We narrowed to 427 results for: Trac;
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TypeBlog PostPublishedAug. 20, 2020, 2:30 p.m....When choosing a Cas9, remember to check that your tracrRNA and crRNA (or synthetic gRNA) are derived from...
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Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog PostPublishedFeb. 18, 2020, 2:15 p.m....people within similar fields (ASM Microbe has eight tracks), something we didn’t have enough people to do ... -
An Inside Look into Addgene's Dev Team
TypeBlog PostPublishedJan. 23, 2020, 2:45 p.m....Addgenies, development includes everything from tracking plasmids from the moment a deposit kit is sent... -
Hot Plasmids: Fall 2024
TypeBlog PostPublishedNov. 21, 2024, 1:30 p.m....with Cas9 on an integrative plasmid containing tetracycline repressor TetR and resistance selection marker... -
Antibodies 101: Reading a Flow Plot
TypeBlog PostPublishedFeb. 6, 2024, 3:13 p.m....., Pons, J. A., & Muro, M. (2018). In vitro intracellular IFNγ, IL-17 and IL-10 producing T cells correlates... -
How to Write a Scientific Review Article
TypeBlog PostPublishedFeb. 16, 2017, 3:30 p.m....This is normal and it means you’re on the right track! Get feedback After you’re done editing, send it... -
Antibodies 101: Immunoprecipitation
TypeBlog PostPublishedDec. 7, 2021, 2:15 p.m....strengths. . Steps of an IP: 1) Protein extracts from cells or tissues contain a complex mix of... -
CRISPR-based Genome Editing Systems For Editing in E. coli
TypeBlog PostPublishedApril 13, 2021, 1:15 p.m.... plasmids for multicopy integration. (B) Anhydrotetracycline (aTc) induces expression transposases leading... -
CRISPR 101: Any Base Transversion Editors
TypeBlog PostPublishedApril 1, 2025, 1:15 p.m....Glossary Here’s a quick refresher if you ever lose track of which acronym is which. Acronym Full name ... -
Bioinformatics at Addgene
TypeBlog PostPublishedJuly 14, 2022, 1:15 p.m....data for our DNA-based reagents. Not only do we extract as much information as possible from depositing... -
Mesothelioma - Causes, Symptoms, and Treatment
TypeBlog PostPublishedDec. 13, 2016, 3:30 p.m....Addgene's Cancer Collection Pages Learn How the ClonTracer Library Can be Used to Study Cancer Drug Resistance... -
Antibiotic Resistance: An Old Solution but a New Problem
TypeBlog PostPublishedNov. 10, 2016, 3:30 p.m....today, but it doesn’t have to be some looming, abstract, convoluted scientific concept. It is imperative... -
5 Reasons to Use Reddit for Science Communication
TypeBlog PostPublishedOct. 20, 2016, 2:30 p.m....Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments Additional Resources See How ... -
Healthcare Consulting: A Door to the Business of Life Sciences
TypeBlog PostPublishedOct. 13, 2016, 2:30 p.m....roles. Healthcare consulting is a demanding but attractive choice for anyone who wants a fulfilling career... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog PostPublishedOct. 19, 2017, 1:54 p.m....proteins needed for later experiments (cellular tracking for instance) can be controlled with FLP-FRT. ... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog PostPublishedOct. 1, 2015, 2:30 p.m....examples I’m personally interested in include: intracranial self-stimulation (ICSS) and place preference... -
Plasmids 101: Control Plasmids
TypeBlog PostPublishedApril 9, 2015, 3:29 p.m...., we will take a step beyond discussion in the abstract and follow an example of a plasmid-based experiment... -
Antibodies 101: Introduction to Gating in Flow Cytometry
TypeBlog PostPublishedApril 4, 2024, 1:15 p.m.... promise! FSC and SSC: scattering To digitally extract your cells based on their size, the cytometer provides... -
Deep Mutational Scanning with One Pot Saturation Mutagenesis
TypeBlog PostPublishedFeb. 22, 2017, 3:30 p.m....labeled ssDNA is packaged into phage and then extracted. Besides taking a couple days to generate the ... -
Tips for Getting a Faculty Position
TypeBlog PostPublishedJuly 20, 2023, 1:15 p.m....focus on you and your ideas. Slides will be distracting. What is the big question? This should be...