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We narrowed to 749 results for: Tro;

Showing: 381 - 400 of 749 results

  1. Plasmid Grant to Aid Cervical Cancer Screening in Ghana

    Type
    Blog Post
    Published
    Nov. 8, 2016, 3:30 p.m.
    ...grant to order plasmids she can use as positive controls in PCR-based cervical cancer screens. This third...predominantly HPV 16 and 18.” HPV plasmids as positive controls To start her work, Gladys will improve cervical... containing a variety of HPV genes as positive controls in her PCR screens. She hopes that these screens...

  2. Grad School Advice Part 1: Picking a Lab and a Project

    Type
    Blog Post
    Published
    Sept. 1, 2016, 2:30 p.m.
    ...projects 0:28 - 1:12 : Introduction from Tyler 1:13 - 2:02 : Niroshi and Ben introduce themselves 2:03 - 3...your biggest experimental success? 31:32 - end : Outro In our next episode, Niroshi and Ben discuss how...

  3. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...parental cell line, but not in the iso-genic knockout control. This can involve cell surface labeling and/or ... Additionally, it can be a real challenge to troubleshoot RNAi failures. Potential explanations for RNAi... target of interest, you can add an additional control containing treated cells. The expected change in...55133-7 More resources from the Addgene blog Introduction to Gating in Flow Cytometry Antibodies 101: ...

  4. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    Published
    Dec. 18, 2018, 1:17 p.m.
    ...the performance of different nucleases. Nicola Patron’s lab compared the efficiency and specificity of...plasmids Anti-CRISPR proteins for optogenetic control of CRISPR-Cas9 Anti-CRISPR proteins that inhibit...has adapted anti-CRISPR proteins for optogenetic control of CRISPR-Cas9. By combining AcrIIA4, a Streptococcus...

  5. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    Published
    Nov. 13, 2015, 3:30 p.m.
    ...They kicked off their day with breakfast and an introduction to Addgene with our Executive Director, Joanne...in-depth look at some of the teams at Addgene. An introduction to plasmids with Director of Biology Lianna ...our UK team member Benoit Giquel. Bluegene’s globetrotting impressed our guests, as did how much work is...

  7. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    Published
    March 22, 2016, 2:30 p.m.
    ... they do so by activating signaling pathways controlling properties like growth, survival, and differentiation...inactivated the pathway. “The idea is that if you introduce them into cells one at a time, you could identify... for pathways that drive resistance to drugs in vitro, and they’ve shown that it works. But, he says that...

  8. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    Published
    Dec. 19, 2013, 3:46 p.m.
    ...growth based on data voluntarily made available by Medtronic via The Yale University Open Data Access (YODA...got a nine percent bump in citations for their trouble, even when all kinds of other factors, including...universities. We particularly recommend Eric Lander's intro to biology course "The Secret of Life." 10)     ...

  9. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...McClean’s lab generated an optogenetic toolkit that controls repression of gene expression in Saccharomyces...transcriptional repressor Mxi1, and an optogenetic tool that controls nuclear localization (LINuS). Blue light renders...mRNA decreased after 20 minutes in xylose (which controls expression of dCas9). The lab demonstrated the...

  10. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    Published
    Oct. 15, 2024, 1:15 p.m.
    ...for packaging: AAV1, AAV2, AAV5, AAV8, or AAV Retrograde (AAVrg) capsids.  Please be aware that this ordering...Packaged on Request prep and run the same quality control assays we run with all of our in-stock viral preps...plasmids packaged in AAV1, AAV5, AAV8, and AAV Retrograde to be 1x1013 and titers for AAV2 to be 7x1012...

  11. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ... system is toxic to proliferating cells. A Cre-estrogen receptor ligand binding domain-fusion can prevent... toxicity in proliferating Drosophila cells by estrogen-dependent activity regulation. Heidmann D, Lehner... BAC/PAC recombineering techniques. Semprini S, Troup TJ, Kotelevtseva N, King K, Davis JR, Mullins LJ...

  12. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    Published
    Oct. 7, 2014, 3 p.m.
    ... me like the postdocs are taking some steps to control their own future in science (see my previous blog...vehemently about the need for change.  Dr. Bourne strongly believes this is an amazing time of promise for... Novartis industry postdoctoral program.  His strongest comments were about the astounding lack of reproducibility...

  13. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    Published
    June 4, 2020, 1:22 p.m.
    ...and/or the use of chelating agents that can be introduced in the purification protocol to remove endonucleases... your preparation for a long time. Tris buffer controls the pH, while the EDTA chelates any divalent cations...of using a Tris buffer is that it allows better control of the pH preparation stabilizing the plasmid, ...

  14. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    Published
    June 29, 2023, 7 p.m.
    ...the workforce, but my main concerns were building strong connections with coworkers and doing something ...interested in knowing you as a person, builds a strong sense of trust between coworkers. Since I trusted...about making connections with coworkers seem, in retrospect, nonsensical. I have found the people at Addgene...

  15. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...Getting into the office for the first time, being introduced to all the members around the office one by one...and undigested samples of the clones on an electrophoresis gel in order to differentiate which clone had...Once we had gotten our results back from the electrophoresis, my manager and I were unsure if there were...

  16. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    Published
    Nov. 22, 2016, 3:30 p.m.
    ... sections of a paper, the abstract, figures, introduction, etc. so as to thoroughly explain the do’s and...reader is not in their head. The scientist has to introduce the reader to the manuscript and engage them in...to make the final product with an interesting introduction and concise discussion. Many thanks to our ...

  17. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    Published
    Nov. 1, 2016, 2:30 p.m.
    ...sequence the plasmid to confirm no mutations were introduced during PCR or any subsequent steps. Using high...DNA polymerases, I have never had any mutations introduced into my inserts. However, with any PCR-based ...PCR-based cloning technique there is a chance of introducing an unwanted mutation. Tips for high efficiency cloning...

  18. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ...recognition come from the successes of the team. A strong sense of engagement in the work Behind-the-scenes...    This is the first in a 5 part series on Introduction to Managing People for Scientists. Subscribe...follow the entire series as it is published. Good introductory reference books on managing people: Skills ...

  19. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...function of Your Gene Of Interest (YGOI) in a controlled environment under various conditions. The first...expression system and go for a lentiviral or a retroviral backbone. This is considered to be a more robust...great for overexpressing YGOI in an isolated, controlled environment. In most cases bacterial expression...

  20. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ... store a record of invading phages but to also destroy the phage upon re-exposure (2, 3). More specifically...complex binds to the foreign DNA, a cut is made to destroy the invader. Wikipedia, accessed 25 November...ultimate victor will be the species who has the strongest will to survive. Genome engineering and future...
Showing: 381 - 400 of 749 results