We narrowed to 683 results for: Mos

...While Streptococcus pyogenes Cas9 (SpyCas9) is the most used and well studied CRISPR system, N. meningitidis...work? Anti-CRISPRs proteins are highly diverse but most block CRISPR activity one of three ways: Inhibit...

... because I truly enjoy the field I am in, the atmosphere, and the people.” My absolute favorite response...Operations Specialist. She said, “I’ve found the most purpose at Addgene being myself. I like being with...

... the targeting scope of adenine base editors The most commonly used S. pyogenes Cas9 requires the target...ABE7.10 with ABE8.20-m reducing protein expression the most in primary human T cells. While editing single genes...

...prone to error — both human and biological. Even the most high-fidelity polymerases can make mistakes and ...and highly repetitive sequences and/or features. Most of these regions are identified during the QC process...

...Primer binding sites with 40–60% G/C content are most likely to be successful, although sequences with...which helps users select pegRNA designs that are most likely to drive efficient editing. This is not the...

...range of clinical manifestations. PMM2-CDG is the most frequent CDG type characterized by deficient phosphomannomutase...mortality. Since glycans play an essential role in almost every aspect of immunology, participating in events...

...in young macaques and vascular bias in mature marmosets. CAP-Mac also robustly expressed fluorescent reporters...(amino acid sequence DYKDDDDK) may be one of the most versatile. Whether you plan to purify or image a...

...depending on what cell type it is expressed in. The most common target for chemogenetic manipulation are ...have been derived from muscarinic receptors. The most popular agonist for muscarinic receptor derived ...

.... As the depositing scientist, you will have the most involvement with Step 1, and, while data entry can...every so often and this ensures you are using our most up-to-date version! We hope the deposit spreadsheet...

...astrocytes, and AAV4 targets mostly astrocytes. Lentiviral vectors are pseudotyped in most cases with VSV-G that...

...similar homology to the site of interest or create mosaic animals where not all cells in the blastocyst were...small molecule that regulates Cre expression. The most common drug used to induce the expression of Cre...

...well as schools, community events, churches or mosques. On occasion, we also get interviewed for print...fields of research, what often concerns them the most about research is its implications for their lives...

...some engineered terminators come close (>95%). For most purposes, however, any common terminator will suffice...Addgene (1). Prokaryotic polyadenylation Although mostly thought of as a eukaryotic-specific process, prokaryotes...

...the target cell’s genome. Physical titer The two most common assays for physical titer are direct p24 ...choice can also impact transgene expression. The most accurate method of lentiviral titration uses qPCR...

...you can gain experience working with other people mostly online and on monthly phone calls. This has its...in the format requested which in the long run was most beneficial for the organization as a whole. I came...

...DNA polymerase from the thermophilic bacterium, Thermos aquaticus (Taq). Taq polymerase is thus a heat ...presence, size, and relative quantity. End point PCR is most commonly used in molecular cloning, sequencing, ...

...that forms chromosomes. These long range DNA interactions are often detected with chromosome conformation...

...degrons, you have options! Below we will review the 4 most common types of degrons and some of the nuances ...degradation. HaloTag is also the degron tag with the most alternative functions — there are antibodies to ...

...suffers from several limitations. Perhaps most importantly, most Cas9 orthologs are less well characterized...

...One of the most powerful strategies to investigate a gene's function is to inactivate, or "knockout",...of the targeted gene will be removed from the chromosome and and replaced by the resistance gene.The gene...