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Showing: 381 - 400 of 621 results

  1. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...penetration depth as well as high spatial and temporal resolution, making it ideal for visualizing gene expression...also included an analogous set of transient constitutively-expressed mARG plasmids driven by CMV promoters...

  2. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...generation of base modifiers available. This system utilizes an RNA template containing the desired edit adjoined...like with base editing, alternative Cas9s can be utilized allowing for PAM flexibility. Fig. 2: Prime...

  4. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ... processing in live cells. Indeed, groups have utilized dCas13 fusion proteins for imaging, tracking, ...global RNA expression, cell stress, and RNA size distribution. When comparing LwaCas13a to shRNA, they found...

  5. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...known functions has the potential to be used in therapeutics, as it can increase gene expression at certain..., Garnett MJ (2019) Prioritization of cancer therapeutic targets using CRISPR–Cas9 screens. Nature 568...

  6. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    .... An internal control may be a plasmid that constitutively expresses a reporter protein (e.g., GFP or ...many variables. However daunting designing and executing these steps may be, proper controls are a basic...

  7. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ... Used since the mid-90s, this technique has revolutionised the way we apprehend molecular complexes and...wavelength, to the concentration of fluorophore in solution) are two additional parameters that can affect...

  8. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...supported/readable. Is the author or their host institution expected to provide data in formats exported ...resources can offload the burden of data storage and distribution. I would argue that the journals themselves ...

  9. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ... is a nearly ubiquitous tool, but proceed with caution! Loading controls only address one type of error...by sample concentration as well as by antibody dilution — both factors we’ve covered in our previous blog...

  10. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...biotinylating your macromolecule of interest To utilize the natural binding of biotin and avidin, macromolecules...biotinylation reagents to help with the specific elution of biotinylated proteins. For help determining ...

  11. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...occurring due to protein synthesis, degradation, or reconstitution (reviewed in Shcherbakova et al., 2018). For...K.D. et al. A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters...

  12. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...interview and sell a vision for your contribution to a Department's/Institution's success is the task. Do your...

  13. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...graphing data, choosing controls, or mixing clear solutions—public speaking is skill that any scientist can...What happens next?”  Describe how you pushed to a solution or overcame obstacles. Take time to consider the...

  14. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...demonstrated two potential strategies for CRISPR-HIV therapeutics - one using dCas9-SAM to activate HIV-1 transcription...next few years, as it would open up many new therapeutic possibilities for some of the world’s most common...

  16. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ... for i-GONAD. A micropipette is used to inject solution into the oviduct wall between the ampulla and ...incision that exposes the ovaries and oviducts. After solution injection into the oviductal lumen, tweezer-type...

  17. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...modification was impossible. With the genome engineering revolution came epigenome-engineering tools - zinc finger...transduced cells. Grant Challen’s lab also created constitutive (pCMV-dCas9-D3A) and Tet-dependent (TetO-dCas9...

  18. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...them? Or maybe you just want to make some really beautiful images? Multiplex IF may be just what you need... L., Zhu, Y., Neumann, E., De Caestecker, M., Gutierrez, D., & Spraggins, J. (2022). Multiplex Immunofluorescence...

  19. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...SV40, are always active and thus referred to as constitutive promoters. Others are only active under specific...that also express GAL4. Usage of this system revolutionized genetic studies in flies. An additional component...

  20. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ... percentage of acrylamide. To further improve resolution, use a stacking gel on top of the resolving gel...Gradient gels are another great option to enhance resolution. In this setup, gels have an increasing range...
Showing: 381 - 400 of 621 results