We narrowed to 614 results for: CaS

...Addgene relied on Sanger sequencing for QC. We occasionally still use Sanger sequencing to confirm an insert...are highly repetitive, such as those containing cassettes of the same gene multiple times.  Bioinformatics...

...hydrogen bonds holding the DNA together, it recruits helicases to continue unwinding the strands. This creates...present in the chromosomal DNA ensure that the helicases are released from the DNA once the replication...

...the first time. They can then plan accordingly in case you have questions or need a little assistance at...questions later – maybe even the next day. In the latter case, you can say “I usually need time to gather my thoughts...

...copy number is high (del Solar et al., 1998).  In cases of compatible plasmids, different negative regulation...replication or partitioning machinery (Novick, 1987). In cases where one plasmid outcompetes the other, usually...

...relies on dimerization for activation, only in this case ligand binding would bring the relevant ends together...RNA-based scaffolds to contain and stabilize Broccoli cassettes, thereby extending their use into mammalian cells...

...other tools in conjunction with the ELN, in some cases the integrations provide a direct boost to reproducibility...restrictions based on permissions, however, in some cases it would be possible to grant those wanting to understand...

...research being published on our own topic. Only on occasion do we then have time to read about research from...video abstracts in science journals: An overview and case study." Journal of Librarianship and Scholarly Communication...

...initially isolated. Regrettably this is not usually the case if you obtain a cell line from a colleague. Time... been directly isolated from patients. In these cases, a test known as Short Tandem Repeat (STR) profiling...

...density within a reasonable time-frame. For these cases, some strains carry an additional measure of control... plasmid contains a chloramphenicol resistance cassette for positive selection and a p15A origin of replication...

...approach is reliable, but it’s overkill in many cases. Often, you may not need to know the exact quantity...Although this assumption is likely true in many cases, you should validate it for your particular setup...

...is that my suggestions can help you make the best case for your candidacy. A major reason that a lot of...encouraged me to publish this manual and earn some cash for my expensive habits of running shoes and single...

...approaches to record keeping. Top priorities in most cases include IP protection, publications and funding....site licenses might be available as well. In most cases this applies to universities. How are you currently...

...any additional work you might need to do. In most cases, I would recommend the second option for the experiment... gave me time to listen to some of my favorite podcasts. JUST GET STARTED - One Harvard Business Review...

...to the occasion Don’t just plan to reuse a prepared talk unless it perfectly fits the occasion.  I speak...

... will increase the power of the results. In this case, mouse samples from positive (+), negative (-) and...antibodies will be highly specific, but in some cases they may cross-react and bind to more than their...

... film or with specific imaging devices. In most cases, the primary antibody, which binds the protein of...inconsistent levels of the loading control. In these cases, the western should be repeated.  Finally, while...

...-fidelity polymerase to ensure accuracy. In this case, you would want a low-fidelity polymerase, like ...different chimeras might function better. In that case, gene shuffling might be a good option. In gene ...

...designations all refer to the same test. On some occasions, there is an experimental group being compared... being between control vs. experimental. In this case, save yourself from family-wise error and just perform...

...to knock-down expression of your target or CRISPR/Cas9 to create mutations that essentially eliminate expression...gene for your protein has been mutated using CRISPR/Cas9. You check the mutation and it causes a large deletion...

...gene of interest with an antibiotic resistance cassette.   Substrate DNA Design and Generation Whether...can be used to screen for positive clones in most cases, and restriction enzyme digest can be used to screen...