We narrowed to 707 results for: ust

... a genomic alteration at a specific location, it must be accessible by Cas9. Our previous blog post The...important for base editing techniques, where Cas9 must be directed to a very specific site. Specificity...

...tendency to go back and forth on email rather than just picking up the phone and having a discussion. By...LLP, auditing insurance, finance and high-tech industries. He has earned both an MBA in finance and a B.S...

...and many of the existing GCE tools are not very robust. When using GCE, each component of the experiment...turning GCE tools developed in the literature into robust and universal techniques that researchers can easily...

...presentation by Professor Guido Kroemer from the Institut Gustave Roussy. Professor Kroemer discussed the effects...26343581. PubMed Central PMCID: PMC4597191. 5. Eyquem, Justin, et al. "Targeting a CAR to the TRAC locus with...

...positive and negative cells. This can be achieved by adjusting your voltage using your single color controls ...preserve your sample, and you should then confirm or adjust the settings with experimental samples. ...

...light up in response to calcium, which is more than just the stuff that makes bones strong; it plays critical...newer lasers in the range of 1000–1080 nm. These industrial fiber lasers are becoming increasingly common...

... forward or reverse screen (CRISPR, drug, etc.), just with an added baseline component. Alternative screening...cells and then assay cells which survive as these must have acquired a favorable mutation. But if the cellular...

...Blue-white selection is a widely used method to do just that! (To read about other screening strategies,...are a few common vectors. Blue-white screening is just that – a screening process. It does not select only...

... and her colleagues in Feng Zhang’s lab describe just such a system, which they call CRISPR/Cas9 Synergistic...reports, “Here we have shown that the SAM system is robust, specific, and can facilitate genome-scale gain-of-function...

... Gordon Conferences). You don’t want to be so exhausted that you can’t get a lot from the presentations... plan to meet up at the next conference. Once is just a meeting, but having lunch twice turns a stranger...

...genetic modification (GM) and synthetic biology. The mistrust and anger directed at GM crops has burdened this...imagine any use for GM technology other than to sustain those techniques. We feel strongly that the scientific...

...interactions (yeast-two hybrid, Co-IP, BioID),  and that’s just to name a few. But what if you want to study RNA-protein...serum iron levels seen in HHCS patients. These are just three examples of how RaPID can be used to study...

...DNA sequence and RNA expression - but what if you just want to change protein levels? Until recently, no... no such tool existed for rapid, customizable protein degradation. Enter TRIM-Away - an antibody-based...

...a scientist. I pretty quickly figured out that I just don’t have the patience to be a scientist,” says....  Another difficulty is that some articles are just harder to write. “There’s something about being ...

...I love Addgene's new Antibody Guide and not just because I helped create it. But while antibodies, as...there are many other amazing affinity reagents we just didn't have the space to give proper credit to. ...

...molecules. With the direct approach, primary antibodies must be either sourced already conjugated or conjugated...different signaling molecules. Many labs find that a trusted stock of secondary antibodies saves time and money...

...Exclusively Activated by Designer Drugs (DREADDs) (Armbruster et al., 2007).  We initially used a construct...disorders.   References and Resources References Armbruster BN, Li X, Pausch MH, Herlitze S, Roth BL (2007...

...communication is not just for neurons. Non-neuronal cells do this too. It's just that for non-neural cells...

...thanks for looking me up on LinkedIn!” resulting in just a little more relationship. Pro tip: Once you are...networking--”If it feels creepy, don’t do it.” It’s just not a useful way to build or use a network. Total...

...were covered in the workshop. These workshops illustrate the open and modular nature of the workshop content...that it is driven by academics, with support of industry partners, not the other way around. Sometimes,...