We narrowed to 838 results for: IGH@
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TypeBlog PostPublishedAug. 25, 2015, 2:30 p.m....Then ask for their honest opinions -- this part might hurt, but if you pitch to enough people, you will...
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Another Pathway into Cells: iTOP
TypeBlog PostPublishedJune 23, 2015, 8:37 p.m.... and human embryonic stem cells, they found very high recombination percentages (78-79% after two rounds... -
Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]
TypeBlog PostPublishedApril 7, 2015, 4:21 p.m....Watson-Crick base pairing, allowing for rational, straightforward design. Since the technique relies on base ... -
Mentoring for Scientists: I Have a Mentor, What Now?
TypeBlog PostPublishedMarch 6, 2014, 7:12 p.m....knowledge to impart. An undergraduate can mentor a high school student, a graduate student can mentor an... -
Cultivating Community Science at BosLab
TypeBlog PostPublishedNov. 2, 2017, 1:35 p.m....business consulting, mathematics, and more. That’s right, we have non-traditional scientists working in our... -
"Build Your Plasmid": The Game - Play to Teach
TypeBlog PostPublishedJuly 17, 2018, 12:23 p.m....and, depending upon who your challenger was, you might have to drastically alter your strategy. I realized... -
Celebrate National Book Month with Addgene
TypeBlog PostPublishedOct. 3, 2019, 12:47 p.m....back actual magic (which had been lost for an age) fight in the Napoleonic Wars and against something even... -
New and Upcoming Viral Vectors - June 2019
TypeBlog PostPublishedJune 18, 2019, 1:15 p.m.... jGCaMP7c variant in AAV1. This variant exhibits high contrast between its resting state and its calcium-bound... -
Advice for Scientists Starting a Lab
TypeBlog PostPublishedApril 29, 2014, 1:43 p.m.... Career Scientists, including their "Making the Right Moves: A Practical Guide to Scientifıc Management... -
Plasmids 101: Mammalian Vectors
TypeBlog PostPublishedMarch 25, 2014, 3:15 p.m....transfection into mammalian cells is fairly straightforward and the resultant cells can either express ... -
Antibodies 101: Isotypes
TypeBlog PostPublishedOct. 19, 2021, 1:15 p.m.... an antibody labeled with Fc, Fab, heavy chain, light chain, constant region, and variable region. ... -
New and Upcoming Viral Vectors - May 2020
TypeBlog PostPublishedMay 28, 2020, 1:15 p.m....neuroscience with ever more efficient tools for light mediated neuronal control. The advantages of optogenetics... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog PostPublishedJan. 8, 2019, 1:54 p.m....makes lentiCRISPR v2 great? It produces a ~10 fold higher titer virus, making it ideal for CRISPR pooled ... -
CRISPR 101: Non-Homologous End Joining
TypeBlog PostPublishedApril 16, 2015, 3:45 p.m....NHEJ is active throughout the cell cycle and has a higher capacity for repair, as there is no requirement... -
Kiran Musunuru on the Newest TALEN Genome-Editing System
TypeBlog PostPublishedDec. 10, 2013, 3:13 p.m....In many cases, CRISPR may be more efficient. You might often start with CRISPR/Cas9, but TALENs do have... -
Transferable Skills Guide: Public Speaking
TypeBlog PostPublishedApril 20, 2023, 4 p.m....is important to know that the student presenting right before me had been assigned a structure paper with... -
Summer SciComm: Preprints
TypeBlog PostPublishedAug. 16, 2022, 1:15 p.m....needs. The most often-cited reasons researchers might not post a preprint are reluctance to share their... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog PostPublishedApril 22, 2015, 2:06 p.m....actually were not many hurdles. It was pretty straightforward surprisingly. So I think a nice thing about... -
Plasmids 101: Cre-lox
TypeBlog PostPublishedJan. 13, 2015, 3:47 p.m....limitation of using loxP sites is the inability to tightly control which loxP sites recombine if more than... -
Working with Nuclear Receptors
TypeBlog PostPublishedJuly 15, 2014, 4:03 p.m....The coregulator ‘core’ complex is composed of a tightly-bound invariant group of proteins, whereas the ... -
Antibodies 101: Stripping and Reprobing Western Blots
TypeBlog PostPublishedAug. 1, 2024, 1:15 p.m....western blot as normal! Stripping your membrane Alright, so you’ve dried your membrane and returned it ... -
Science communication: 9 Strategies to Get Your Foot in the Door
TypeBlog PostPublishedSept. 27, 2017, 1:23 p.m....education. If you suspect that science communication might be for you, don’t wait. Start communicating now.... -
Engaging with science and society at pgEd
TypeBlog PostPublishedSept. 20, 2018, 12:17 p.m....for a lay readership, and working with middle and high school students. One of these organizations was ... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog PostPublishedMay 24, 2018, 1:20 p.m....species one is working with, the protocol will slightly differ. For example, Girardia sp. and Dugesia ... -
Mouse Modeling, Part 2: Breeding and Crossing Mice
TypeBlog PostPublishedAug. 6, 2019, 12:55 p.m....your desired genes. Remember Punnett squares from high school biology? They will come in handy here! Crossing... -
Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog PostPublishedJuly 23, 2015, 2:30 p.m....supplementation with grant funds, money is always tight. Of course, unlike your traditional biology lab,... -
Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)
TypeBlog PostPublishedJune 5, 2014, 3:32 p.m.... job appealed to us. However, since the lab was highly focused on protecting intellectual property, it... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog PostPublishedFeb. 23, 2016, 3:30 p.m....Xiuhua Meng, Xiaolei Wei, and Ling Lu. (2016). “Highly efficient CRISPR mutagenesis by microhomology-mediated... -
Anatomy of a Plasmid Page at Addgene
TypeBlog PostPublishedFeb. 4, 2016, 3:30 p.m....requestors use the plasmid in their own labs. We highly encourage depositors to provide plasmid maps whenever... -
Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments
TypeBlog PostPublishedSept. 23, 2014, 3:05 p.m....some classic protocols on Protocol Online that you might find useful for your research. LabGuru Website: ... -
An Introduction to Adenovirus
TypeBlog PostPublishedOct. 3, 2023, 1:15 p.m....vectors all have a genomic capacity that is much higher than other viral vectors (tens of kilobases; compared... -
CRISPR 101: RNA Editing with Cas13
TypeBlog PostPublishedJuly 31, 2020, 12:30 p.m....constructs, ADAR2DD(E488Q/T375G) (REPAIRv2) had the highest on-target efficiency and the lowest amount of off-target... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog PostPublishedOct. 22, 2015, 2:30 p.m....with one or more of these parameters can lead to a high number of false negatives in Y2H screens. Testing... -
Plasmids 101: Protein tags
TypeBlog PostPublishedDec. 11, 2014, 4:26 p.m....bacteria deploy proteosomes to degrade what the cell might consider junk protein. Though there are a number... -
Plasmids 101: Broad Host Range Plasmids
TypeBlog PostPublishedApril 11, 2024, 1:15 p.m....hosts. Also, self-transmissible plasmids may have high copy numbers and reduced metabolic load (Meyer 2009... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog PostPublishedDec. 14, 2017, 2:08 p.m....amplification in the host cell. This enables relatively high and long-term expression of the reprogramming factors... -
Five Popular Model Organisms
TypeBlog PostPublishedApril 11, 2019, 12:39 p.m....externally. Fruit flies are also relatively straightforward to manipulate genetically. Scientists can make... -
Important Considerations When Using AAVs
TypeBlog PostPublishedJune 13, 2017, 2:30 p.m....transduce multiple tissues. So how do you choose the right serotype? Keep asking questions! Table 1: AAV Serotypes...