We narrowed to 430 results for: sequence
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TypeBlog PostPublishedAug. 11, 2016, 2:23 p.m....composition of the viral vector and the repeat sequences present in the LTR, the vector may undergo genomic...
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Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog PostPublishedJune 22, 2017, 2:30 p.m.... use your fluoresent protein fused to a signal sequence that traffics the fluorescent protein to your ... -
Hot Plasmids February 2024
TypeBlog PostPublishedFeb. 20, 2024, 2:45 p.m....tags we know and love, the FLAG tag (amino acid sequence DYKDDDDK) may be one of the most versatile. Whether... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog PostPublishedMay 18, 2021, 1:15 p.m....(A) SARS-CoV-2 RBD DMS Library generation and sequencing. From Starr et al Cell., 2020. (B) Mapping antibody-escape... -
Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog PostPublishedJune 25, 2020, 1:15 p.m....currently wrapping up my co-op with Addgene as a DNA Sequencing Analyst intern. As a member of the Quality Control... -
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins
TypeBlog PostPublishedJune 18, 2020, 1:15 p.m....pool (usually 10-30) of sybody candidates are sequenced for identification. All unique sybodies can be... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog PostPublishedMay 7, 2020, 1:15 p.m.... combination with a synthetic library of TadA sequences to create the eighth generation of adenine base... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog PostPublishedMay 15, 2014, 3:33 p.m.... color and application Almost as soon as its sequence was elucidated, scientists began engineering new... -
Fluorescent Proteins 101: Aptamer Fluorophores
TypeBlog PostPublishedApril 11, 2017, 2:30 p.m....developed methods for selecting and amplifying RNA sequences that could bind very specifically to target molecules... -
Antibiotic Resistance: An Old Solution but a New Problem
TypeBlog PostPublishedNov. 10, 2016, 3:30 p.m....also arise from random mutations in the genetic sequence that happen to be beneficial, and persist as a... -
Antibodies 101: Producing Recombinant Antibodies
TypeBlog PostPublishedAug. 13, 2024, 1:15 p.m.... can vary depending on the specific amino acid sequence (Maity et al., 2015). If your antibody is not ... -
Viral Vectors 101: Producing Your rAAV
TypeBlog PostPublishedJuly 16, 2024, 1:15 p.m....virus serotype 2-derived inverted terminal repeat sequences. Human Gene Therapy Methods, 23(1), Article 1.... -
Tips for a 1st time AAV user (by a Rookie AAV user)
TypeBlog PostPublishedOct. 23, 2018, 11:49 a.m....virus serotype 2-derived inverted terminal repeat sequences. Human gene therapy methods, 23 1, 18-28. PubMed... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog PostPublishedJuly 27, 2023, 1:15 p.m....ssRNA strands (which can have a different genomic sequence from one another) — by various mechanisms, only...PMC2258930 Hu, W.-S., & Temin, H. M. (1990). Genetic consequences of packaging two RNA genomes in one retroviral... -
Lentiviral Vector Uses and Overview
TypeBlog PostPublishedMay 19, 2016, 2:30 p.m....with a lentiviral vector containing an antisense sequence against the HIV-1 envelope. As of 2013, 65 patients... -
The Challenges of Cell Culture
TypeBlog PostPublishedMay 5, 2016, 2:30 p.m....testing, from animal doping to forensics, DNA sequencing to drug testing. You can find Nick on LinkedIn... -
Plasmids 101: Protein tags
TypeBlog PostPublishedDec. 11, 2014, 4:26 p.m.... An affinity tag, generally a relatively small sequence of amino acids, is basically a molecular leash... -
Plasmids 101: The Promoter Region – Let's Go!
TypeBlog PostPublishedApril 3, 2014, 8:05 p.m.... and found upstream of their target genes. The sequence of the promoter region controls the binding of... -
Troubleshooting and Optimizing a Western Blot
TypeBlog PostPublishedSept. 17, 2024, 1:15 p.m....Plasmid-based proteins: plasmid sequence not accurate Check plasmid sequence for frameshifts and stop codons... -
The Fluorescent Vegetables in Aptamer Soup
TypeBlog PostPublishedJan. 29, 2019, 2:27 p.m....processes including RNA movement by tagging RNA sequences with an aptamer and RNA transcription by placing...