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We narrowed to 849 results for: LEA

Showing: 401 - 420 of 849 results

  1. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type
    Blog Post
    Published
    May 19, 2020, 1:15 p.m.
    ...collection in your experiments and published your data, please fill out this form and we’ll add your citation ...combinations of WT, codon optimized, untagged, cleavage sites (thrombin and TEV), and C-terminal tags ...downstream experiments and offer flexibility in tag, cleavage, codon usage, and expression system. Mammalian...Addgene blog Read all of our COVID-19 blog posts Learn about other COVID-19 plasmids available at Addgene...

  2. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ...ever like to write about a recent plasmid deposit please sign up here. Here's what you'll find in this...a continuation of that work, they have found at least two variants that display increased neuronal expression...microbial SSAPs (dCas9-SSAP) which insert genes in a cleavage free manner. The dCas9-SSAP system reports an ... less risky.   Fig. 3: Development of a cleavage-free dCas9-based gene editor using microbial SSAPs...

  3. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...ever like to write about a recent plasmid deposit please sign up here.  Here's what you'll find in this...of anti-FLAG-FB-GFP binding to FLAG-tag H2B for nuclear localization. (c) Applications of anti-FLAG-FB-...anti-FLAG-FB-GFP labeling various targets with nuclear (top, 4x-FLAG-mCherry-H2B), cytoskeletal (middle, 4xFLAG-mCherry-β-actin... editing, while gene editing can be done via a nuclease-active CasMINI. This small yet powerful system...

  4. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ...option, in aliquots no smaller than 10uL. You can leave them at room temperature when you’re working with...illustrious lab career, you will likely accidentally leave your antibody out on the bench overnight. Do not...this post, the conjugates, or signaling molecules! Please keep in mind that this is just a brief introduction...data, you’ll want to pick fluorophores with the least spectral overlap between them whenever possible....

  5. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    Published
    July 22, 2025, 1:15 p.m.
    ...Simplified chemical reaction catalyzed by luciferase, releasing light. Created with BioRender.com. Bioluminescent... luciferase reporters aren’t susceptible to photobleaching — they’ll keep emitting light as long as their...Sensitivity Higher Lower Background Lower Higher Photobleaching/phototoxicity Not susceptible Susceptible ...advantages of luminescent assays (low background, no photobleaching) but provides flexibility by pairing the luciferase...

  6. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...the nucleus through nuclear pores and thus do not require breakdown of the nuclear envelope during cell...there – most of which we would all like to steer clear of! However, a subset of viruses are commonly used...which ones could be helpful to you. Interested in learning more about a specific virus? We have you covered...

  7. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...β-galactosidase mutation. An unsuccessful cloning reaction leaves the α-peptide intact, and therefore the cell will...artifact that disrupts the α-peptide DNA will also lead to a white colony. And also false negatives: These... fragment is inserted in-frame, read-through can lead to a functional β-galactosidase enzyme and a blue...Blog Read Our Post on Common Lab E. coli Strains Learn to Use Yeast Two Hybrid Systems Browse All of Our...

  8. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ...week could expect to see their salary raised to at least a new legal minimum of $47,476 per year, under updates...universities with the FLSA in our online resource leading up to this date, as discussed in a previous Addgene...planning unionization at their institutions. One clear message that institutions have sent, with the lack...the Advocating for Science Symposium and Workshop Learn about a Variety of Science Careers Resources on...

  9. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...does it play in modern cloning, and why should you learn more about it? Read on to find out how ccdB can ...restriction enzyme-mediated disruption of ccdB, leading to positive identification of desired clones. ...most appropriate method for your own experiments. Please check out Addgene’s Choosing a Molecular Cloning...CcdB protein induces efficient ATP-dependent DNA cleavage by gyrase.” J Mol Biol. 1993 Dec 5;234(3):534-...

  10. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ... suited for particular applications. Read on to learn more about each reporter or check out table 1 for...nanobody that would make a useful RANbody, consider leaving a link to its sequence in the comments below. ...29440485. Additional Resources on the Addgene Blog Learn more about using the secondary nanobody toolbox ... plasmids can be found here Watch this video to learn more about nanobodies Additional Resources Looking...

  11. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ....) Fab fragments are created through enzymatic cleavage of the variable region (the antibody region that...to the antigen) from existing antibodies. Each cleaved antibody will produce two ~50kD Fab fragments, ...fragment but created through engineering instead of cleavage. They are comprised of the variable heavy and ...Whatever affinity reagents you use, one thing is clear: it’s an exciting time to be a scientist looking...

  12. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    Published
    Aug. 5, 2021, 12:15 p.m.
    ...This biosensor changes fluorescence based on the release of glutamate from neurons and this new version ...than its predecessors. Currently, Aggarwal, and colleagues Filip Tomaska and Tim Hanson, are characterizing...iGluSnFR3 in vivo and have used it to detect glutamate release from hundreds of synapses at once. “This work has...Aggarwal.In addition to the biosensors, Aggarwal and colleagues are working on developing new surface display...

  13. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...Troubleshooting DNA Sequence Learn Additional Ways to Verify Your Plasmid Learn How Addgene Uses Barcodes.... All of our incoming plasmids are sequenced at least twice, and sometimes more depending on the number...read. You can expect to recover around 750 bp of clean sequence from a typical reaction, or about 250 amino...

  14. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    ...on the symposium as a whole and for registration please go to http://futureofresearch.org/boston/. The ... data and the Labor Market. On this topic it is clear that many important questions, areas for informed...biomedical_research_wgreport.pdf Resources at Addgene Learn More about Careers in Science in Our Career Posts...Meetings from Addgene Executive Director Joanne Kamens Learn how to be a successful manager with our Management...

  15. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ... "traditional" restriction enzymes in that they cleave outside of their recognition sequence, creating... BbsI), that are located on the outside of the cleavage site. As a result, these sites are eliminated ...a few advantages over other cloning methods. Exonuclease-based methods like Gibson assembly require 20...multiple DNA fragments to create TAL effector nucleases. These plasmids were designed to utilize the BsaI...

  16. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    Published
    Aug. 20, 2019, 12:31 p.m.
    ...spectrum. The experimental apparatus should be cleaned thoroughly between animals as olfactory stimuli...animal (ex: urine) can be distracting. If it is not cleaned, animals tested towards the end of an experiment...coordination to more complex situations such as learning and memory or emotional states, for example anxiety...Guide to Viral Vectors Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...

  17. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    Published
    May 24, 2018, 1:20 p.m.
    ...Arabidopsis in Education Learning Synthetic Biology with BioBuilder 3D Models for learning about CRISPR Resources... subsequently destroyed by Argonaute-catalyzed nuclease activity or their translation disrupted through...protocols and want to share pictures, feedback etc. please write to [email protected] and if you make...

  18. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    Published
    May 22, 2018, 1:38 p.m.
    ...Addgene Blog Learn about Synthetic Biology with BioBuilder Use Addgene Videos to Learn about Basic Lab...genes in other species, but these studies generally leave questions about gene function wide open. What if...Check Out Our Molecular Biology Reference Pages Learn Simple Lab Techniques in Our Protocol Videos ...

  19. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    Published
    May 7, 2020, 1:15 p.m.
    ...the promoter region of gamma hemoglobin (HGB1/2) leads to continuous expression of fetal hemoglobin even...efficiency, respectively) and outperformed ABE7.10 by at least 1.4 fold. The ability of ABE8.20-m to edit primary...editing of A•T to G•C in genomic DNA without DNA cleavage. Nature 551:464–471 . https://doi.org/10.1038/...Addgene blog Find more blog posts about base editing Learn about another evolved base editor: ABE8e Read about...

  20. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...adenovirus, and adeno-associated virus (AAV). The least risky of these four viruses is AAV – it is not known...propensity to integrate into proto-oncogenes, which can lead to cancer. These viruses need to be handled in a...thoroughly decontaminated after use, typically with bleach (plastic) or ethanol (other surfaces). For AAVs...and isopropyl alcohol, 0.5% peracetic acid, 10% bleach, or 1% iodine are useful. Some organizations or...
Showing: 401 - 420 of 849 results