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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 631 results for: UTY

Showing: 381 - 400 of 631 results

  1. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...track all of our 40,000 samples available for distribution!    Check Out How We Think about Developing...The preferred E. coli strains for storage and distribution at Addgene are DH5α, NEB stable, ccdb survival...

  2. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ...accurate than short-read sequencing. At Addgene, we routinely use Nanopore technology to sequence our packaged...written by Alyssa Shepard, with significant contributions from Alyssa Cecchetelli, Cary Valley, and Holly...

  3. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ... developed, though, researchers at HuidaGene Therapeutics guessed that fusing MPG to an ABE would allow...recognize T and/or C substrates. The HuidaGene Therapeutics team has deposited their editors, called gTBE...

  5. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ... processing in live cells. Indeed, groups have utilized dCas13 fusion proteins for imaging, tracking, ...global RNA expression, cell stress, and RNA size distribution. When comparing LwaCas13a to shRNA, they found...

  6. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...molecules. Using a technique called systematic evolution of ligands by exponential enrichment (SELEX), ...Peixuan AND Haque, Farzin. RNA Nanotechnology and Therapeutics. Boca Raton: CRC Press, 2014. 5. Hayat, Akhtar...

  7. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ... spatiotemporal control over its expression. Constitutive or inducible Cre recombinases can be introduced...method to dissect the individual and shared contributions of transcript isoforms to neuronal physiology...

  8. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...Cas9. Unfortunately, certain drawbacks limit the utility of these systems: St1Cas9 requires a very specific...  Wang D, Zhang F, Gao G (2020) CRISPR-Based Therapeutic Genome Editing: Strategies and In Vivo Delivery...

  10. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...generation of base modifiers available. This system utilizes an RNA template containing the desired edit adjoined...like with base editing, alternative Cas9s can be utilized allowing for PAM flexibility. Fig. 2: Prime...

  11. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...penetration depth as well as high spatial and temporal resolution, making it ideal for visualizing gene expression...also included an analogous set of transient constitutively-expressed mARG plasmids driven by CMV promoters...

  12. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...pathogen or virus detection), therapeutics and sensitive genotyping. The beauty of SHERLOCK systems is that...bottleneck. In the last few years, scientists have utilized CRISPR-Cas9 protein variants, Cas13, and Cas12a...

  14. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...known functions has the potential to be used in therapeutics, as it can increase gene expression at certain..., Garnett MJ (2019) Prioritization of cancer therapeutic targets using CRISPR–Cas9 screens. Nature 568...

  15. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    .... An internal control may be a plasmid that constitutively expresses a reporter protein (e.g., GFP or ...many variables. However daunting designing and executing these steps may be, proper controls are a basic...

  16. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ... Used since the mid-90s, this technique has revolutionised the way we apprehend molecular complexes and...wavelength, to the concentration of fluorophore in solution) are two additional parameters that can affect...

  17. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...supported/readable. Is the author or their host institution expected to provide data in formats exported ...resources can offload the burden of data storage and distribution. I would argue that the journals themselves ...

  18. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ... genes with potentially harmful mutations in therapeutic settings. Silencing of a target gene is achieved...the backbone, shRNAs are most often expressed constitutively, either in lentiviral or retroviral backbones...

  19. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ... is a nearly ubiquitous tool, but proceed with caution! Loading controls only address one type of error...by sample concentration as well as by antibody dilution — both factors we’ve covered in our previous blog...

  20. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...occurring due to protein synthesis, degradation, or reconstitution (reviewed in Shcherbakova et al., 2018). For...K.D. et al. A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters...
Showing: 381 - 400 of 631 results