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TypeBlog PostPublishedFeb. 25, 2020, 2:15 p.m....genetic code of living organisms and its societal implications even before the discovery of genes themselves... that revolves around synthetic humans called replicants which are genetically engineered by a powerful...us to always consider the ethics and broader implications of our research. Want the Genome Editing Trading...
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Prime Editing: Adding Precision and Flexibility to CRISPR Editing
TypeBlog PostPublishedJan. 13, 2025, 9:49 p.m....editing typically needs to be optimized for each application, and the efficacy varies widely — it still almost...editing variants Four years after their initial publication, the Liu lab returned to the reverse transcriptase...Image from Doman et al., 2023, under a CC BY 4.0 license. epegRNAs: Structured RNAs with improved ... -
Antibody Validation for Flow Cytometry
TypeBlog PostPublishedSept. 10, 2024, 1:15 p.m....that the antigen being detected in the intended application — here, flow cytometry — is the desired target...quantification of antibody performance in major research applications. eLife, 12, RP91645. https://doi.org/10.7554...of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72. eLife... -
New Antibodies in the Lab Animation!
TypeBlog PostPublishedNov. 19, 2024, 2:15 p.m....Thinking about using antibody-based applications in your experiment...but not sure where to start or ...or quick refresher on — these common antibody applications. Thanks for watching, and we hope you found... -
The Codon Guide to Synthetic Biology
TypeBlog PostPublishedSept. 14, 2023, 1:15 p.m....from Bruce Wetzel and Harry Schaefer, NCI, NIH | License I’m updating it regularly as people send me suggestions...synthetic biology, DNA assembly, and various applications. I think these papers are good starting points... -
New Videos: Addgene Lab Tips
TypeBlog PostPublishedFeb. 15, 2018, 2:41 p.m....trick. Just flip, and press! Lab tip #2: Tube flick trick When combining reagents in the lab, it is ...the various reagents to a single tube and simply flick the tube several times. This will help ensure ensure... -
Antibody Data Repositories and Search Engines
TypeBlog PostPublishedOct. 31, 2024, 1:15 p.m.... specificities, from target-specific ones to application-specific ones to anything goes. We've shared ...repositories and search engines Website Targets Application Purpose Notes Antibodypedia Any Any... -
Call for Guest Bloggers
TypeBlog PostPublishedMarch 2, 2016, 3 p.m....minipreps? If so, we’d love to have you write for us! Click Here to Contribute to the Addgene Blog Possible ...molecular biologist Tips for protein purification Replica plating Anti-CRISPR Retroviral vectors Career development... -
Summer Fun at Addgene!
TypeBlog PostPublishedMay 12, 2022, 1:15 p.m.... Dives” series comes in! We’ll take a common application or concept and go beyond the basics - diving ...you’re ready for the next steps on some common applications, join us as we get into the weeds on some of... -
Editor's Choice, July 2016
TypeBlog PostPublishedAug. 5, 2016, 3 p.m....target and use FRET flurophores for a variety of applications. While editing this post, I learned a lot about...in their own research. All posts from July 2016 Click on the links below to read all of our excellent ... -
Synthetic Biology, Artificial Chromosomes, iGEM & More - Interview with Tom Ellis
TypeBlog PostPublishedNov. 29, 2017, 2 p.m....foundational research, training, startups, and real applications of SynBio. Chapter 3: Synthetic biology as the...synthetic genome project and constructing ever more complicated biological devices. Chapter 6: - Making research... -
Troubleshooting and Optimizing a Western Blot
TypeBlog PostPublishedSept. 17, 2024, 1:15 p.m....will simply load an extra ladder or technical replicate (ie, leftover sample) to fill the relevant wells...using an antibody validated for your protein and application of interest or validating an antibody yourself...lysis Add sonication step to protein lysis Splice variants Check literature Posttranslational... -
Designer PUF Proteins for Any RNA Target
TypeBlog PostPublishedJune 17, 2014, 7:57 p.m.... lend them many other functions for use in RNA splicing, imaging, and more. Abil says she is currently...says the new tool might even find therapeutic application in addition to their use in basic biology, for... -
Developing Transferable Skills During Science Training
TypeBlog PostPublishedJune 3, 2014, 2:14 p.m.... (be on a speaker committee) Follow industry publications like FierceBiotech to “learn the business” Attend...Academic Solos to Industrial Symphonies by Gwen Acton, Alicia Gómez-Yafal & Emily Walsh The Hard Truth About ... -
Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors
TypeBlog PostPublishedMarch 17, 2021, 2:40 p.m....where I foster dogs and help screen dog adoption applications. My most recent foster dog was with us for two...on the advisory board for Art in Giving, a 501c3 public charity raising funds for childhood cancer through... -
Modulate the Activity of 17 Signaling Pathways with One Kit!
TypeBlog PostPublishedMarch 22, 2016, 2:30 p.m.... of 17 signaling pathways that are frequently implicated in cancer cell proliferation, survival, differentiation... make the library usable for pooled screens. Applications of the cancer pathways ORFs kit Wood and his... -
Inside Addgene's 10 Year Anniversary Gala
TypeBlog PostPublishedJuly 3, 2014, 3:48 p.m....complete with all the fixings), as well as plenty of delicious desserts. We enjoyed a video highlighting the ...course of scientific research. What was once a complicated process of requesting and sharing plasmids has... -
Easing the Protein Purification Process with pCri
TypeBlog PostPublishedJune 19, 2015, 3:08 p.m....introduction to pCri, please see the original publication cited below. pCri plasmids are available either...comprehensive review of affinity tags and microbial applications." Biotechnology journal 7.5 (2012): 620-634.... -
Science as Art
TypeBlog PostPublishedMarch 2, 2015, 3:13 p.m.... new method to explore the inner structures of delicate plants and other organisms without destroying ...competition. This competition has gained so much public interest for its stunning images that is has been... -
SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture
TypeBlog PostPublishedAug. 13, 2014, 3:49 p.m.... if there are forces acting on them. For the application we were looking at, magnetic isolation of circulating...out the cell capture system.) Cancer and other applications Addgene: What does this technology mean in the...