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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 848 results for: STI

Showing: 381 - 400 of 848 results

  1. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...Pederson’s lab used CRISPR to label multiple loci in distinct colors. To create a colorful Cas9 toolbox, Ma ... different fluorescent protein to create three distinct colors. The specificity of these orthologs is ...yellow, cyan, or magenta light. A gRNA with all 3 distinct hairpin sequences produces white light. Created...this rainbow of colors, Ma et al. performed sophisticated tracking of multiple chromosomal loci in live...

  2. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...Shaner et al, 2008). This brings us to the key question: what is the best way to compare the photostability...producing the fluorescent protein of interest under realistic conditions, i.e. with low excitation power. By...power experiments. Measuring photostability at realistic powers will provide better insight into the photostability...photostability in living cells provides a much more realistic view of photostability. Selecting a photostable...

  3. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ..., a postdoctoral fellow at Dana-Farber Cancer Institute. Targeted protein degradation In the Bradner and.... Remarkably, small molecule degraders provide distinct advantages over pharmacological inhibitors including...address your requests for dTAG molecules and any questions to Behnam Nabet ([email protected] fellow at the Dana-Farber Cancer Institute. He is a chemical biologist interested in targeted...

  4. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ...chemically modified to resist degradation while still effectively serving their function of guiding Cas9...at the same site, is 2’-fluoro (2’-F), which substitutes a fluorine for the hydroxyl group at the 2’ position...advantages, bridged nucleic acids with N-methyl substitutions are slightly more efficient and less toxic to...molecule) you will not have that option, but you can still choose what dye to use  What to do once you’ve chosen...

  5. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...one is not available, don’t lose hope! It could still work. Take a close look at the antigen or antigenic...you will need to make sure that you can easily distinguish each different type of antibody and therefore... through the above steps, you may find that you still have a number of options. If this is the case, you...your assays up and running.  Ed.'s note: More questions on antibody validation? Check out Antibodies 101...

  6. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    Published
    Nov. 24, 2015, 3:30 p.m.
    ...repurpose popular GFP reporter lines for more sophisticated experimental manipulations, saving the time ...experienced: wanting to ask and answer experimental questions, but not having the tools needed to do so. Cepko... using GFP as a scaffold suddenly seemed very realistic. Envisioning GFP as a substrate promoting dimerization...Transcriptional Devices Dependent On GFP). In testing the specificity of their system, Tang et al. found...

  7. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    ...many important questions, areas for informed discussion, debate, and data collection still remain: What...workforce structure? Helping to answer some of these questions, and posing some of their own are the following...grassroots organisations that represent postdocs and institutes that employ them, the diversity of trainee backgrounds...

  8. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ...funding from a variety of government and academic institutions. New graduate students may assume that their...the mission of the application and the funding institution. Moreover, avoidance of ‘to be’ verbs creates... with the description of the RFA and funding institution. Emphasizing how your solution fits into the ...research project grant application" by the NIH Still want more information on the application process...

  9. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...plasmids can lead to far reaching consequences, from wasting time and money on optimizing experiments, to false...all incoming plasmids. If you are interested in requesting one of these plasmids, you can request that it...reference is given, de novo assembly in Geneious can still provide additional insights to resolve the assembly...team leaves a note on the plasmid page to make requesting scientists aware of the identified discrepancy...

  10. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 1, 2018, 1:57 p.m.
    ...and overlapping complexes that have distinct functions. Many existing methods for identifying PPIs, such...additional interacting factors. Problems with Existing Methods for Studying Protein-Protein Interactions... biotinylated. This makes it’s impossible to distinguish proteins that directly interact with the bait...

  11. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...glycerol stock. Choose from: ampicillin, apramycin, blasticidin, bleocin (zeocin), chloramphenicol, erythromicin...box for the “Purpose” field. If you ever have a question about what should go into a cell, hover over the...Distribute pending QC. The name of the Principal Investigator and Organization where your plasmids were first...in March 2017 and updated by Angela Holmes and Christina Mork in February 2024.    Additional Resources...

  12. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ...transduced cells and loss of transgene expression (Interestingly, the strong immunogenicity of AdVs is what makes...virion is a non-enveloped particle of ~100 nm, consisting of a capsid surrounding an inner core which contains...use of this system. New and improved systems are still being developed. A detailed protocol for production...mL of sample. The purity of the sample can be estimated by looking at the ration of DNA (260nm) versus...

  13. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...and application should be considered The first question you may have is whether a monoclonal or polyclonal... 1998). Consequently, if you find yourself in a sticky species-on-species situation, and other antibody...that users choose antibodies that are easily distinguishable from each other. In a direct approach, that... species or isotype that can be probed with a distinctly conjugated secondary antibody. Consider too that...

  14. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...pattern of the antibody binding “distinct imaged pattern.” The distinct imaged pattern should align with...comparison on the antibody signal’s location. If a distinct imaged pattern does NOT align with the atlases...through their own analysis of the background noise, distinct imaged pattern, and exposure times. To test the...

  15. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...changeover had no impact on our ability to provide requesting scientists high quality data! Addgene's new ... example, the CAG promoter, a hybrid promoter consisting of a CMV enhancer, chicken beta actin promoter... that is useful, but not 100% complete, we will still make this data available as a partial sequence with...develop a PCR strategy suitable for the library in question. We will order primers that can amplify the insert...

  16. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ... to modify chromatin. A fluorescent protein can still be included for visualization purposes, but the ...flexibility of the single-pot system will allow investigators to rapidly create multiple synthetic chromatin-modifying...CRISPR plasmids for Borrelia burgdorferi from Christine Jacobs-Wagner’s lab produce different levels of...pooled library from Keith Joung’s and Benjamin Kleinstiver’s labs are now available. These libraries encode...

  17. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...impressive, full-color images of proteins. But they are still flat. To truly appreciate the 3D structure of these...models function as thinking tools – eliciting questions like “what does this model representt?" ... "how...creating physical models of proteins that are being investigated in their labs. Soon after we began working with...of the night dreaming about the conformational gymnastics that this protein must undergo as it interrogates...

  18. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    Published
    July 13, 2017, 2:30 p.m.
    ...important to isolate females from males while they are still virgins. Females begin mating ~6 hours after hatching...the Classical Genetics Simulator website. One interesting thing to know is that males do not recombine ... of Fly Pushing by Ralph J. Greenspan. It’s a fantastic book for newbies on how to work with flies. ... convince him to use Twitter @jonchowphd but he still reads “#” as a “pound symbol”. Additional Resources...

  19. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...kinds of CRISPR tools people were depositing and requesting each year (Figure 2). We did a similar analysis...2012 to 2023; data are smoothed using locally estimated scatterplot smoothing. (C) Number of requests ...continue to develop and share new tools, while our requesting labs continue to request and use new tools. Because... each country. Countries shown are the top 10 requesting countries. FR=France; KR=South Korea; CH=Switzerland...

  20. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...Restriction digest screening for ITR deletion is still necessary. To save time and money, I like to miniprep...Production protocol, I take a lazier approach for harvesting my AAV. I skip the PEG precipitation of the HEK293...PEG solution. PEG takes several hours on a heated stir plate to dissolve and requires monitoring so that...autoclaving, which also takes time. Rather than investing all of this effort in preparing reagents, I choose...
Showing: 381 - 400 of 848 results