We narrowed to 546 results for: TIL;

...repair that normally accompanies Cas9 cutting. We still don’t know the mechanism underlying dCas9 editing...

...condensed the system to two streamlined plasmids. The utility of these TCSs was limited by a few factors. These...

...samples of similar size. Run the gel at 80-150V until you have good separation between your bands. Stopping...

...based on Nano-lantern, showing the technique’s versatility and adaptability to many different contexts. ...

... many tricks up their sleeves and most patients still don’t benefit from this approach. One central bottleneck...

...although digital PCR is a great screening tool, it still requires target DNA sequencing to confirm the exact...

...similar cleavage products even when guides were tiled across a target RNA, indicating that this enzyme...

... inducible tetracycline ON (Tet-On) system, a versatile tool developed for use in prokaryotes and eukaryotes...

...they may need to help improve their work. Then, I distill and translate that valuable information back to...

... of a T7 RNA polymerase and cannot be expressed until the base editor corrects early stop codons within...

...culture and whole organisms (see our 2019 overview). Until recently, the approach has been hampered by the ...

...questions via text! (well, maybe not all of them. We still haven’t gotten to the bottom of why hot dogs are...

... also created. The depositors demonstrated the utility of light-mediated suppression of synaptic transmission...

...loci in bacteria. Its modular nature provides versatility in defining the mutational rate and editing window...

... Their long-lasting popularity illustrates the utility these tools have in many different areas of biology...

...using them to my professional advantage. It wasn’t until I wrote a blog post about a research paper that ...

...supernatant migrates through increasingly dense layers until the macromolecule density is equal to the buoyant...

... However, many situations could arise where you still need more information. If you are performing a viral...

... efficiency. Our practical knowledge of ITRs is still limited, but this area of research is important ...

...into the host genome (more on this below), it’s still used to deliver gene expression constructs. Sandwiched...