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  1. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...factors. You have to weigh the pros and cons of various methods and choose what is best for you — and the...array of purposes, but mainly for the delivery of various therapies and viral vectors. This injection sounds...

  2. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...are Sf9 cells, which are derived from the pupa ovarian tissue of fall armyworms (Spodoptera frugiperda...protein production. Titration of baculovirus can vary depending on the protocol used. Plaque assays are...

  3. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...destabilizing domains which will be identified by various enzymes and sent to the proteasome for degradation...protein levels aren’t limited to ‘on’ and ‘off’; varied levels of drug concentration can yield intermediate...

  4. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...viral genome in eukaryotic cells.1,2 Since then, a variety of IRES sequences have been discovered - many from... reporter gene). Addgene's collection offers a variety of plasmids to express two or more genes, some ...

  5. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...generating single or multi-gene knockouts in a wide variety of cell types, gene editing using homology directed...performing CRISPR experiments using RNPs is the variety of methods that can be used to deliver a Cas9-gRNA...

  6. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ... antibodies, frequently of the mouse or rabbit variety. While antibodies certainly aren’t “broken,” their...that nanobodies come in 1x and/or a 3x cysteine varieties. This refers to the number of cysteine sites the...

  7. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...we just made the sample bigger? In ExM, and its various derivatives, biological samples are enmeshed in...Damstra, et al. 2022; Yu, et al. 2020). With any variation, your samples can tear or expand unevenly, introducing...

  8. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...between genomes, and have been adapted for a wide variety of applications - as mutagenesis tools for genetic..., 2020). VchINT was also shown to be active in various gram-negative bacterial hosts of interest, including...

  10. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...SaCas9. As seen with Cas9 orthologs, Cpf1 efficiency varies widely with gRNA sequence. Both groups used multiple...Cpf1 is also sensitive to single mismatches, but variably so, with Kleinstiver et al. reporting that Cpf1...

  11. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...us to express the CRISPR complex much easier in various cells, including primary mouse cells. We believe...will allow researchers to use enChIP in a wide variety of biological systems. We are currently developing...

  12. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...within each color also exist with only slight variations separating them.  Please note that many fluorescent...fluorescent biosensors has been designed to detect a variety of intracellular conditions, including ion (such...

  13. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...collection of lentiviral plasmids created for a variety of applications including cDNA expression, shRNA-mediated...breaks down the biosafety concerns associated with various aspects of lentiviral transduction along with suggestions...

  14. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ...researchers feel that they lack formal training in a variety of transferable skills. At Addgene we've set out...indeed.com or monster.com, where you’ll find a variety of listings. Post your CV and make it visible, ...

  15. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ... able to quickly synthesize information from a variety of fields in order to provide useful solutions ...been a long brewing frustration for me. I read a variety of psychology, business, personnel, and sociology...

  16. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ... participate in forum discussions about a wide variety of topics. Individual forums are hosted on subreddits...sometimes, civil. r/Science in particular hosts a variety of conversations with people who work in many different...

  17. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...levels (Mauro and Chappell, 2014). Due to this variation in tRNA expression, the codons that encode a protein...3645–3650 https://doi.org/10.1073/pnas.0909910107 Varani G, McClain WH (2000) The G·U wobble base pair. ...

  18. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...Since the efficiency of CRISPR/Cas9 genome editing varies across targets, they tested two genes in mice. ...Resources on the Addgene Blog Learn more about Cas9 Variants and the Expanded CRISPR Toolbox Read about CRISPR...

  20. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...target genomic locus/loci, choose a base editor variant from Table 1 depending on the PAM sequence. Avoid...Expanding the base editing scope in rice by using Cas9 variants." Plant biotechnology journal (2018). PubMed PMID...
Showing: 381 - 400 of 633 results