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We narrowed to 803 results for: TIM

Showing: 401 - 420 of 803 results

  1. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ...University of Cambridge and knew it was time to leave. I really enjoyed my time in the lab, my colleagues and what...choices were mostly by chance. Luckily, around the time I decided to look for work outside of academia, ...

  2. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ... job. EAs shouldn't be viewed as an unproductive time spent away from the bench, but as an opportunity... conferences and seminars, as this is a valuable time to network Attend informational interviews to ...required qualifications and skill sets Taking the time to identify and research 2 – 3 potential professions...

  3. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ... DAP array, short for “drive-and-process.” To optimize DAP use, they developed a generalizable method...sequences must be avoided, different tRNAs with optimized 5’ leader sequences can be used together in DAP... Reporters to Monitor and Enrich Editing in Real Time Prime Editing: Adding Precision and Flexibility ...

  4. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ...iGluSnFR and SF-iGluSnFR have made it clear that it’s time to update our iGluSnFR post! Here, we look at the...generations of iGluSnFR had limitations: they were not optimal for experiments requiring high signal to noise ...Tools References Marvin, Jonathan S., et al. “An optimized fluorescent probe for visualizing glutamate neurotransmission...

  5. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...Group (QCRG) to do just this.  In under a month’s time, we’ve published an article on BioRxiv describing...!) Attacking coronavirus, one FedEx package at a time Since the publication of our paper, our group has...fulfillment manually in a shared spreadsheet and it takes time away from our research. For all these reasons, the...

  7. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ... was to be revisited at meetings when sufficient time, additional public fora for data review, and more...consensus guidelines, but there was never a specific timeline for this plan—or any action to follow it. And ... 2018 saga of “what’s happening in genetics this time?” She lives with her partner (in business and life...

  8. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...it required only about 15 minutes of my hands on time and a total of about 3 hours. It was robust, capable...to learn something new without spending a lot of time developing and making space for the techniques ourselves... ourselves. As you probably have limited time and space in your own lab, you should give similar workshops...

  9. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    Published
    Sept. 19, 2017, 1:20 p.m.
    ...transparent, so it’s easy to watch development in real time.  In both cases, dL5** was tagged with mCerulean3...They found that KillerRed required longer exposure times (5 minutes vs 10 seconds) to achieve the same level...experienced minor bleaching after longer exposure times. Additionally, KillerRed requires mitochondrial ...

  10. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    Published
    April 19, 2017, 3:25 p.m.
    ...as the relatively low cost and quick turn-around time of Sanger sequencing have historically matched the...The cost of these normalization steps, measured in time and consumables, can be daunting for large numbers... have worked with Addgene to develop a highly optimized bioinformatics pipeline that will return high-...

  11. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...easy to work with Grows extremely fast (doubling time of ~ 2 hours) Amenable to genetic manipulation ... K. phaffii’s genome has been sequenced for some time, clearing the greatest hurdle for genome engineering...cerevisiae and S. pombe - aren’t going anywhere anytime soon, and for good reason! However, there are other...

  12. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ... gRNA library, and we thought it would be a good time to talk about the amplification and verification...in the library. Bacterial growth must also be optimized to maintain library diversity. If the various ...seemingly straightforward – you need to count how many times each of the library components is found in the read...

  13. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...emails, chats, pictures, and apps. Throughout my time as a graduate student, I have relied more and more...requires cell culture and include a counter, lab timer, dilution calculator, and a couple of other nice... Lastly, the Tm calculator helps you set up an optimal PCR depending on the primer sequence and the NEB...

  14. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ...microbiology class my sophomore year and it was the first time that I was in a lab class where we had an assignment... me away….  I have to come here, I need to spend time here, this is an incredible place.” Doench has spent...CRISPR, just the results are so much cleaner. The time frame from doing your primary screen to knowing ...

  15. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...appropriate ligand to activate the G proteins. It is estimated that there are approximately 950 different human...signaling cascades as a response to the extracellular stimuli. Ligand dissociation from the GPCR allows for binding...learn about GPCR signaling, researchers in the meantime have developed GPCRs for chemogenetic studies ...

  16. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...tend to ask similar questions, thus devoting some time to writing a solid personal statement will pay dividends...with your personal statement, while you make the ultimate case: why you? 7. Strength in numbers Encourage...clinch every opportunity for themselves. This is ultimately the wrong approach. It is very likely you know...

  17. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    Published
    Sept. 15, 2020, 1:15 p.m.
    ... negative selection in plasmid cloning.  But oftentimes, a selection strategy is not enough on its own...sequencing primers in our Molecular Biology Reference. Sometimes common  primers can't provide all of the information...plasmid. Please note that NGS verification is more time-intensive than Sanger sequencing or the other techniques...

  18. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    Published
    Dec. 6, 2018, 1:34 p.m.
    ...as several days. In 2015, the laboratory of long-time Addgene depositor Alice Ting used yeast display-...temperature range than BioID, which only functions optimally at 37℃. The increased efficiency of TurboID and...when they surface. But detection of the virus costs time and money, and viruses at low concentrations often...

  19. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ... where image processing algorithms for analyzing time-lapse images, quantitatively evaluating antibody...download the software for use, or they can modify and optimize the code, find and fix security-critical bugs,...the world don't have to start from scratch every time to pursue their scientific curiosity but can literally...

  20. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ...graduate student using viral vectors for the first time and learning how to purify AAV.  Second, you’ll ... team, working together to share information. Sometimes it is most helpful to use a lot of jargon and ...and have a technical, detailed approach. Other times, it is more helpful to use plain language and focus...
Showing: 401 - 420 of 803 results