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...how they’ll make it easier for you to find the plasmids you need. We'll be monitoring and improving the...this blog post for tips on using BLAST to verify plasmids. Analyzer guide A quick guide to all of ...browse the repository and quickly identify the plasmids and features you need for your next experiment...

... targeted mutagenesis experiments? Find EvolvR plasmids at Addgene! References Gaj, Thomas, and Pablo...mutagenesis to generate specific mutations in your plasmids Use REPLACER Mutagenesis to mutate your plasmid...Browse our CRISPR guide pages Find bacterial CRISPR plasmids ...

...that Addgene’s mission statement extends beyond plasmids, AAVs, and antibodies to our content, website,...mission is to enable that process. Our repository of plasmids, AAVs, antibodies, and data allows scientists ...Resources on the Addgene Blog Science careers Plasmids CRISPR Viral vectors Fluorescent proteins  ...

...Chudakov et al, 2007). We have developed a variety of plasmids that can be used in the OSER assay including CytERM-mKOkappa...documenting it in some other way. Besides the data, new plasmids and/or cells or organisms that are engineered ...Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular Localization Browse the entire...

...localized fusion proteins and do not require dedicated plasmids or constructs. It should be noted that the photostability...lightbulb or exchanging excitation filters). This avoids substantial changes in excitation power and will...Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular Localization Browse the entire...

... your own RANbodies? Great! All of the RANbody plasmids from Yamagata et al can be found here. Can’t find...consider making you own DNA ladders with these plasmids from Penn State Need help picking the right fluorescence...Resources on Addgene.org More nanobody expression plasmids can be found here Watch this video to learn more...

...Fluorescent protein plasmids Use FRET to study protein-protein interactions Deposit plasmids with Addgene! ...problematic because they require radiolabeled amino acids. The use of radioactive materials is highly regulated...

...Addgene.org Visit our cancer research plasmids and resources page  Find plasmids for your research Learn about...They deliver compounds like proteins and nucleic acids from cancer cells to the microenvironment. This ...

... that is something to recognize! Distributing plasmids, viral service, and more For the first time ...distribution to ensure scientists everywhere can access plasmids despite the evolving export/import rules. Our ...Addgene Resources on the Addgene website Find plasmids for your research  Learn about our viral service...

...antibodies, such as inclusion of non-canonical amino acids or peptide tags. 5) Strong protein-protein interactions...ConjugatesAntibodies 101: The Basics of Western BlottingPlasmids 101: Biotinylation Resources on Addgene.org...Addgene.org Unconjugated antibodies at AddgeneAntibody plasmids at Addgene...

...stringent wash conditions.   Find polyhistidine tag plasmids here! Where should I place the tag and how many...polyhistidine tag is a string of histidine amino acids on the N- or C- terminus of a protein. Neither the...from Addgene More Resources on the Addgene blog Plasmids 101: Protein Tags Antibodies 101: ChIP Antibodies...

...create it. But while antibodies, as the OG (as the kids say) affinity reagents deserved to be celebrated...of targets, including proteins, peptides, amino acids, drugs, metal ions, and cells. They can be used ...Resources Additional resources on the Addgene blog Plasmids 101: Aptamer FluorophoresAntibodies 101: scFVs...

.... Cells are cultured with isotope-labeled amino acids for several hours to several days, followed by a... a short period of growth with unlabeled amino acids. This washout period removes radio-labeled short-... Resources on Addgene.org Fluorescent Protein Plasmids and Resources Fluorescent Protein Guide: Biosensors...

...and ligation steps to build barcoded lentiviral plasmids containing one or more gRNAs. As each ligation...ligation products are a diverse pool of lentiviral plasmids. Multiple rounds of digestion and ligation result...Background with Our CRISPR Guide Pages Find CRISPR Plasmids Find More Tools for Combinatorial Engineering ...

... GFP marker (CatCH-GFP). The lab deposited AAV plasmids containing the active promoters at Addgene. Performance...of the retina.  Find the AAV synthetic promoter plasmids! Comparing synthetic promoters performance in ...blog posts Resources at Addgene.org Find AAV plasmids Find ready-to-use AAV viral preps Browse all viral...

...types they tested. Interested in using Nanoblades? Plasmids are available from Addgene. Genome editing primary...to DNA transfection of gRNA and Cas9 expression plasmids. These results are in line with previous research...out our comprehensive CRISPR guide Find CRISPR plasmids and resources Learn about viral production ...

... using an engineered CRISPR-Cas system. Nucleic Acids Research 41:7429–7437 . https://doi.org/10.1093/...Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, Davidsohn N, Housden BE, Perrimon N, Weiss R, Aach J, Collins...Addene.org Read Addgene's CRISPR Guide Find CRISPR plasmids for your research ...

...from researchers, and the vast majority of our plasmids and pooled libraries were originally described...links to later papers that successfully used said plasmids. We provide these references as our way of saying...Asked Questions Pages Learn What to Do with Your Plasmids Once You Recieve Them Get Help with the Addgene...

... genes are supplied on two different packaging plasmids: the pHelper plasmid containing Ad E2A, E4, and...carrying your gene of interest, and the two helper plasmids, commonly called “triple plasmid transfection”...genome-containing (full) and non-genome-containing (empty) capsids, thus leading to an overestimate of transducible...

... Visit Our Stem Cell Page Find Plasmids for Reprogramming Find Plasmids for Differentiation ...to a Nobel Prize for Dr. Yamanaka in 2012. Find plasmids for generating iPSCs iPSCs and ESCs closely resemble...