 years have seen a...