We narrowed to 526 results for: arl

1. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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   Blog Post

   Published

   April 23, 2018, 2 p.m.

   ...and quantitative analysis of epigenetic memory at nearly any locus. The FIRE-Cas9 plasmids can be found ...

2. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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   Blog Post

   Published

   April 13, 2017, 2:30 p.m.

   ...than tracking generic autophagy markers. It’s particularly useful for comparing bulk autophagy vs. mitophagy...

3. Human Germline Editing Using CRISPR

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   Blog Post

   Published

   Aug. 10, 2017, 2:19 p.m.

   ... appealing for heterozygous mutations, it will clearly be much more challenging for homozygous mutations...

4. Lighting Up Cell Signaling with Photoswitchable Kinases

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   Blog Post

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   July 25, 2017, 1:20 p.m.

   ...versions of MEK1, MEK2, Raf1, and CDK5 all behave similarly to their endogenous counterparts. Additionally...

5. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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   Blog Post

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   Sept. 4, 2018, 12:53 p.m.

   ...promoters created by Segall-Shapiro et al. showed nearly no position-dependent differences in gene expression...

6. RaPID Detection of RNA-protein Interactions

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   Blog Post

   Published

   Aug. 16, 2018, 12:40 p.m.

   ...characterized by increased iron serum levels and early-onset cataracts, is characterized by point mutations...

7. Seven Tips for Using LinkedIn as a Scientist

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   Blog Post

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   Oct. 1, 2019, 1:21 p.m.

   ...to send invitations. Finally, if you don’t know clearly why you are inviting someone to connect, perhaps...

8. Troubleshooting Your Plasmid Cloning Experiment

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   Blog Post

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   Sept. 24, 2019, 12:55 p.m.

   ...having difficulties in amplifying a template, particularly from a large genome, you can recheck your primer...

9. Antibodies 101: Conjugation

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   Blog Post

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   March 26, 2024, 1:15 p.m.

   ...touched on fluorophores as antibody conjugates in an earlier post, where you can find some additional tips for...

10. Giving gRNAs a Facelift - Synthetic and Beyond

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    Blog Post

    Published

    Oct. 20, 2022, 1:15 p.m.

    ...et al., Frontiers in Genome Editing). This is particularly important in experiments in which viability ...

11. Developing Lab Management Software for Biology

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    Blog Post

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    Aug. 25, 2016, 2:30 p.m.

    ...things will trip everyone up at some point, decide early on what to do in a namespace collision. It may even...

12. Gendered Innovations: Why Does Sex of the Cell Matter?

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    Blog Post

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    July 21, 2016, 2:30 p.m.

    ... important point, however, is that cost at this early stage of research is minimal compared to that of...

13. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

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    Blog Post

    Published

    April 12, 2016, 2:30 p.m.

    ...Sciences of the University of Hong Kong. He is particularly interested in developing and applying new technologies...

14. Addgene's Tips for Plasmid Quality Control

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    Blog Post

    Published

    Jan. 14, 2016, 3:30 p.m.

    ...have a few tricks to simplify the process. First, nearly all of our Sanger sequencing is done from glycerol...

15. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

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    Blog Post

    Published

    Dec. 16, 2015, 3:30 p.m.

    ...SpCas9-HF1 Kleinstiver et al and the Joung Lab similarly thought that if they could weaken sequence independent...

16. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

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    Blog Post

    Published

    Nov. 24, 2015, 3:30 p.m.

    ...and CFP can activate certain T-DDOG constructs similarly to GFP, but commonly used red fluorescent proteins...

17. New Tool for Lineage Tracing: The ClonTracer Library

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    Blog Post

    Published

    Sept. 22, 2015, 2:30 p.m.

    ...Vivo Pharmacology at Novartis Oncology and is particularly interested in applying what we have learned ...

18. 5 Steps to Writing Grant Proposals for Grad Students

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    Blog Post

    Published

    Feb. 11, 2014, 12:13 p.m.

    ...that the goal and the impact of the project are clearly stated and align with the description of the RFA...

19. Hot Plasmids February 2024

    Type

    Blog Post

    Published

    Feb. 20, 2024, 2:45 p.m.

    ...Cas12-RNP-PAGE achieved high editing frequency (nearly 100% in some cases) and minimal toxicity, including...

20. Viral Vectors 101: Calcium Sensors

    Type

    Blog Post

    Published

    Aug. 10, 2023, 1 p.m.

    ... aims and requirements of in vivo studies. A particularly useful sensor for neuroscientists is CaMPARI...