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We narrowed to 838 results for: IGH@

Showing: 801 - 838 of 838 results
  1. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ... and human embryonic stem cells, they found very high recombination percentages (78-79% after two rounds...
  2. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...business consulting, mathematics, and more. That’s right, we have non-traditional scientists working in our...
  3. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ... jGCaMP7c variant in AAV1. This variant exhibits high contrast between its resting state and its calcium-bound...
  4. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ... Career Scientists, including their "Making the Right Moves: A Practical Guide to Scientifıc Management...
  5. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...transfection into mammalian cells is fairly straightforward and the resultant cells can either express ...
  6. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ... an antibody labeled with Fc, Fab, heavy chain, light chain, constant region, and variable region. ...
  7. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...neuroscience with ever more efficient tools for light mediated neuronal control. The advantages of optogenetics...
  8. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...makes lentiCRISPR v2 great? It produces a ~10 fold higher titer virus, making it ideal for CRISPR pooled ...
  9. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...NHEJ is active throughout the cell cycle and has a higher capacity for repair, as there is no requirement...
  10. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...needs. The most often-cited reasons researchers might not post a preprint are reluctance to share their...
  11. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...limitation of using loxP sites is the inability to tightly control which loxP sites recombine if more than...
  12. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...The coregulator ‘core’ complex is composed of a tightly-bound invariant group of proteins, whereas the ...
  13. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...for a lay readership, and working with middle and high school students. One of these organizations was ...
  14. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...requestors use the plasmid in their own labs. We highly encourage depositors to provide plasmid maps whenever...
  15. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...vectors all have a genomic capacity that is much higher than other viral vectors (tens of kilobases; compared...
  16. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...constructs, ADAR2DD(E488Q/T375G) (REPAIRv2) had the highest on-target efficiency and the lowest amount of off-target...
  17. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...bacteria deploy proteosomes to degrade what the cell might consider junk protein. Though there are a number...
  18. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...hosts. Also, self-transmissible plasmids may have high copy numbers and reduced metabolic load (Meyer 2009...
  19. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...externally. Fruit flies are also relatively straightforward to manipulate genetically. Scientists can make...
  20. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...transduce multiple tissues. So how do you choose the right serotype? Keep asking questions!  Table 1: AAV Serotypes...
Showing: 801 - 838 of 838 results