We narrowed to 529 results for: arl

1. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

   Type

   Blog Post

   Published

   Sept. 4, 2018, 12:53 p.m.

   ...promoters created by Segall-Shapiro et al. showed nearly no position-dependent differences in gene expression...

2. RaPID Detection of RNA-protein Interactions

   Type

   Blog Post

   Published

   Aug. 16, 2018, 12:40 p.m.

   ...characterized by increased iron serum levels and early-onset cataracts, is characterized by point mutations...

3. dTAG - You're it!

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   Blog Post

   Published

   June 21, 2018, 2:06 p.m.

   ...proteasome machinery to degrade any protein. We are particularly excited about the dTAG system as we can use ...

4. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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   Blog Post

   Published

   April 23, 2018, 2 p.m.

   ...and quantitative analysis of epigenetic memory at nearly any locus. The FIRE-Cas9 plasmids can be found ...

5. Human Germline Editing Using CRISPR

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   Blog Post

   Published

   Aug. 10, 2017, 2:19 p.m.

   ... appealing for heterozygous mutations, it will clearly be much more challenging for homozygous mutations...

6. Lighting Up Cell Signaling with Photoswitchable Kinases

   Type

   Blog Post

   Published

   July 25, 2017, 1:20 p.m.

   ...versions of MEK1, MEK2, Raf1, and CDK5 all behave similarly to their endogenous counterparts. Additionally...

7. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

   Type

   Blog Post

   Published

   April 13, 2017, 2:30 p.m.

   ...than tracking generic autophagy markers. It’s particularly useful for comparing bulk autophagy vs. mitophagy...

8. Seven Tips for Using LinkedIn as a Scientist

   Type

   Blog Post

   Published

   Oct. 1, 2019, 1:21 p.m.

   ...to send invitations. Finally, if you don’t know clearly why you are inviting someone to connect, perhaps...

9. Troubleshooting Your Plasmid Cloning Experiment

   Type

   Blog Post

   Published

   Sept. 24, 2019, 12:55 p.m.

   ...having difficulties in amplifying a template, particularly from a large genome, you can recheck your primer...

10. Giving gRNAs a Facelift - Synthetic and Beyond

    Type

    Blog Post

    Published

    Oct. 20, 2022, 1:15 p.m.

    ...et al., Frontiers in Genome Editing). This is particularly important in experiments in which viability ...

11. What's New in CRISPR - November 2021

    Type

    Blog Post

    Published

    Nov. 18, 2021, 2:15 p.m.

    ...Building upon the PE2 and PE3 systems of their earlier work, they developed a system in which the cellular...

12. Antibodies 101: Polyclonal Antibodies

    Type

    Blog Post

    Published

    July 14, 2021, 1:15 p.m.

    ...increased sensitivity, polyclonal antibodies are particularly useful for certain research applications. Many...

13. Antibodies 101: Conjugation

    Type

    Blog Post

    Published

    March 26, 2024, 1:15 p.m.

    ...touched on fluorophores as antibody conjugates in an earlier post, where you can find some additional tips for...

14. Developing Lab Management Software for Biology

    Type

    Blog Post

    Published

    Aug. 25, 2016, 2:30 p.m.

    ...things will trip everyone up at some point, decide early on what to do in a namespace collision. It may even...

15. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type

    Blog Post

    Published

    July 21, 2016, 2:30 p.m.

    ... important point, however, is that cost at this early stage of research is minimal compared to that of...

16. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type

    Blog Post

    Published

    April 12, 2016, 2:30 p.m.

    ...Sciences of the University of Hong Kong. He is particularly interested in developing and applying new technologies...

17. Addgene's Tips for Plasmid Quality Control

    Type

    Blog Post

    Published

    Jan. 14, 2016, 3:30 p.m.

    ...have a few tricks to simplify the process. First, nearly all of our Sanger sequencing is done from glycerol...

18. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type

    Blog Post

    Published

    Dec. 16, 2015, 3:30 p.m.

    ...SpCas9-HF1 Kleinstiver et al and the Joung Lab similarly thought that if they could weaken sequence independent...

19. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type

    Blog Post

    Published

    Nov. 24, 2015, 3:30 p.m.

    ...and CFP can activate certain T-DDOG constructs similarly to GFP, but commonly used red fluorescent proteins...

20. New Tool for Lineage Tracing: The ClonTracer Library

    Type

    Blog Post

    Published

    Sept. 22, 2015, 2:30 p.m.

    ...Vivo Pharmacology at Novartis Oncology and is particularly interested in applying what we have learned ...