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Showing: 381 - 400 of 606 results

  1. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...) to remove mutated exons from the dystrophin transcript. Unfortunately, the oligonucleotides only modestly...in one experiment, they found the exon-skipped transcript constituted 59% of total dystrophin mRNA, similar...

  2. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...”, “colony”, etc. This will help save time by screening out irrelevant candidates, and, more importantly...importantly, it’ll help you define salary costs. 4. Ascribe a cost structure associated to these tools, skills...

  3. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...2025).In this latest work, the team designed and screened a large library of iGluSnFR3 mutants, identifying... insertion, a combined endonuclease-reverse transcriptase uses homology arms flanking an RNA payload to...

  4. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ... but many of these tips apply to all tissues. Subscribe to Viral Vector Blog Posts from Addgene 1. Location...and can immediately undergo replication and transcription. However, with scAAV the effective genome size...

  5. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ... appealing to researchers.  TALENs In 2010, transcription activator-like effector nucleases (TALENs) were... which is a fusion between Cas9 and reverse transcriptase. Using an elongated template + guide RNA called...

  7. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    .... Bell, Charles C., et al. "A high-throughput screening strategy for detecting CRISPR-Cas9 induced mutations...Learn to Use Cas9 Activators Perform Genome-Wide Screens with CRISPR Generate Mouse Models with CRISPR/Cas9...

  8. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...performing several rounds of random mutagenesis screening for enhanced RFP variants. PA-mCherry (E26V / ...photoswitching. In 2008, scientists used mutagenesis screens to find a version of Dronpa that doesn't become...

  9. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ... tested and will help healthcare professional prescribe and identify potential beneficiaries. Collaborative...Collection Pages Plasmid Grant to Aid Cervical Cancer Screening in Ghana Modulate Cancer Signalling Resources...

  10. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...interference, as it can target and degrade select RNA transcripts, or be engineered to conduct direct RNA editing...-Aslan, A., & Marraffini, L. A. (2015). Co-transcriptional DNA and RNA Cleavage during Type III CRISPR-Cas...

  11. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...the CGBE architecture. They conducted a CRISPRi screen to identify endogenous genes that impact CGBE efficiency...•G-to-G•C base editors developed using CRISPRi screens, target-library analysis, and machine learning....

  12. New Antibody Animation!

    Type
    Blog Post
    Published
    Feb. 22, 2024, 3:13 p.m.
    ...different antibody isotypes found in mammals.      Subscribe to Addgene's YouTube channel for more helpful ...

  13. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    Published
    June 2, 2017, 2:30 p.m.
    ... you're designing your next virus experiment. Subscribe to Viral Vector Blog Posts from Addgene Need ...

  14. Introducing...Addgene Quick Tips!

    Type
    Blog Post
    Published
    Sept. 19, 2024, 3 p.m.
    ...every quick tip video on our TikTok! Be sure to subscribe to our YouTube channel for quick tips, protocols...

  15. A Case Study of Addgene Bioinformatics

    Type
    Blog Post
    Published
    May 25, 2023, 1:15 p.m.
    ...of our Data Team, “Our patchwork collection of scripts evolved into a single, organized pipeline and accelerated...

  17. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    Published
    Dec. 28, 2021, 2:15 p.m.
    ...  Can't get enough of CRISPR? Neither can we! Subscribe below to get updates on all our CRISPR content...

  20. The Twelve Days of CRISPR

    Type
    Blog Post
    Published
    Dec. 22, 2020, 2:15 p.m.
    ...been enjoying our CRISPR blog series. Be sure to subscribe to the CRISPR blog posts so you don’t miss a thing...
Showing: 381 - 400 of 606 results