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We narrowed to 694 results for: ust

Showing: 381 - 400 of 694 results

  1. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ... forward or reverse screen (CRISPR, drug, etc.), just with an added baseline component. Alternative screening...cells and then assay cells which survive as these must have acquired a favorable mutation. But if the cellular...

  3. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...Blue-white selection is a widely used method to do just that! (To read about other screening strategies,...are a few common vectors. Blue-white screening is just that – a screening process. It does not select only...

  5. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    Published
    Aug. 7, 2014, 1:58 p.m.
    ... Gordon Conferences). You don’t want to be so exhausted that you can’t get a lot from the presentations... plan to meet up at the next conference. Once is just a meeting, but having lunch twice turns a stranger...

  6. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...genetic modification (GM) and synthetic biology. The mistrust and anger directed at GM crops has burdened this...imagine any use for GM technology other than to sustain those techniques. We feel strongly that the scientific...

  9. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...interactions (yeast-two hybrid, Co-IP, BioID),  and that’s just to name a few. But what if you want to study RNA-protein...serum iron levels seen in HHCS patients. These are just three examples of how RaPID can be used to study...

  13. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...I love Addgene's new Antibody Guide and not just because I helped create it. But while antibodies, as...there are many other amazing affinity reagents we just didn't have the space to give proper credit to. ...

  14. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...molecules. With the direct approach, primary antibodies must be either sourced already conjugated or conjugated...different signaling molecules. Many labs find that a trusted stock of secondary antibodies saves time and money...

  15. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    Published
    Oct. 1, 2019, 1:21 p.m.
    ...thanks for looking me up on LinkedIn!” resulting in just a little more relationship. Pro tip: Once you are...networking--”If it feels creepy, don’t do it.” It’s just not a useful way to build or use a network. Total...

  16. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...targeting Cas enzymes Move over Cas9, CRISPR isn’t just for DNA anymore! Cas enzymes have been isolated ...traditional spCas9. If you are looking to modify RNA, Cas9 just won’t cut it – literally. Cas13 Cas13 is an RNA-guided...

  17. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Published
    July 25, 2017, 1:20 p.m.
    ...and, like the game of telephone, often the signal must be transmitted by intermediate messengers. Optical...Western blot. Phosphorylation of MEK1/2 peaks at just 5 minutes post-Raf1 inactivation, and then decreases...

  19. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    .... The benefits of lab management software aren’t just limited to large volume facilities; it can be useful...ensure that everything is grown successfully, we must track and manage all of these steps. Unfortunately...

  20. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...software such as Primer3 to help you design your custom primers. Remember, when designing a sequencing ...outlined above. Avoid primers that bind within or must read through repetitive regions (such as LTRs or...
Showing: 381 - 400 of 694 results