We narrowed to 855 results for: NTS

...on during the symposium was “How many graduate students/post-docs?” You can find some of the more detailed...even than the $21/h [on the low end] that grad students are paid before fringe benefits); what they don...receive as postdocs is not transferrable to other environments. Slide from Paula Stephan’s Talk (All slides...standard option for scientists by requiring graduate students to pay for part of their tuition. Having trainees...postdoc salaries be paid on fellowships instead of grants such that PIs no longer save grant money by paying... still be there without restructuring. Positive Points for Trainees - Taking Steps to Achieve a Successful... all traits you are likely to find in graduate students and postdocs. Trainees however, need to be made...

...that find events stressful. Video chatting makes it pretty easy to make 30 minute appointments with people...academia is run by grant funding. Learning to write grants quickly, efficiently and effectively is a valuable...valuable skill. There are many different kinds of grants that you can apply for. If you are in a lab, your...practicing and getting familiar with the process. Bonus points for being able to just look at a paragraph and ...Here are some sites that have compiled lists of grants: Science: Where to Search for Funding The Ohio...For Your Research University of Florida: Training Grants and Other Funding Opportunities L’oreal USA For...that you've been working on, let us know in the comments below! Additional resources on the Addgene blog...

...the pandemic, it’s hard to get these reagents. However, when reagents have been made available through a...because they provide rapid access to validated reagents, especially during a time when these materials...Everyone. Case study 1: Cell lines Many of the reagents used to investigate SARS-CoV-2 were developed ...endeavor. Case study 2: Plasmids Another example of reagents that have broad applications but have proven useful...plasmids and resources! Case study 3: Mouse strains Reagents developed during the SARS-CoV pandemic more than...from the scientific community. Deposits of new reagents, policies from journals and granting agencies ...for storing, validating, and distributing such reagents are all ways in which the scientific community...

...muscle contractions) are produced by orchestrated movements of networks of proteins consuming molecular fuel...undergoing local conformational changes. These tiny movements are then amplified tens or hundreds of thousands...decades, and they were known to be resistant to detergents and degradation under a variety of conditions...see figure to the right), we identified R-body mutants that extend at both lower and higher pH. We initially...initially used this screen to find R body mutants that couldn’t extend at a normal pH and later realized...F. R. Organization and expression of genetic determinants for synthesis and assembly of type 51 R bodies...

...large library of iGluSnFR3 mutants, identifying the two  best-performing variants as iGluSnFR4f (fast deactivation...iGluSnFR4f is well-suited for imaging rapid synaptic events such as in the somatosensory cortex, while iGluSnFR4s...display, they recommend the NGR vectors for most experiments. These new tools promise to be a popular choice...line creation a breeze. Modular by design: Key elements (promoter, tag, polyA site) are flanked by unique...insertion through targeted CRISPR homing of retroelements." The Abudayyeh-Gootenberg Lab developed this...based on several new site-specific retrotransposon elements they identified and characterized (Fell et al....untranslated regions (UTRs) outside of the homology elements allowed for scarless insertion — no UTR sequence...

...of the work, such as physically carrying out experiments like PCR and running gels, but in research you...in Ghana. My role involved teaching the nursing students and community health workers some basic biology...spreadsheets. It was great to be in the lab doing experiments  without the constant fear of failure because...company carries out clinical trials of drugs and treatments for external companies. It was really exciting... particular situation, communication between departments was sometimes difficult and the solution attempted... companies which are reliant on contracts with clients is that, when those contracts fall through or are...pancreatic cancer where one drug does not work for all patients, leading to very high mortality. I really loved...

...that stability of the RNA used in CRISPR-Cas9 experiments – the “gRNA” – suffers from the same issues as...nuclease-resistant, resulting in less off-target events. While both have similar advantages, bridged nucleic... Editing). This is particularly important in experiments in which viability is being compromised by introduction...CRISPR-Cas9 system can initiate targeted editing events. Both technologies utilize chemical modifications...gRNAs? Dye labeling the RNA in your targeting experiments allows you to visualize transfection efficiency...efficiency and even track localization in cellular experiments. There are many suitable dyes to choose from ...-703 (2020). https://pubs.acs.org/doi/10.1021/acscentsci.9b01093 More resources on addgene.org  View ...

...small errors that significantly impacts your experiments. What do you do when you’re in the lab and you're... steps like “check drawers for light-sensitive reagents” or “re-sterilize now.” Have a physical copy of...Have a physical copy on hand as you run your experiments. Try drawing out a protocol as you read it, or...an investment in accuracy.  Bench setup Small adjustments in the physical organization of your workspace...you track samples as you add them. Moving your reagents from your left side to your right as you use them...are endless.    Fig. 2: Blugene moves reagents from left to right as they use them.    Prevent...focus, which was extremely effective. Others ran experiments at odd hours, used more secluded workspaces, ...

... model. AAV plasmids contain several important elements, but among the most important are the inverted...inverted terminal repeats (ITRs). ITRs are the elements that make an AAV plasmid an AAV plasmid.  Within the... and initiate replication. ITRs are cis-acting elements, which means that they are in the same DNA molecule...types  Since ITRs are one of the most important elements of AAV and rAAV vectors, modifications can affect...prevent unwanted mutations?  During propagation, mutants rapidly replicate in a growth culture and result... sequencing is also helpful in quantifying ITR mutants since analysis of individual reads allows for identification...the Addgene blog Viral Vectors 101: Viral Vector Elements Viral Vectors 101: Pseudotyping Viral Vectors ...

...consumer-grade components. In this field, often referred to as 'frugal science', existing components are adapted...opportunities) to modify the device for individual experiments. Manufacturers rarely provide the construction... by the manufacturers and enables customized experiments. Projects such as the OpenFlexure Microscope ...   Taking this all together, the essential ingredients of open-source microscopy are as follows: Detailed...around the use of commercially available tools and components. Rapid prototyping techniques such as 3D printing...can literally put their work on the shoulders of giants. The still relatively young movement of sharing...similar goal. The sharing of modules, ideas, and blueprints has the potential not only to accelerate scientific...