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We narrowed to 867 results for: FER

Showing: 401 - 420 of 867 results

  1. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ...These cells are then transfected with several different constructs for the reporter to work and to make... visual read out allows for easy screening of different gRNAs by simply changing the gRNA used when transfecting...concept the Denham lab compared editing at three different genomic sites in the AAVS1 locus using GEmCherry2...GEmCherry2. In this experiment they saw a clear difference in mCherry expression using FACs sorting, confirming...mutant of interest, give these methods a try.  References Højland Knudsen C, Ásgrímsdóttir ES, Rahimi...

  2. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    Published
    Feb. 4, 2014, 3:50 p.m.
    ...to give you advice and guidance. Second, I will offer some advice on how to “make the ask” once you have...have a “posse” of advisors who can help us in different ways and in diverse areas. It is helpful to have...information in these searchable databases are usually offering their contact information so you can tap them ...someone you trust. A good coach can make a big difference, if you can find one. Create your own peer mentoring...with progress. Tell them how their advice made a difference, helped you make a change for the good or resulted...

  3. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ...billions—but when drugs fail, they cause human suffering and death. Doing research right has the potential... relevant? Research shows that there are sex differences in the therapeutic capacity of stem cells. Yet...male stem cells. Nor did they detect important differences in function between male and female cells. The...in basic research.  In addition to the studies referenced above, another excellent resource for research...population. To learn a little more about where differences in effects on the sexes come from, one clue or...

  4. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    Published
    Feb. 16, 2023, 7:05 p.m.
    ...protein expression journey! Resources and References References Nabet et al. The dTAG system for immediate...turn off expression for good, the Cre-Lox system offers inducible yet permanent gene excision. Essentially...days. There’s also a CRISPR for that! CRISPR interference (CRISPRi) targets a catalytically dead Cas9 ..., and expression is tunable by titration with different amounts of tetracycline. Tetracycline regulation...

  5. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ... put your experiments Nsync.   References and resources   References Dolan, E., Wang, Hou, Zhonggang,...while also highlighting their similarities and differences to Cas9. DNA targeting Cas enzymes Cas9 – The...Originally classified as Cpf1, Cas12’s primary difference from Cas9 is that it generates staggered breaks...(RNA knockdown, RNA editing, etc.). The main differences between Cas7-11 and Cas13 are 1). non-specific...

  6. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...mammalian systems has been in question. Three different labs have recently published work that provides... gene activation  and base editing, whereas a different set of mutations (the “V3.1” version) works best...miniature Cas system that utilizes a Cas12f from a different species, Acidibacillus sulfuroxidans, AsCas12f1...They further develop a version of SEND for gene transfer with pseudotyped viral particles, expanding options... the Liu Lab found that mismatch repair (MMR) interfered with the desired resolution of prime editing ...

  7. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...plasmid) and any other important features that differentiate this plasmid from its predecessor. Important...production and storage, we grow a little extra and transfer 100 µl of glycerol stock to each of 2 96-well ...sequencing provider. Each sequencing service will have different requirements for volume and concentration, and...starts by determining what features you expect to differ between plasmids, and selecting or designing a ...Resources on the Addgene Website View our Plasmid Reference Pages Get Plasmid Backbone Information from Our...

  8. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    Published
    Nov. 24, 2015, 3:30 p.m.
    ...GBPb-GAL4 (DNA-binding domain); and a UAS-driven luciferase reporter construct. In the absence of GFP, no...create a complete transcription factor, activating luciferase transcription. Tang et al. coined this system... such that they activate gene expression from different promoters. Moving to an in vivo system, Tang et...eager to see how you use them in your research!  References 1.Tang, Jonathan C.Y., et al. “A nanobody-based...plasmids from this publication at Addgene. 3. Kirchhofer, A., et al. “Modulation of protein properties...

  9. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...updated by Jason Nasse in July 2023.  References and Resources References Chen X, Choo H, Huang X-P, Yang X...lead to such responses as decreased heart rate (Redfern et al., 1999) or altered neuron excitability (Zhu...to consider that the same receptor can elicit different responses depending on what cell type it is expressed....sciencemag.org/content/364/6436/eaav5282.long Redfern CH, Coward P, Degtyarev MY, Lee EK, Kwa AT, Hennighausen...

  10. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ... the E1 gene from the packaging cell line is transferred into the AdV by recombination. It is therefore...for production of Gutless AdV can be found in reference 3. Production, amplification, and quality control...please see Addgene’s Adenoviral Guide webpage, and references 1, 2.*Pro-Tip*: once the correct recombinant ...specific for a viral DNA sequence. *Pro-Tip*: Ad5 reference material should be used as an internal control..., high-titer rAdV in your experiment. Enjoy! References 1. Luo, Jinyong, et al. "A protocol for rapid...

  11. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    Published
    Aug. 6, 2019, 12:55 p.m.
    ...separately introduce each mutation independently into different mice, and then these strains should be crossed...When crossed to a mouse that is homozygous for a different mutation, the F1 offspring will be either hemizygous...include embryonic lethality, a short lifespan, or infertility and can arise due to copy number, expression ...transgene. These problems can be mitigated in different ways. Prior to engineering a transgenic strain...engineered mice. Best wishes on your experiment! References Becher, Burkhard, Ari Waisman, and Li-Fan Lu....

  12. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...difficult to study ncRNA, CRISPR systems offer a number of different options. By carefully considering your...without permanently altering the DNA, CRISPR interference (CRISPRi) and CRISPR activation (CRISPRa) are... you more about its function.  Resources and references Resources on addgene.org Addgene's CRISPR Guide... CRISPR enzymes for transcriptome engineering References Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna...

  13. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...easy — our tech transfer team will communicate with your organization’s technology transfer office or other... Send plasmid information to Addgene Material Transfer Agreement (MTA) approval  Send plasmids to Addgene...expression, lentiviral, retroviral, AAV, RNAi, luciferase, cre/lox, yeast expression, worm expression, ...samples to Addgene and contact your technology transfer office about the MTA. Pro Tip! Download a new...

  14. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...concentrating a viral stock. Different titering methods can reveal differences in physical versus functional...will be used in a couple of days, some researchers prefer to store freshly harvested virus at 4 °C instead...up-to-date tips for your virus experiments. References 1. Anthony, Simon J., et al. "A strategy to estimate... Yarmush. "Polybrene increases retrovirus gene transfer efficiency by enhancing receptor-independent virus...

  15. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ... 1-4 different gRNAs. Viral envelope proteins To alter the cell tropism of Nanoblades, different viral...embryos, and animals. Nanoblades can also carry different types of CRISPR cargo including RNPs for indel...can then fuse with the target cell membrane, and transfer their CRISPR cargo into the zygote. As a proof-of-principle...versatile and accessible genome editing tool.   References Mangeot, Philippe E., et al. "Genome editing ...

  16. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type
    Blog Post
    Published
    May 4, 2021, 1:15 p.m.
    ... we haven’t even imagined yet. References and Resources References Andrews NP, Boeckman JX, Manning ...consist of a mixture of antibodies recognizing many different epitopes. (Middle) Monoclonal antibodies produced...of Recombinant Antibodies Recombinant antibodies offer numerous advantages over polyclonal antibodies and..., Aldred SF, Bestagno M, Burrone O, Maynard J, Ferrara F, Trimmer JS, Görnemann J, Glanville J, Wolf P...

  17. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    Published
    Oct. 6, 2020, 1:15 p.m.
    ...activation by dCas9-CBP and the SAM system differ in target preference. Sci Rep 9: . https://doi.org/10.1038...programmable guide RNA. Gene activation by dCas9, also referred to as CRISPRa, was initially published in 2013...method, dCas9 is fused to CBP, an histone acetyltransferase domain capable of rearranging chromatin structure...experiment. Good luck with your experiment! References Bikard D, Jiang W, Samai P, Hochschild A, Zhang...

  18. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    Published
    March 12, 2020, 1:15 p.m.
    ... Clinical Ophthalmology Basel recently took a different approach to enhance specificity in retinal cells... synthetic promoters, the Roska lab used four different design strategies: Strategy #1 (ProA): Create... this library contained 226 AAVs, each with a different synthetic promoter driving expression of the same...was from a single class of cells  Of the four different promoter designs, promoters from strategy #4 had...ready-to-use AAV viral preps Browse all viral vectors References Juettner, J., Szabó, A., Gross-Scherf, B., Morikawa...

  19. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...these vectors is controlled by a different promoter resulting in different levels of expression from each...functional, stable proteins. Protein expression refers to the second step of this process: the synthesis...well-defined media and can be easily adapted to fermentation allowing for large-scale, stable production ...labelled or modified amino acids that are useful in different downstream applications. Cell-free expression ...

  20. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ... splitting transfected cells into 3 plates at different densities for selection). A 2% agarose solution...transfection into plates containing selection agent at different densities, then dispose of the plates that do ...freeze at least 2 ampules each of 4 clones with different levels of expression of our gene of interest. ...to rapidly isolate individual cell clones with different expression characteristics from a population of...cell clones picked using the agarose method do not differ grossly from those isolated using cloning rings...
Showing: 401 - 420 of 867 results