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We narrowed to 906 results for: addgene

Showing: 381 - 400 of 906 results

  1. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...article can be obtained through Addgene: Rapamycin-inducible Cas9 sets (Addgene plasmids 62883 &62884; 62885...At Addgene we're continually impressed with the amazing plasmid technologies developed by our community... Rapamycin-inducible dCas9-VP64 activator set (Addgene plasmids 62887 & 62888) Zetsche et al., Nat ...brilliant SunTag system plasmids are available through Addgene and are already very popular. Use these plasmids...pathways (Figure 2). They are all available now at Addgene. 1 Lungu et al., Chem Biol. 2012 Apr 20;19(4..., and other plasmids from this article through Addgene. Nihongaki et al., Chemistry and Biology. 2015...human genes. The plasmids are all available at Addgene ready to light up the genome of your favorite cells...

  2. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ... & Small Molecule Fluorophores. Addgene Blog. https://blog.addgene.org/better-dyeing-through-chemistry-small-molecule-fluorophores... is now the most popular self-labeling tag in Addgene’s collection. CLIP-tag: Also in 2008, Kai Johnsson...are the most popular self-labeling enzymes in Addgene’s collection, so you may be able to get a plasmid...ligand to use for your experiment, and explore Addgene's collection to find self-labeling fusion proteins...10.1021/acs.biochem.1c00258 More resources from Addgene Better Dyeing Through Chemistry Illuminating ...GCE4All: Making Genetic Code Expansion Accessible Addgene's Genetic Code Expansion Collection ...

  3. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ... and references Resources on addgene.org Addgene's CRISPR Guide Addgene's CRISPR 101 eBook CRISPR resources...nearby genes (Figure 1A-C). Find CRISPR plasmids at Addgene! Knock-in: Introducing tags or stop signals Once...resources More resources on the Addgene blog RNA Editing with Cas13 Targeting RNA with Cas13a (C2c2) Cas13d...

  4. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ...partner with Addgene. What are the most important issues for our partners and how can Addgene work with ...training in a variety of transferable skills. At Addgene, we've set out to fill this gap by highlighting...Richard D. Lewis Additional resources on the Addgene blog More from the transferable skills guide: ...management Managing a team Leadership Resources on Addgene.org Check out our careers page Watch our career ...

  5. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...conferences Resources on the Addgene website Become of a member of the Addgene depositor community Learn... science communication.       Resources on the Addgene blog Read other posts on scientific communication...Learn about careers at Addgene ...

  6. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...informed this section.  Find mStayGold plasmids at Addgene here!   Ando, R., et al. (2023). StayGold variants...primate brains with brilliance By Hridaya Patel (Addgene co-op) Researchers in the Gradinaru lab have developed...good option for essentially all applications. Addgene’s plasmid collection includes over 10,000 plasmids...both of which are also available in trial sizes. Addgene recommends these recombinant antibodies for use...

  7. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ... Resources on Addgene.org Addgene’s Viral Services Addgene’s Virus Protocols Addgene’s Chemogenetics ...Chemogenetics Guide Resources on the Addgene blog Viral Vectors 101: Chemogenetics Viral Vectors 101: Optogenetics...

  8. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ... do not necessarily represent the views of Addgene. Addgene performs Sanger sequencing on select regions...Twitter - Tweet suggestions and tips with #NgAgo The Addgene Blog - Leave suggestions and tips in the comments...PubMed Central PMCID: PMC4343198. Resources on the Addgene Blog Learn More about NgAgo Enhance CRISPR Targeting...comments section below or contact blog@addgene.org if you'd like to write a more detailed post It may take...Cpf1 Stacks Up to Cas9 and NgAgo Resources on Addgene.org Request NgAgo Find CRISPR Resources Catch Up...

  9. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...where the gene is expressed. Click here to view Addgene’s collection of empty backbones for constructing... such as FRET, calmodulin, and others. Review Addgene's collection of fluorescent biosensors here. Optogenetics...OpenOptogenetics and find optogeneic actuators and sensors at Addgene. Cell marking/selection: Expression constructs ...microscopy. Read More: All Plasmids 101 Articles Addgene's Fluorescent Protein Guide Note: A. Max Juchheim...

  10. Antibody Data Repositories and Search Engines

    Type
    Blog Post
    Published
    Oct. 31, 2024, 1:15 p.m.
    ...Human Cell Differentiation Molecules group Addgene Data Hub Any (see notes) Any Data repository...repository Only accepts data for antibodies in Addgene’s repository  IBEX Knowledge-Base  Any IBEX...

  11. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ...’re bringing you a brand new third edition of Addgene’s CRISPR 101 eBook. This new edition of the eBook...CRISPR nuclease. Note: Most CRISPR plasmids from Addgene produce either a gRNA, a nuclease, or both. Applications...

  12. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...the biotin ligase BirA backbone (Eric Campeau, Addgene plasmid #26624) was unsuccessful. Nothing more ...pMal-T-Avi-His/BirA plasmid now available through Addgene. We have successfully used this plasmid to biotinylate...cell signaling.     Additional Resources on the Addgene Blog Check out other Hot Plasmids Find Additional...Posts on Plasmid Technologies Resources at Addgene.org Learn about Popular Plasmids in the Repository...

  13. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...These vectors listed below should be released at Addgene in the next 6 months, pending quality control. ....1-WPRE-SV40  Additional resources on the Addgene blog Read all of our viral vectors blog posts ...item is available for order please email help@addgene.org with the vector name and serotype you’d like ...

  14. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...For example, the many resources available on the Addgene website didn't come into being through the actions... Managerial Skill" Additional Resources on the Addgene Blog Read the Rest of the Management for Scientists... Individual Development Plan Resources on the Addgene Website Are You a Molecular Biologist? Find Plasmids...

  15. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    Published
    Aug. 28, 2014, 3:34 p.m.
    ...received matches the expected sequence. Here at Addgene, we use NGS-based quality control to confirm the...necessary but also an easy process. Please vist Addgene's resource for plasmid verification to find additional...DNA gel.  More Plasmid Eductional Resources: Addgene's Molecular Biology Reference Find More Protocols...

  16. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...contributing to the Addgene Antibody Data Hub.  Figure 5: Immunohistochemistry with Addgene's Anti-APP [...Immunohistochemistry (Human). Addgene Report, doi: https://doi.org/10.57733/addgene.6ncijt.  ...

  17. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...resources on addgene.org Addgene's Antibody Guide Addgene's Ready-to-Use Antibody collection Addgene's Recombinant...R2 never dip below 0.95! More resources on the Addgene blog Antibodies 101: ELISAs The Four ELISAs and...

  18. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...synthetic? How Addgene;s dataset reveals trends in biological innovation Resources on Addgene.org Check out...silence neurons in vivo (Slimko and Lester, 2003).  Addgene has a large selection of codon optimized genes,...embo-reports/kvd001 Additional resources on the Addgene blog Read more codon usage bias in Plasmids 101...out Addgene's Molecular Biology Reference Read about genetic code expansion Browse our plasmid collection...

  19. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...ordering plasmids containing them from Addgene. If a plasmid at Addgene contains a gene that’s been codon ... for example). As many plasmids available from Addgene now have full sequence data, we recommend directly...PMCID: PMC2840511. Additional resources on the Addgene blog Plasmids 101: protein expression Plasmids...Plasmids 101 featured topic page Resources on Addgene.org Molecular biology reference pages Genetic code...

  20. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...resources on the Addgene blog: Introduction to Immunofluorescence View all of Addgene's antibody posts  ...Resources on Addgene.org: Find plasmids encoding recombinant antibodies at Addgene  ... found this blog helpful be sure to check out Addgene’s blog for other antibody topics.     References...
Showing: 381 - 400 of 906 results