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We narrowed to 642 results for: Vars;

Showing: 381 - 400 of 642 results

  1. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    Published
    Aug. 6, 2015, 2:30 p.m.
    ...postdoctoral issues first at Brigham and Women’s Hospital/Harvard Medical School and then Boston University (institutions...them. As a committee member, I volunteered for various tasks as things came up. For example, I updated...

  2. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...recommend the use of an electronic pipettor with variable spacing (such as the Viaflo from Integra Biosciences... clones. The technique is applicable to a wide variety of cell lines, from HEK to CHO cells to neural ...

  4. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ...human disease. However, the rate of transfer was variable. They measured transfer rates by comparing the... of a constitutely expressed RFP reporter, was variable across bacterial strains, ranging from an ~8 to...

  5. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ...researchers feel that they lack formal training in a variety of transferable skills. At Addgene we've set out...you want to be!  References Becoming the boss. Harvard Business Review, January 2007. Three Rules for ...

  6. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...stick out to form the “arms” holding the highly variable regions that bind to specific antigens. In contrast... Cloning System for Characterization of Enzyme Variants. In: Methods in Molecular Biology. Humana Press...

  7. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...improved kinetics and responsivity in screening ASAP3 variants. B) Electrical and optical recordings of spontaneous...assay dozens of GPCRs across many cell types in varying conditions, revealing context-dependent responses...

  8. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...factors. You have to weigh the pros and cons of various methods and choose what is best for you — and the...array of purposes, but mainly for the delivery of various therapies and viral vectors. This injection sounds...

  9. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...are Sf9 cells, which are derived from the pupa ovarian tissue of fall armyworms (Spodoptera frugiperda...protein production. Titration of baculovirus can vary depending on the protocol used. Plaque assays are...

  10. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...destabilizing domains which will be identified by various enzymes and sent to the proteasome for degradation...protein levels aren’t limited to ‘on’ and ‘off’; varied levels of drug concentration can yield intermediate...

  11. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...viral genome in eukaryotic cells.1,2 Since then, a variety of IRES sequences have been discovered - many from... reporter gene). Addgene's collection offers a variety of plasmids to express two or more genes, some ...

  12. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...generating single or multi-gene knockouts in a wide variety of cell types, gene editing using homology directed...performing CRISPR experiments using RNPs is the variety of methods that can be used to deliver a Cas9-gRNA...

  13. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ... antibodies, frequently of the mouse or rabbit variety. While antibodies certainly aren’t “broken,” their...that nanobodies come in 1x and/or a 3x cysteine varieties. This refers to the number of cysteine sites the...

  14. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...between genomes, and have been adapted for a wide variety of applications - as mutagenesis tools for genetic..., 2020). VchINT was also shown to be active in various gram-negative bacterial hosts of interest, including...

  15. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...we just made the sample bigger? In ExM, and its various derivatives, biological samples are enmeshed in...Damstra, et al. 2022; Yu, et al. 2020). With any variation, your samples can tear or expand unevenly, introducing...

  17. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...SaCas9. As seen with Cas9 orthologs, Cpf1 efficiency varies widely with gRNA sequence. Both groups used multiple...Cpf1 is also sensitive to single mismatches, but variably so, with Kleinstiver et al. reporting that Cpf1...

  18. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...us to express the CRISPR complex much easier in various cells, including primary mouse cells. We believe...will allow researchers to use enChIP in a wide variety of biological systems. We are currently developing...

  19. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...within each color also exist with only slight variations separating them.  Please note that many fluorescent...fluorescent biosensors has been designed to detect a variety of intracellular conditions, including ion (such...

  20. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...collection of lentiviral plasmids created for a variety of applications including cDNA expression, shRNA-mediated...breaks down the biosafety concerns associated with various aspects of lentiviral transduction along with suggestions...
Showing: 381 - 400 of 642 results