We narrowed to 859 results for: STI

...chemically modified to resist degradation while still effectively serving their function of guiding Cas9...at the same site, is 2’-fluoro (2’-F), which substitutes a fluorine for the hydroxyl group at the 2’ position...advantages, bridged nucleic acids with N-methyl substitutions are slightly more efficient and less toxic to...molecule) you will not have that option, but you can still choose what dye to use  What to do once you’ve chosen...

...experiment.  For example, I used to constantly underestimate time needed to prep for an experiment (labeling...protocol, from a full page to a few key reminders on a sticky note   Visual Planning  Plate maps, pre-labeling...you need to be successful! Slowing Down At the suggestion of a postdoc, to whom I am forever grateful, ... of things, including (and maybe especially) suggestions you are initially resistant to. Just like any...

...answer to any of these questions is “yes,” then I would suggest either testing the antibody efficacy through...incident to your records, and consider the following questions as you move forward:  Was a light-sensitive conjugate...for your ELISA and Western, I’d highly recommend sticking with what works.  To balance this out, however...

...biology – they can deliver materials to specific destinations, haul a variety of cargo, and even handle a ...cassettes typically contain a promoter (inducible or constitutive), your gene of interest, and often a selectable...your construct in target cells transiently or constitutively depending on the virus used. All of these ...response 2) very high immunogenicity 3) and long-lasting immune response even after a single dose. ...

...a talk about the different parts of the brain stimulated by stories. In retrospect, I should have expected...neuroscience and you don’t have a background in statistics and programming, you’d be wise to start working...surprised at how easily I could jump in and ask questions about the various posters I visited. If you’re...neuroscientist looking to get into the field, this is a fantastic time to do it! Additional Resources on the Addgene...

...focus of the application was changed, but Sample6 still leverages the core engineering and scientific concepts...communication styles in academia and industry vary drastically, he reassures that one can learn it by reading...into the local academic community. However, Mike still could not remove himself from the idea of starting... many universities throw out old equipment that still works. “We use functional spectrometers from the...

...Mentoring series will cover 2 of the 7 mentoring questions I set out to answer. First, I will describe some...diligent about replying and thinking about good questions and answers. Professional networking groups will...and move on. If they say yes, be prepared with questions and topics to discuss. The mentee is responsible... needs to be open to change and constructive suggestions and feedback. The best thing to do to make your...

...Shaner et al, 2008). This brings us to the key question: what is the best way to compare the photostability...producing the fluorescent protein of interest under realistic conditions, i.e. with low excitation power. By...power experiments. Measuring photostability at realistic powers will provide better insight into the photostability...photostability in living cells provides a much more realistic view of photostability. Selecting a photostable...

...Pederson’s lab used CRISPR to label multiple loci in distinct colors. To create a colorful Cas9 toolbox, Ma ... different fluorescent protein to create three distinct colors. The specificity of these orthologs is ...yellow, cyan, or magenta light. A gRNA with all 3 distinct hairpin sequences produces white light. Created...this rainbow of colors, Ma et al. performed sophisticated tracking of multiple chromosomal loci in live...

...one is not available, don’t lose hope! It could still work. Take a close look at the antigen or antigenic...you will need to make sure that you can easily distinguish each different type of antibody and therefore... through the above steps, you may find that you still have a number of options. If this is the case, you...your assays up and running.  Ed.'s note: More questions on antibody validation? Check out Antibodies 101...

... post was written by Professor Mark Howarth, investigator at the University of Cambridge. His research...the features that would define a good tool for sticking together biological components. Nearly all of ...of us played with LEGO as a child (some of us still do), so we are used to the idea of general interfaces...Biology reactions can add new functionality to existing genes, while being accessible simply by dialing...

..., a postdoctoral fellow at Dana-Farber Cancer Institute. Targeted protein degradation In the Bradner and.... Remarkably, small molecule degraders provide distinct advantages over pharmacological inhibitors including...address your requests for dTAG molecules and any questions to Behnam Nabet ([email protected] fellow at the Dana-Farber Cancer Institute. He is a chemical biologist interested in targeted...

...repurpose popular GFP reporter lines for more sophisticated experimental manipulations, saving the time ...experienced: wanting to ask and answer experimental questions, but not having the tools needed to do so. Cepko... using GFP as a scaffold suddenly seemed very realistic. Envisioning GFP as a substrate promoting dimerization...Transcriptional Devices Dependent On GFP). In testing the specificity of their system, Tang et al. found...

...many important questions, areas for informed discussion, debate, and data collection still remain: What...workforce structure? Helping to answer some of these questions, and posing some of their own are the following...grassroots organisations that represent postdocs and institutes that employ them, the diversity of trainee backgrounds...

...funding from a variety of government and academic institutions. New graduate students may assume that their...the mission of the application and the funding institution. Moreover, avoidance of ‘to be’ verbs creates... with the description of the RFA and funding institution. Emphasizing how your solution fits into the ...research project grant application" by the NIH Still want more information on the application process...

...glycerol stock. Choose from: ampicillin, apramycin, blasticidin, bleocin (zeocin), chloramphenicol, erythromicin...box for the “Purpose” field. If you ever have a question about what should go into a cell, hover over the...Distribute pending QC. The name of the Principal Investigator and Organization where your plasmids were first...in March 2017 and updated by Angela Holmes and Christina Mork in February 2024.    Additional Resources...

...plasmids can lead to far reaching consequences, from wasting time and money on optimizing experiments, to false...all incoming plasmids. If you are interested in requesting one of these plasmids, you can request that it...reference is given, de novo assembly in Geneious can still provide additional insights to resolve the assembly...team leaves a note on the plasmid page to make requesting scientists aware of the identified discrepancy...

...changeover had no impact on our ability to provide requesting scientists high quality data! Addgene's new ... example, the CAG promoter, a hybrid promoter consisting of a CMV enhancer, chicken beta actin promoter... that is useful, but not 100% complete, we will still make this data available as a partial sequence with...develop a PCR strategy suitable for the library in question. We will order primers that can amplify the insert...

... to modify chromatin. A fluorescent protein can still be included for visualization purposes, but the ...flexibility of the single-pot system will allow investigators to rapidly create multiple synthetic chromatin-modifying...CRISPR plasmids for Borrelia burgdorferi from Christine Jacobs-Wagner’s lab produce different levels of...pooled library from Keith Joung’s and Benjamin Kleinstiver’s labs are now available. These libraries encode...

...pattern of the antibody binding “distinct imaged pattern.” The distinct imaged pattern should align with...comparison on the antibody signal’s location. If a distinct imaged pattern does NOT align with the atlases...through their own analysis of the background noise, distinct imaged pattern, and exposure times. To test the...