...career Developmental biology caught me early on: a simple egg is packed with information. I am fascinated...individual diversity to get from gamete to a visibly complex - and unique - adult. My initial research directions...communication professionals who are thinking about, for example, how to use new technologies to reach the right...the world of scholarly publishing. And see for example the Eighth International Congress on Peer Review...

...repair template plasmid to create fluorescent and 3xFLAG-tag fusion proteins. The repair template and Cas9...The data are clear - the SEC-based system is both simple to use and highly efficient for C. elegans genome...elegans protein. The his-72 locus is shown as an example. Used with permission from the Genetics Society...

...Zalatan JG, et al.'s recent paper, "Engineering Complex Synthetic Transcriptional Programs with CRISPR ... the eukaryotic cell for the execution of many complex cellular behaviors, such as differentiation or ...customizable single guide RNA (sgRNA) that bears complementarity to a 20bp target sequence (Qi et al. 2013)..... (2013). PubMed. Zalatan, J. G. Engineering Complex Synthetic Transcriptional Programs with CRISPR ...

...reason is that I knew that the mind-set of the employer was not such that he/she would be looking at where...have done?” and “Would their academic experience compliment this sales and marketing role?”. Studying for... out more than necessary for my interview. The simplest place I could think of to start was “Marketing...about marketing would be to start reading a broad simple overview, learn some jargon and key phrases and...

...discussion. The Forced Choice Analysis is a fun example and you can find it in our Mentoring for Scientists...on setting SMART goals using a Development Plan template. A Development Plan or mentoring journal will ...help the Mentee feel a sense of accountability to complete action items and make progress. To illustrate ...with regular accountability checks. Focus on implementation of your goals and respect the time your Mentor...

...ul) samples of EGFP-expressing AAV packaged in various serotypes. This collection is now complete! pAAV-CAG-GFP.... Likewise, a lot of scientists have requested samples of AAV for testing purposes. To address this, we... kit with all the serotypes, since these 20 ul samples are distributed individually. See our Serotype ...

...formation. The quest for plant compounds to treat complications of diabetes Gloria Otito Izu, PhD Candidate...nephropathy, one of the major pathophysiological complications of diabetes mellitus. Recombinant human proteins...Now he aims on tracking pollution in rivers by employing synthetic biology resources and microfabrication...robot that will “swim” in the river, take water samples at specific locations, measure fluorescent signals...

...thinner membrane. Procedure The stain is a pretty simple process. First, flood the heat-fixed smear with...or pink, as shown in the image below. An example of Gram positive (purple) and Gram negative (red...is added, it interacts with the CV+, creating a complex within the peptidoglycan layer, staining the cell...the peptidoglycan layer, trapping the large dye complex created by the interaction of CV+ and I- in the...

...genome editing.  For a few reasons, Cpf1 is a simplified system for editing multiple targets compared ...s ability to process its own pre-crRNA arrays simplifies the crRNA cloning process. For cloning, Zetsche...in one direction. See the diagram below for an example of how this works. Make sure to order 5’ phosphorylated...latest developments in CRISPR technologies.  It’s a simple and effective method with multiple applications...

...Anti-FLAG frankenbody (FB) construction from the complementarity determining region of an anti-FLAG scFv and... the tag can recruit multiple frankenbodies to amplify the signal.  Liu et al. Genes Cells. 2021. https...genes within a single cassette provides increased simplicity for combinatorial genetic screens, and this optimized...Penn State Protein Ladder  by: Rachel Leeson This simple, inexpensive plasmid-based ladder system is a cost-effective...

...scientists around the world. In 2004, that was a fairly simple goal… and we had no idea how much our understanding...Steps to clear writing Let’s use Figure 1 as our example. Remember, our goal is to use words to describe...Gradient Ultracentrifugation. The reader may be, for example, a graduate student using viral vectors for the...process, try writing your own alt text for the example image. We’ll wait!  Done? Excellent! We asked our...

...at their core they can all be boiled down pretty simply – they are systems that measure biological phenomena...present in the system at a given point in time. For example, if ATP was the molecule of interest (analyte),...depending on the biology you are studying. For example, calcium dynamics in the cytosol and sarcoplasmic...all have specific sensors designed for them. For example, this class includes tension monitors associated...

...researchers new to GCE aren’t able to successfully complete their experiments and many others are dubious ...’t give it a try. Optimization Why is GCE so complicated? Well, while the techniques are straightforward...has “10x the impact of PCR. But it’s 100x more complicated.” And Ryan Mehl would know. He’s the director...universal techniques that researchers can easily implement in their own labs. They provide resources, knowledge...

...system. This common optogenetic technique can be employed in other split systems where light is required...CRISPR-based gene editing (see table below). For example, the far-red light-activated split-Cas9 (FAST) ...proteins by fusing Cas9 to photoreceptors. One prime example was from the lab of Michael Lin, who developed ...Systems.  Listen to the anti-CRISPR podcast segment Simply expressing anti-CRISPR proteins does not provide...photocage CRISPRs The aforementioned techniques each employed a photoactive strategy which had been engineered...

...only viable option for compliance. However, 10 days prior to the implementation date, our data indicated... had been collecting data and documenting the compliance of universities with the FLSA in our online resource...conditions for junior scientists but are not implementing consensus recommendations, even when a federal...

...minutes of inactivating the CASANOVA complex, fluorescent CRISPR complexes were observed binding telomeric ...using blue light (Bubeck et al., 2018). They accomplished this by inserting a photo-sensitive LOV2 domain...2015) or ratiometric Bimolecular Fluorescence Complementation (rBiFC, Grefen and Blatt, 2012). The 2in1 Plasmid...

...community is loving it. Almost 10,000 viral vector samples were distributed in 2018 and we are poised for ...unsolicited praise: “Addgene, for one, is a sterling example of collaboration and innovation between legal and...often focus on the excitement of what we are accomplishing and what more we might do. I am grateful every...supportive culture, we are attractive to the local employee pool and we get a lot of successful candidates...

...high-intensity light for activation which requires implantation of fiber-optic cables into the brain. ...were functional and their measured photocurrent amplitude, wavelength sensitivity, and off-kinetics correlated...fiber-optic cannulas were still stereotaxically implanted into the mice’s brains so that light could reach... brain may still require fiber-optic cannula implantation, but altering ChRgers so they can be activated...

...These properties allow for efficient signal amplification, making biotin conjugated antibodies good options...Fc-binding domain (Figure 1).  Figure 1: Examples of sites on antibodies used for conjugation. 1...from antibody to antibody or batch to batch. For example, take lysine conjugation, which is commonly used...reaction or the functionality of the conjugate. For example, Addgene’s antibodies are provided in a buffer ...

...indicate the ITR and serotype, respectively. For example, AAV2/8 has a type 2 ITR and a type 8 serotype....conventional single-stranded AAV (ssAAV) and a self-complementary AAV (scAAV) vector. Full-length ssAAV vector...2.5Kb) and you need expression in a hurry, self-complementary AAV (scAAV) may be an option (McCarty et al.... E. Monahan, and Richard J. Samulski. "Self-complementary recombinant adeno-associated virus (scAAV) vectors...