We narrowed to 872 results for: FER

...You’ll want to pick enzymes that will give you different sized bands depending on whether the plasmid contains...using vector-specific primers, look for size differences. You can also use primers where one primer anneals...popular sequencing primers in our Molecular Biology Reference. Sometimes common  primers can't provide all of...Sanger sequencing Read the Molecular Biology Reference ... Jennifer Tsang...

...These cells are then transfected with several different constructs for the reporter to work and to make... visual read out allows for easy screening of different gRNAs by simply changing the gRNA used when transfecting...concept the Denham lab compared editing at three different genomic sites in the AAVS1 locus using GEmCherry2...GEmCherry2. In this experiment they saw a clear difference in mCherry expression using FACs sorting, confirming...mutant of interest, give these methods a try.  References Højland Knudsen C, Ásgrímsdóttir ES, Rahimi...

...commonly used in research laboratories to detect the different subclasses, but there are also secondary antibodies...secondary antibodies to detect multiple targets with different mAbs in the same experiment. The Trimmer Lab found...within the plasmid backbone to generate R-mAbs of different subclasses, including subclasses that were not... useful research materials and information. References and Resources Additional Resources on the Addgene...Addgene's recombinant antibody plasmid collection References Andrews NP, Boeckman JX, Manning CF, Nguyen JT...

...the answer isn't to work harder - it's to work differently.  Tools to Try  Checklists Found in surgical ...and your workflow can have a huge impact. Test different approaches, like whether it's better for you to... more than anything else I tried, made a huge difference in my ability to focus, especially on days when...what can be a rather vicious cycle.  Everybody’s Different   The truth is, there is no One True Way to Error-Free...the detail-driven daily grind of science.      References and Resources Images used Roberson Lab, UPenn...

...illustrates the utility these tools have in many different areas of biology as they grow. The top 15 plasmids...envelope protein enables this virus to infect many different cell types so it is useful in a wide range of ...lentiCRISPR v2 - This 3rd generation lentiviral transfer vector also from the Zhang lab is used to deliver... David Root lab is a 3rd generation lentiviral transfer plasmid for cloning and expressing new shRNA sequences... Jennifer Tsang...

...DNA. The other component of these systems, methyltransferases, methylate DNA at particular sequences to...restriction modification systems containing methyltransferases that add methyl groups to specific DNA sequences...contain 3 methylases that recognize and methylate different stretches of DNA: Dam methylase adds a methyl...plasmids with no methylation (there are no methyltransferases in the PCR reaction). The template plasmid...could overlap with a methylation site. The quick-reference table below lists 10 common enzymes that may be...

...to give you advice and guidance. Second, I will offer some advice on how to “make the ask” once you have...have a “posse” of advisors who can help us in different ways and in diverse areas. It is helpful to have...information in these searchable databases are usually offering their contact information so you can tap them ...someone you trust. A good coach can make a big difference, if you can find one. Create your own peer mentoring...with progress. Tell them how their advice made a difference, helped you make a change for the good or resulted...

...lots of fun building and clicking. References and Resources References Devaraj, N. K., & Finn, M. G. (2021...first time. But biology is complicated — so many different parts and species, with components quickly losing...proteins to any kind of small molecule probes with different functionalities, such as fluorescence or triggering...deposited at Addgene in multiple formats: for use in different organisms, targeted to particular compartments...

...protein expression journey! Resources and References References Nabet et al. The dTAG system for immediate...turn off expression for good, the Cre-Lox system offers inducible yet permanent gene excision. Essentially...days. There’s also a CRISPR for that! CRISPR interference (CRISPRi) targets a catalytically dead Cas9 ..., and expression is tunable by titration with different amounts of tetracycline. Tetracycline regulation...

...mammalian systems has been in question. Three different labs have recently published work that provides... gene activation  and base editing, whereas a different set of mutations (the “V3.1” version) works best...miniature Cas system that utilizes a Cas12f from a different species, Acidibacillus sulfuroxidans, AsCas12f1...They further develop a version of SEND for gene transfer with pseudotyped viral particles, expanding options... the Liu Lab found that mismatch repair (MMR) interfered with the desired resolution of prime editing ...

... put your experiments Nsync.   References and resources   References Dolan, E., Wang, Hou, Zhonggang,...while also highlighting their similarities and differences to Cas9. DNA targeting Cas enzymes Cas9 – The...Originally classified as Cpf1, Cas12’s primary difference from Cas9 is that it generates staggered breaks...(RNA knockdown, RNA editing, etc.). The main differences between Cas7-11 and Cas13 are 1). non-specific...

...billions—but when drugs fail, they cause human suffering and death. Doing research right has the potential... relevant? Research shows that there are sex differences in the therapeutic capacity of stem cells. Yet...male stem cells. Nor did they detect important differences in function between male and female cells. The...in basic research.  In addition to the studies referenced above, another excellent resource for research...population. To learn a little more about where differences in effects on the sexes come from, one clue or...

...plasmid) and any other important features that differentiate this plasmid from its predecessor. Important...production and storage, we grow a little extra and transfer 100 µl of glycerol stock to each of 2 96-well ...sequencing provider. Each sequencing service will have different requirements for volume and concentration, and...starts by determining what features you expect to differ between plasmids, and selecting or designing a ...Resources on the Addgene Website View our Plasmid Reference Pages Get Plasmid Backbone Information from Our...

...GBPb-GAL4 (DNA-binding domain); and a UAS-driven luciferase reporter construct. In the absence of GFP, no...create a complete transcription factor, activating luciferase transcription. Tang et al. coined this system... such that they activate gene expression from different promoters. Moving to an in vivo system, Tang et...eager to see how you use them in your research!  References 1.Tang, Jonathan C.Y., et al. “A nanobody-based...plasmids from this publication at Addgene. 3. Kirchhofer, A., et al. “Modulation of protein properties...

...difficult to study ncRNA, CRISPR systems offer a number of different options. By carefully considering your...without permanently altering the DNA, CRISPR interference (CRISPRi) and CRISPR activation (CRISPRa) are... you more about its function.  Resources and references Resources on addgene.org Addgene's CRISPR Guide... CRISPR enzymes for transcriptome engineering References Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna...

... the E1 gene from the packaging cell line is transferred into the AdV by recombination. It is therefore...for production of Gutless AdV can be found in reference 3. Production, amplification, and quality control...please see Addgene’s Adenoviral Guide webpage, and references 1, 2.*Pro-Tip*: once the correct recombinant ...specific for a viral DNA sequence. *Pro-Tip*: Ad5 reference material should be used as an internal control..., high-titer rAdV in your experiment. Enjoy! References 1. Luo, Jinyong, et al. "A protocol for rapid...

...easy — our tech transfer team will communicate with your organization’s technology transfer office or other... Send plasmid information to Addgene Material Transfer Agreement (MTA) approval  Send plasmids to Addgene...expression, lentiviral, retroviral, AAV, RNAi, luciferase, cre/lox, yeast expression, worm expression, ...samples to Addgene and contact your technology transfer office about the MTA. Pro Tip! Download a new...

...updated by Jason Nasse in July 2023.  References and Resources References Chen X, Choo H, Huang X-P, Yang X...lead to such responses as decreased heart rate (Redfern et al., 1999) or altered neuron excitability (Zhu...to consider that the same receptor can elicit different responses depending on what cell type it is expressed....sciencemag.org/content/364/6436/eaav5282.long Redfern CH, Coward P, Degtyarev MY, Lee EK, Kwa AT, Hennighausen...

...activation by dCas9-CBP and the SAM system differ in target preference. Sci Rep 9: . https://doi.org/10.1038...programmable guide RNA. Gene activation by dCas9, also referred to as CRISPRa, was initially published in 2013...method, dCas9 is fused to CBP, an histone acetyltransferase domain capable of rearranging chromatin structure...experiment. Good luck with your experiment! References Bikard D, Jiang W, Samai P, Hochschild A, Zhang...

... Clinical Ophthalmology Basel recently took a different approach to enhance specificity in retinal cells... synthetic promoters, the Roska lab used four different design strategies: Strategy #1 (ProA): Create... this library contained 226 AAVs, each with a different synthetic promoter driving expression of the same...was from a single class of cells  Of the four different promoter designs, promoters from strategy #4 had...ready-to-use AAV viral preps Browse all viral vectors References Juettner, J., Szabó, A., Gross-Scherf, B., Morikawa...