We narrowed to 860 results for: PLE;

...arranged in a double helix. People are wrong. In reality, cellular DNA is a complex three-dimensional globule...Technique What It Locates ChIP-seq Proteins (for our example later, histones) ChiRP-seq RNA Hi-C DNA (genome-to-genome...appendix DNA is a 3D fractal). Enhancer biology is a complex and dynamic field. We’re going to focus on a tried... beyondthedish (left) and biobabel (right). People commonly think of DNA as a linear polymer of A,...Bedtools getFasta. These regions and their reverse complements were parsed for spCas9 PAM sites (NGG) and then...

...expertise on the processes needed to successfully implement, manage and close-out a grant. Grant professionals...reports are submitted and project evaluations are completed. To enter and advance the profession, you can ...  Skills such as research, collaboration across multiple disciplines, writing, project management, and ...application writing and development, project implementation, program evaluation and grant management.  ... time and resources. 3. Get a mentor, find your people Find someone who is working or has worked as a ...knowledge of the 8 core competencies through a multiple choice exam.  The bottom line on being a grants...

...to be managing. Being reminded of the seemingly simple actions we can take to help ourselves can be an...regular schedule at work. Choose a lab/job where people have regular schedules. Work 8 to 5 or 7 to 4 or...create “fake” family by forming a social circle of people in the same situation. Go to church, join a club... in the family to have to miss something – for example, say no to some of those birthday parties. Tell...print, but I actually let my daughter skip school a couple of times. Be sure to add time with your family,...time. For me, that’s been volunteer work at our temple, my kids’ school, and with professional organizations...

...Its RNA-guided nature makes CRISPRa much simpler to implement in genome-wide screens over cDNA libraries... Rochester Medical Center. During development, complex genetic programs specify and assemble diverse arrays...individual genes can be relatively laborious because multiple plasmids/viruses are needed to deliver all the... then packaged into AAV for in vivo delivery. Multiplex gene targeting is also possible and is only constrained...vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR–dCas9-activator...

...mechanisms behind the cytotoxicity can vary. For example, the popular VSV-G envelope protein causes cells...are differentially inactivated by nonspecific complement mechanisms in serum. Sometimes this is due to...cells that the viral vectors were produced in. For example, production of vesicular stomatitis virus, HIV-...sugars end up in the envelope and are targets for complement-based killing by anti-alphaGal antibodies. The...good at infecting most cells, but not all. For example, LDLR expression in hematopoietic stem cells, T... as described above and is inactivated by the complement system in serum (DePolo et al., 2000). Other ...

...al., guides were considered toxic if they were depleted from the screen. This toxicity was used a proxy... the growth screen, safe-targeting guides were depleted at greater rates than their non-targeting counterparts...Additionally, the distribution of enrichment and depletion scores for safe-targeting guides more closely ...noise, of dsDNA breaks caused by CRISPR. See supplemental figure 6 for more details. Safe-targeting guides...However, there was no significant difference in depletion of ricin sensitizer genes identified with short...Resources Watch Dr. Michael Bassik’s lecture on “Multiplexing with CRISPR Screens”    ...

...applying to, are doing - for example, your benefits may be changing. Please also feel free to contact us... salary was set at $47,476 and the date for implementation was set for Dec 1 2016. In order to be exempted...submitted letters to the Department of Labor (for example here and here) pushing for exemptions for postdocs...be on higher salaries (which have been made by multiple organizations across academia). Increased postdoc...wide consensus of academic stakeholders. There was ample opportunity to accept these recommendations, and...

...combinations. For example, with standard fluorescent microscope filter sets, users can label multiple proteins ...often have absolute position requirements. For example, a KDEL ER retrieval sequence only functions at... even disrupt fusion protein localization. For example, the formation of inappropriate inter-chain disulfide...disulfide bonds between FPs blocks Golgi complex-localized fusions from properly exiting the ER and fluorescing... dark fluorescent proteins in your experiments, please consider the following: How can one ensure that...

...revolutionised the way we apprehend molecular complexes and is still a very useful tool.    Like a guitar...technique, but it is very important to follow some simple rules to avoid common pitfalls and to use it at... These issues can be overcome by choosing a complementary pair of fluorophores. To maximize the FRET signal...especially when you investigate unknown molecular complexes. But it is a parameter that you have to keep in...most efficient approach for your own system. The simplest and the most popular one is the sensitised emission...photobleaching method The acceptor photobleaching method is simple but limited to a single measurement. This method...

...foothills, I began to have conversations with many people who eagerly follow progress in synthetic biology...channels, books, podcasts, and communities to help people get started in synthetic biology. While not everything...NCI, NIH | License   I’m updating it regularly as people send me suggestions. The most useful parts of the...biology can be difficult to find. I hope that many people will find this guide useful as they start their...have additional resources that might be helpful, please reach out to me by email.  About the author Niko...

...across multiple plasmids. For simplicity, we’ll use the second-generation system as an example.  The ...Lentiviral vectors have been a staple in molecular biology for over three decades. Widely used across...double-stranded DNA transgene is incorporated into a large complex of both viral and cellular proteins and trafficked...the delivered payload is gradually reduced. For example, after using CRISPR/Cas9 to edit a gene, the expression...performing quantitative PCR titration of vector DNA in samples harvested 24 hours post-transduction (Wanisch and...

...hotspot for dystrophin, exons 45-55, there are multiple common deletions that maintain the protein’s reading...regularly. Dystrophin and genome editing Since complex oligonucleotide treatment comes with many challenges...skipping? For such a therapy to be successful, multiple requirements must be met. First, CRISPR would ...dystrophy. gRNAs can be designed to flank a single or multiple exons that, when deleted through NHEJ, will restore...therapy, it’s important to realize that DMD is a simpler case than other genetic diseases we’d like to treat...Thakore PI, Majoros WH, Reddy TE, Gersbach CA. Multiplex CRISPR/Cas9-based genome editing for correction...

...undergraduate experiences, they were provided with ample resources and a positive perspective on the transition...career training approaches in a way that can be implemented widely in the community.  Talk alone is not going...resulting in concrete actions and outcomes. For example, pd|hub assembled a coalition of higher education...Sciences (NIGMS) will support the development and implementation of collections of evidence-based practices.... disseminated by “train the trainer” programs.  Please join us for the pd|hub Workshop Report Public Release...

...below the citation. An example of these "Associated Plasmids" is shown in the purple box below, where the...Now you can learn how a plasmid has been used by multiple labs and see what experimental systems it has ...right column of the plasmid page. Check out the purple arrow in the screenshot below to see what I mean...the dropdown to increase the length of the list (purple oval in the screenshot below). You can also use...

...Using AAV vectors in complex biological systems can be tricky at best, and downright infuriating at worst... that several steps in testing are effected by multiple AAV variables.    Serotypes Serotype refers...what works great in one system or cell type can completely fail in a different system (Issa et al., 2023...postsynaptic transmission (Oh et al., 2020). For example, AAV1 and AAV9 tend to spread farther from the ...Off-target expression can still occur (Fig. 3) - for example, some neurons can transiently express TH - so you..., 2010).  Second, high efficiency transduction coupled with a strong promoter, may result in overexpression...

...Viruses (5). Please refer to the paper for more details on the assay, including examples of RBPs successfully...faces as it approaches a host cell surface are complex polysaccharide structures, many of which are easily... genome amplification (by a mechanism known as multiple displacement amplification) even in the presence... of 10-20 colonies using primers bordering the multiple cloning site of the vector. In order to reduce...others in liquid culture, dilute and plate the complete library of transformants for single colonies (...

...Mentorship Program? In an era where millions of people hold a PhD, achieving our career goals can be a...with and be inspired by the mentor’s successful example. For evidence of this, you needn’t look any further...cancer. She has guided me in my next steps. For example, how to get a fellowship to travel abroad, how ... a post-program self-assessment, participants completed follow-up self assessments and program evaluations...result directly in positive impacts on the lives of people and society. Future generations of IMP Motivating... MD, PhD, is a Board Certified neurologist and multiple sclerosis specialist, currently President and ...

...finishing your PhD or perhaps you have almost completed a postdoctoral position… or two. You have learned...less experienced grads Manage a project involving multiple scientists, create and stick to timelines Be a...collaborations to advance science faster. Help other people when you can. The colleagues you help will be future...another field maybe, and publish Join a lab that has multiple collaborations, even with Industry labs Work on...Truth About Soft Skills—Workplace Lessons Smart People Wish They’d Learned Sooner by Peggy Klaus Looking...

...King soundtrack and writing this post, it is my pleasure to announce that we once again reached new heights...and all those who have helped edit over the past couple of months. Read on to discover what new post contributed...P1 phage. 2) The post describes an ingenious but simple technique to screen for proteins that could be .... FOP), that, while affecting a small number of people, deserves our attention and has huge potential ...Symposium Tips for Writing a Good Cover Letter Multiple Plasmids at a Low Price: Kits from Addgene Savvy...

...hope of accomplishing it all. How do we encourage people to take a break from their everyday tasks and make... everyone at Addgene, and send random sets of 4 people out to lunch.   I think these random lunches would...these lunches would provide a friendly setting for people in different labs to chat informally about their...about helping scientists share between labs, and people work hard at Addgene to make plasmid sharing as...to take a break from work to share time with the people around us. It makes the quality of our work better...