We narrowed to 546 results for: TIL;

...repair that normally accompanies Cas9 cutting. We still don’t know the mechanism underlying dCas9 editing...

...condensed the system to two streamlined plasmids. The utility of these TCSs was limited by a few factors. These...

...samples of similar size. Run the gel at 80-150V until you have good separation between your bands. Stopping...

...culture and whole organisms (see our 2019 overview). Until recently, the approach has been hampered by the ...

...questions via text! (well, maybe not all of them. We still haven’t gotten to the bottom of why hot dogs are...

...they may need to help improve their work. Then, I distill and translate that valuable information back to...

... of a T7 RNA polymerase and cannot be expressed until the base editor corrects early stop codons within...

... can be difficult to replicate. Unclear methods Until someone attempts to replicate the results of another...

...supernatant migrates through increasingly dense layers until the macromolecule density is equal to the buoyant...

... However, many situations could arise where you still need more information. If you are performing a viral...

... efficiency. Our practical knowledge of ITRs is still limited, but this area of research is important ...

...into the host genome (more on this below), it’s still used to deliver gene expression constructs. Sandwiched...

...you covered with viral resources and safety tips. Still worried? We make virus with some of our most popular...

...Additional Resources on the Addgene Blog AAV: A Versatile Viral tool for Gene Expression in Mammals Listen...

... "do it yourself" can result in burnout and underutilized team potential. I often thought that performing...

...that shifts to the left. When using MFI, you can still continue gating populations of interest with normal...

...similar cleavage products even when guides were tiled across a target RNA, indicating that this enzyme...

... inducible tetracycline ON (Tet-On) system, a versatile tool developed for use in prokaryotes and eukaryotes...

...interested in using AAVs because they provide a highly versatile means of delivering genes to many different cell...

...based on Nano-lantern, showing the technique’s versatility and adaptability to many different contexts. ...