We narrowed to 461 results for: MEA;
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TypeBlog PostPublishedFeb. 27, 2018, 2:04 p.m....also too large to be expressed in bacteria, which means their production requires animal sacrifice and precludes...
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Transferable Skills Guide: Identifying Your Transferable Skills
TypeBlog PostPublishedJan. 11, 2018, 3:29 p.m.... you know how to do the job. You may think this means you’re guaranteed to get the job, however, many ... -
MXS Chaining
TypeBlog PostPublishedFeb. 7, 2017, 3:30 p.m....MXS-chaining also has the advantage of being directional, meaning that (in the previous example) one can control ... -
Plasmids 101: Choosing an Antibiotic Resistance Gene
TypeBlog PostPublishedOct. 10, 2023, 1 p.m....? Zeocin is genotoxic when not 100% inhibited, meaning it will induce mutations in the host/plasmid DNA... -
An Introduction to Adenovirus
TypeBlog PostPublishedOct. 3, 2023, 1:15 p.m....synchronized exposure under these conditions once the measures were lifted (Servellita et al., 2023). This anecdote... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog PostPublishedJuly 27, 2023, 1:15 p.m....worthwhile to generalize the idea of “transduction” to mean any transfer of material from the virus to the host... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog PostPublishedNov. 29, 2022, 2:15 p.m....essentialist links between biology and ancestry group as a means of perpetuating racial bias. It is easy, even unintentionally... -
Simplify Cloning with in vivo Assembly
TypeBlog PostPublishedOct. 18, 2018, 12:37 p.m....In our experience, good amplification after PCR means successful plasmid assembly (if primer design is... -
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries
TypeBlog PostPublishedOct. 4, 2018, 12:44 p.m....CRISPRko results in a complete loss of function meaning that if an essential gene plays a role in the desired... -
Lentiviral Vector Uses and Overview
TypeBlog PostPublishedMay 19, 2016, 2:30 p.m....C, Zolopa A, Binder-Scholl G, Tebas P, June CH, Humeau LM, Rebello T. J Gene Med. 2013 Feb;15(2):78-82... -
Plasmids 101: Colony PCR
TypeBlog PostPublishedMay 12, 2016, 2:30 p.m....because you get the expected sized PCR product doesn’t mean there aren’t mutations in your insert. Make sure... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog PostPublishedOct. 6, 2015, 2:30 p.m....tubes. 1D barcodes are better for tubes that are meant to be read one at a time. 1D barcodes can be purchased... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog PostPublishedSept. 3, 2015, 2:30 p.m....outside of our break room. A ring of the chimes means something fun is going on and to get there fast!... -
A Career in Grants: How To Become a Grant Professional
TypeBlog PostPublishedAug. 20, 2015, 2:30 p.m....to help you enter and to have a successful and meaningful career in the grants profession: 1. Build on ... -
Plasmids 101: Protein tags
TypeBlog PostPublishedDec. 11, 2014, 4:26 p.m.... a commercially available antibody, that doesn’t mean it’s a good one), then your first step towards detecting... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog PostPublishedDec. 14, 2017, 2:08 p.m....with Sendai virus requires stringent biosafety measures, thereby increasing the costs of the procedure... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog PostPublishedJuly 23, 2019, 12:46 p.m....transduction was not detected. They also used an ELISA to measure binding of PHP.B to a soluble version of LY6A. ... -
CRISPR-mediated Plant Base Editors
TypeBlog PostPublishedJan. 3, 2019, 1:35 p.m.... from the base (C/A) to be edited. But does this mean a target C or A much further from the ‘NGG’ PAM ... -
Finding nucleic acids with SHERLOCK and DETECTR
TypeBlog PostPublishedApril 16, 2020, 1 p.m....The detectable signal from this reporter can be measured and quantified to determine the presence and quantity... -
Plasmids 101: Plasmid Incompatibility
TypeBlog PostPublishedMarch 31, 2020, 1:15 p.m....that plasmids can be symmetrically incompatible, meaning that both plasmids are lost from the cell with ...