Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 845 results for: LEA

Showing: 401 - 420 of 845 results

  1. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ...stakeholders to learn more about their needs and any problems that they are trying to solve. After learning more ...Technologies, I work with our customers in the US to learn where the gaps are in their sequencing workflows...problem-solver. A lot of the time, the problem to solve is clear, but the solution is more complicated. You’ll need...

  2. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    Published
    Sept. 15, 2020, 1:15 p.m.
    ...experiments. As a common example, a selection will leave you with the colonies that contain your plasmid ...negative controls for your PCR.  Find the details and learn more about colony PCR in this blog post.   ...can confirm the sequence of the entire plasmid. Please note that NGS verification is more time-intensive...biology protocols and tips Resources on Addgene.org Learn more about Sanger sequencing Read the Molecular ...

  3. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...are some of the AAV and lentiviral preps we have released in the last few months: Lentiviral preps for ...coming soon! These vectors listed below should be released at Addgene in the next 6 months, pending quality... particular catalog item is available for order please email [email protected] with the vector name and...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...

  4. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ...fluorescent reporter assays to quantify targeted Cas9 cleavage, and repair through homology directed repair (...while cells containing the reporter gene that was cleaved by Cas9 and repaired via HDR using the supplied... seamlessly remove the integrated GFP reporter leaving you with just your desired genomic edit.  Find ...using GEmCherry2. In this experiment they saw a clear difference in mCherry expression using FACs sorting...

  5. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type
    Blog Post
    Published
    May 19, 2020, 1:15 p.m.
    ...collection in your experiments and published your data, please fill out this form and we’ll add your citation ...combinations of WT, codon optimized, untagged, cleavage sites (thrombin and TEV), and C-terminal tags ...downstream experiments and offer flexibility in tag, cleavage, codon usage, and expression system. Mammalian...Addgene blog Read all of our COVID-19 blog posts Learn about other COVID-19 plasmids available at Addgene...

  6. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...β-galactosidase mutation. An unsuccessful cloning reaction leaves the α-peptide intact, and therefore the cell will...artifact that disrupts the α-peptide DNA will also lead to a white colony. And also false negatives: These... fragment is inserted in-frame, read-through can lead to a functional β-galactosidase enzyme and a blue...Blog Read Our Post on Common Lab E. coli Strains Learn to Use Yeast Two Hybrid Systems Browse All of Our...

  7. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ....) Fab fragments are created through enzymatic cleavage of the variable region (the antibody region that...to the antigen) from existing antibodies. Each cleaved antibody will produce two ~50kD Fab fragments, ...fragment but created through engineering instead of cleavage. They are comprised of the variable heavy and ...Whatever affinity reagents you use, one thing is clear: it’s an exciting time to be a scientist looking...

  8. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    Published
    Aug. 5, 2021, 12:15 p.m.
    ...This biosensor changes fluorescence based on the release of glutamate from neurons and this new version ...than its predecessors. Currently, Aggarwal, and colleagues Filip Tomaska and Tim Hanson, are characterizing...iGluSnFR3 in vivo and have used it to detect glutamate release from hundreds of synapses at once. “This work has...Aggarwal.In addition to the biosensors, Aggarwal and colleagues are working on developing new surface display...

  9. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ... suited for particular applications. Read on to learn more about each reporter or check out table 1 for...nanobody that would make a useful RANbody, consider leaving a link to its sequence in the comments below. ...29440485. Additional Resources on the Addgene Blog Learn more about using the secondary nanobody toolbox ... plasmids can be found here Watch this video to learn more about nanobodies Additional Resources Looking...

  10. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ...week could expect to see their salary raised to at least a new legal minimum of $47,476 per year, under updates...universities with the FLSA in our online resource leading up to this date, as discussed in a previous Addgene...planning unionization at their institutions. One clear message that institutions have sent, with the lack...the Advocating for Science Symposium and Workshop Learn about a Variety of Science Careers Resources on...

  11. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...does it play in modern cloning, and why should you learn more about it? Read on to find out how ccdB can ...restriction enzyme-mediated disruption of ccdB, leading to positive identification of desired clones. ...most appropriate method for your own experiments. Please check out Addgene’s Choosing a Molecular Cloning...CcdB protein induces efficient ATP-dependent DNA cleavage by gyrase.” J Mol Biol. 1993 Dec 5;234(3):534-...

  12. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...Troubleshooting DNA Sequence Learn Additional Ways to Verify Your Plasmid Learn How Addgene Uses Barcodes.... All of our incoming plasmids are sequenced at least twice, and sometimes more depending on the number...read. You can expect to recover around 750 bp of clean sequence from a typical reaction, or about 250 amino...

  13. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    ...on the symposium as a whole and for registration please go to http://futureofresearch.org/boston/. The ... data and the Labor Market. On this topic it is clear that many important questions, areas for informed...biomedical_research_wgreport.pdf Resources at Addgene Learn More about Careers in Science in Our Career Posts...Meetings from Addgene Executive Director Joanne Kamens Learn how to be a successful manager with our Management...

  14. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ... "traditional" restriction enzymes in that they cleave outside of their recognition sequence, creating... BbsI), that are located on the outside of the cleavage site. As a result, these sites are eliminated ...a few advantages over other cloning methods. Exonuclease-based methods like Gibson assembly require 20...multiple DNA fragments to create TAL effector nucleases. These plasmids were designed to utilize the BsaI...

  15. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    Published
    Aug. 20, 2019, 12:31 p.m.
    ...spectrum. The experimental apparatus should be cleaned thoroughly between animals as olfactory stimuli...animal (ex: urine) can be distracting. If it is not cleaned, animals tested towards the end of an experiment...coordination to more complex situations such as learning and memory or emotional states, for example anxiety...Guide to Viral Vectors Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...

  16. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    Published
    May 24, 2018, 1:20 p.m.
    ...Arabidopsis in Education Learning Synthetic Biology with BioBuilder 3D Models for learning about CRISPR Resources... subsequently destroyed by Argonaute-catalyzed nuclease activity or their translation disrupted through...protocols and want to share pictures, feedback etc. please write to [email protected] and if you make...

  17. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    Published
    May 22, 2018, 1:38 p.m.
    ...Addgene Blog Learn about Synthetic Biology with BioBuilder Use Addgene Videos to Learn about Basic Lab...genes in other species, but these studies generally leave questions about gene function wide open. What if...Check Out Our Molecular Biology Reference Pages Learn Simple Lab Techniques in Our Protocol Videos ...

  18. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...adenovirus, and adeno-associated virus (AAV). The least risky of these four viruses is AAV – it is not known...propensity to integrate into proto-oncogenes, which can lead to cancer. These viruses need to be handled in a...thoroughly decontaminated after use, typically with bleach (plastic) or ethanol (other surfaces). For AAVs...and isopropyl alcohol, 0.5% peracetic acid, 10% bleach, or 1% iodine are useful. Some organizations or...

  19. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...unfortunate experience of witnessing…nothing. For some unclear reason, cells are not getting transduced. Here,...reducing the culture volume into which the virus is released, a more concentrated virus solution can be obtained...PMC330758. Additional Resources on the Addgene Blog Learn All about Adenovirus Associated Virus New to Virus...Virus? Check out Our Primer for Beginners! Learn All about Lentiviral Vectors Additional Resources on ...

  20. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...target DNA without cleavage until it's activated by light. Once activated, Cas9 cleavage can induce DSBs ...probes and editors. A probe array may consist of at least two sensor modules targeting a specific chromatin...complex binds to the degron sequence ultimately leading to the degradation of the AID-tagged protein. To...
Showing: 401 - 420 of 845 results