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We narrowed to 608 results for: Sis;

Showing: 381 - 400 of 608 results

  1. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...physical structure, separate chromosomes during mitosis, support transport of organelles and vesicles, ...the samurai sword called a katana. Opto-katanin consists of two separate blue light-sensitive systems, ...

  2. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ..., Y., & Peng, C. (2018). Overview of MicroRNA biogenesis, mechanisms of actions, and circulation. Frontiers...Ørom, U. a. V. (2023). Recent progress in miRNA biogenesis and decay. RNA Biology, 21(1), 36–43. https:/...

  3. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ... et al. 2022). Lactate is a key metabolite in glycolysis, so this sensor will be a valuable tool to study...lifetime imaging. B) LiLac responses are highly resistant to pH changes. Image adapted from Koveal et al...

  4. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ... steps: 1. Define your timeline Start with your thesis in mind. Imagine the kind of data your dream discovery...consultant and coach in Boston, MA. He specializes in assisting research scientists create productive and manageable...

  5. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...advantage of CRISPR epigenetic tools is their persistence and inheritance. CRISPR activators and repressors... culture. However, DNMT3A-induced methylation persisted throughout a 100 day experimental period, as this...

  6. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...optogenetic tools derived from direct comparative analysis of microbial opsins. Nature Methods, 9(2), 159...

  7. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...Aschauer, D. F., Kreuz, S., & Rumpel, S. (2013). Analysis of Transduction Efficiency, Tropism and Axonal...Hosokawa, Y., & Konishi, H. (2014). A comparative analysis of constitutive promoters located in adeno-associated...

  8. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...generate more signal from low abundance targets. Analysis/Presentation Once you’ve captured your images,...imaging approach for deep phenotyping and spatial analysis of cells in complex tissues. Proceedings of the...

  9. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...be used for numerous applications, including transgenesis, gene-trap screens, and gene editing. How does... Verma, “Functional Gene Correction for Cystic Fibrosis in Lung Epithelial Cells Generated from Patient...

  12. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...protein tags. The basics These tags most often consist of an enzyme that has been mutated to block its...Labeling Technology for Cell Imaging and Protein Analysis. ACS Chemical Biology, 3(6), 373–382. https://...

  15. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...the complexity of the screen. For example, the analysis becomes easier, the number of cells needed is ...add value if you are addressing a well-defined, hypothesis driven research question. Examples of custom ...

  16. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...distinguish different target proteins in your flow analysis, you'll need to use fluorophores that attach to...you can separately detect these markers in your analysis. Lastly, there are fixable dye stains that react...

  17. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...contributed by guest blogger James D. Fessenden, an Assistant Professor at Brigham and Women’s Hospital. Biochemists...Fessenden! James D. Fessenden is currently an Assistant Professor in the Anesthesia Research department...

  19. We’re Going Viral This Week!

    Type
    Blog Post
    Published
    Oct. 14, 2019, 1 p.m.
    ...strategies to improve viral vector production and data analysis. Our first paper came out last year on sucrose...

  20. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    Published
    April 1, 2022, 1:15 p.m.
    ...itself. Many of us have experienced the minor yet consistent annoyance of walking into a fly lab and finding...
Showing: 381 - 400 of 608 results