We narrowed to 546 results for: TIL;

1. R Bodies: Membrane-Rupturing Microscopic Tools

   Type

   Blog Post

   Published

   April 14, 2016, 2:30 p.m.

   ...extends to rip the bacteria apart; however, they still remain attached to the ciliate. It’s thought that...

2. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

   Type

   Blog Post

   Published

   March 24, 2016, 2:30 p.m.

   ...repair that normally accompanies Cas9 cutting. We still don’t know the mechanism underlying dCas9 editing...

3. Synthetic Photobiology: Optogenetics for E. coli

   Type

   Blog Post

   Published

   Sept. 8, 2015, 2:30 p.m.

   ...condensed the system to two streamlined plasmids. The utility of these TCSs was limited by a few factors. These...

4. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

   Type

   Blog Post

   Published

   Aug. 28, 2014, 3:34 p.m.

   ...samples of similar size. Run the gel at 80-150V until you have good separation between your bands. Stopping...

5. From Lab Bench to Leadership: Transitioning into Management as a PhD

   Type

   Blog Post

   Published

   Jan. 21, 2025, 2:15 p.m.

   ... "do it yourself" can result in burnout and underutilized team potential. I often thought that performing...

6. Flow Cytometry Readouts: Yes, No, and Everything in Between

   Type

   Blog Post

   Published

   Aug. 20, 2024, 1:15 p.m.

   ...that shifts to the left. When using MFI, you can still continue gating populations of interest with normal...

7. Viral Vectors 101: Gamma-Retroviral Packaging Systems

   Type

   Blog Post

   Published

   Aug. 6, 2024, 1:15 p.m.

   ...using helper-free packaging cell lines This method utilizes a packaging cell line that stably expresses Gag-Pol...

8. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

   Type

   Blog Post

   Published

   June 6, 2019, 12:41 p.m.

   ... also created. The depositors demonstrated the utility of light-mediated suppression of synaptic transmission...

9. Targeted Mutagenesis with EvolvR

   Type

   Blog Post

   Published

   Feb. 21, 2019, 1:15 p.m.

   ...loci in bacteria. Its modular nature provides versatility in defining the mutational rate and editing window...

10. 15 Years of Addgene: The Top 15 Plasmids

    Type

    Blog Post

    Published

    Jan. 8, 2019, 1:54 p.m.

    ... Their long-lasting popularity illustrates the utility these tools have in many different areas of biology...

11. Early Career Researcher Toolbox: Social Media for Scientists

    Type

    Blog Post

    Published

    Dec. 11, 2018, 12:46 p.m.

    ...using them to my professional advantage. It wasn’t until I wrote a blog post about a research paper that ...

12. Hot Plasmids - May 2022

    Type

    Blog Post

    Published

    May 26, 2022, 1:15 p.m.

    ...culture and whole organisms (see our 2019 overview). Until recently, the approach has been hampered by the ...

13. AAV Q&A with Tim Miles

    Type

    Blog Post

    Published

    May 17, 2022, 1:15 p.m.

    ...questions via text! (well, maybe not all of them. We still haven’t gotten to the bottom of why hot dogs are...

14. Clear and Accessible Writing

    Type

    Blog Post

    Published

    Feb. 15, 2024, 2:15 p.m.

    ...supernatant migrates through increasingly dense layers until the macromolecule density is equal to the buoyant...

15. CasPEDIA: A Functional Classification of Cas Enzymes

    Type

    Blog Post

    Published

    Jan. 25, 2024, 2:15 p.m.

    ... However, many situations could arise where you still need more information. If you are performing a viral...

16. Viral Vectors 101: Inverted Terminal Repeats

    Type

    Blog Post

    Published

    Oct. 19, 2023, 1:15 p.m.

    ... efficiency. Our practical knowledge of ITRs is still limited, but this area of research is important ...

17. Viral Vectors 101: Viral Vector Elements

    Type

    Blog Post

    Published

    July 25, 2023, 1 p.m.

    ...into the host genome (more on this below), it’s still used to deliver gene expression constructs. Sandwiched...

18. Viral Vectors 101: Biosensors

    Type

    Blog Post

    Published

    July 6, 2023, 1 p.m.

    ...you covered with viral resources and safety tips. Still worried? We make virus with some of our most popular...

19. Viral Vectors 101: Viral Applications

    Type

    Blog Post

    Published

    April 13, 2023, 1:15 p.m.

    ...Additional Resources on the Addgene Blog AAV: A Versatile Viral tool for Gene Expression in Mammals Listen...

20. A Quick Guide to a Career in Software Product Management

    Type

    Blog Post

    Published

    Nov. 24, 2020, 2:15 p.m.

    ...they may need to help improve their work. Then, I distill and translate that valuable information back to...