We narrowed to 543 results for: TIL

1. Synthetic Photobiology: Optogenetics for E. coli

   Type

   Blog Post

   Published

   Sept. 8, 2015, 2:30 p.m.

   ...condensed the system to two streamlined plasmids. The utility of these TCSs was limited by a few factors. These...

2. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

   Type

   Blog Post

   Published

   Aug. 28, 2014, 3:34 p.m.

   ...samples of similar size. Run the gel at 80-150V until you have good separation between your bands. Stopping...

3. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

   Type

   Blog Post

   Published

   May 3, 2018, 1:48 p.m.

   ...similar cleavage products even when guides were tiled across a target RNA, indicating that this enzyme...

4. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

   Type

   Blog Post

   Published

   June 6, 2019, 12:41 p.m.

   ... also created. The depositors demonstrated the utility of light-mediated suppression of synaptic transmission...

5. Targeted Mutagenesis with EvolvR

   Type

   Blog Post

   Published

   Feb. 21, 2019, 1:15 p.m.

   ...loci in bacteria. Its modular nature provides versatility in defining the mutational rate and editing window...

6. Luminescent Imaging with Nano-lanterns

   Type

   Blog Post

   Published

   May 25, 2017, 2:30 p.m.

   ...based on Nano-lantern, showing the technique’s versatility and adaptability to many different contexts. ...

7. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

   Type

   Blog Post

   Published

   May 11, 2017, 2:30 p.m.

   ... many tricks up their sleeves and most patients still don’t benefit from this approach. One central bottleneck...

8. Screening for Successful Genome Editing with Digital PCR

   Type

   Blog Post

   Published

   March 30, 2017, 2:30 p.m.

   ...although digital PCR is a great screening tool, it still requires target DNA sequencing to confirm the exact...

9. A Quick Guide to a Career in Software Product Management

   Type

   Blog Post

   Published

   Nov. 24, 2020, 2:15 p.m.

   ...they may need to help improve their work. Then, I distill and translate that valuable information back to...

10. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type

    Blog Post

    Published

    Aug. 20, 2024, 1:15 p.m.

    ...that shifts to the left. When using MFI, you can still continue gating populations of interest with normal...

11. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type

    Blog Post

    Published

    Aug. 6, 2024, 1:15 p.m.

    ...using helper-free packaging cell lines This method utilizes a packaging cell line that stably expresses Gag-Pol...

12. Hot Plasmids - May 2022

    Type

    Blog Post

    Published

    May 26, 2022, 1:15 p.m.

    ...culture and whole organisms (see our 2019 overview). Until recently, the approach has been hampered by the ...

13. AAV Q&A with Tim Miles

    Type

    Blog Post

    Published

    May 17, 2022, 1:15 p.m.

    ...questions via text! (well, maybe not all of them. We still haven’t gotten to the bottom of why hot dogs are...

14. Clear and Accessible Writing

    Type

    Blog Post

    Published

    Feb. 15, 2024, 2:15 p.m.

    ...supernatant migrates through increasingly dense layers until the macromolecule density is equal to the buoyant...

15. CasPEDIA: A Functional Classification of Cas Enzymes

    Type

    Blog Post

    Published

    Jan. 25, 2024, 2:15 p.m.

    ... However, many situations could arise where you still need more information. If you are performing a viral...

16. Viral Vectors 101: Inverted Terminal Repeats

    Type

    Blog Post

    Published

    Oct. 19, 2023, 1:15 p.m.

    ... efficiency. Our practical knowledge of ITRs is still limited, but this area of research is important ...

17. Viral Vectors 101: Viral Vector Elements

    Type

    Blog Post

    Published

    July 25, 2023, 1 p.m.

    ...into the host genome (more on this below), it’s still used to deliver gene expression constructs. Sandwiched...

18. Viral Vectors 101: Biosensors

    Type

    Blog Post

    Published

    July 6, 2023, 1 p.m.

    ...you covered with viral resources and safety tips. Still worried? We make virus with some of our most popular...

19. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type

    Blog Post

    Published

    Jan. 21, 2025, 2:15 p.m.

    ... "do it yourself" can result in burnout and underutilized team potential. I often thought that performing...

20. Viral Vectors 101: Viral Applications

    Type

    Blog Post

    Published

    April 13, 2023, 1:15 p.m.

    ...Additional Resources on the Addgene Blog AAV: A Versatile Viral tool for Gene Expression in Mammals Listen...