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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 711 results for: Met

Showing: 401 - 420 of 711 results

  1. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...animal sacrifice. Could there be an alternative method for immunodetection? Enter the Görlich lab and ...and extracted with common protein purification methods. Read on to learn more about nanobodies and how...isolating HCab antibodies from its serum, and meticulously screening, optimizing, and characterizing several...

  2. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...Molecular cloning requires some method of screening colonies for the presence of an insert. Traditionally...mini-prep the positive ones. Regardless of which method you choose, make sure to use the appropriate antibiotic...amplify a product, then you know there could be something wrong with the PCR setup and/or the primer design...

  3. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...and its host response. Nat Methods 13:868–874 . https://doi.org/10.1038/nmeth.3993  Cong L, Ran FA, Cox...next-generation sequencing). Using this sensitive method, they found that SaCas9 did not display higher ...

  4. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...Egr3, and Arc. Thus, CRISPRa represents a powerful method to dissect the individual and shared contributions...surface protein for which there are good flow cytometric antibodies, downstream of the TUJ1 coding sequence...these cell types are used. The lack of good flow cytometric antibodies for many CNS-resident cell types (...

  5. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...support entry of required metadata, with the option of adding additional metadata in the repository after...management apps, colony management software, and method repositories like protocols.io. These tools are...

  6. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ... selection Another option is to use a different method of selection entirely. Auxotrophic selection relies...vectors typically involves shuttle vectors, also sometimes called transfer vectors. The gene of interest ...vectors themselves to be a distinct gene delivery method. We focused this post on shuttle vectors delivered...

  7. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...have an enzyme to remove inosine from DNA: N-methylpurine DNA glycosylase (MPG), also known as alkyladenine... DNA, creating an abasic site. MPG / AAG N-methylpurine DNA glycosylase / alkyladenine DNA glycosylase...editing by fusing an adenine base editor with an N-methylpurine DNA glycosylase. Nature Biotechnology, 41(8)...

  8. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...higher than traditional homologous recombination methods). By using mCherry for selection of sgRNA plasmid...Hung, D. (2024). A dual-plasmid CRISPR/Cas9-based method for rapid and efficient genetic disruption in Mycobacterium...kDa) and are compatible with common microscopy methods. Plus, the luminescence wavelength (460 nm) is ...

  9. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...understand the purpose of, or a different way of doing something then you’ve done previously) you can bring those...especially when you are in a new place, learning something new. So how can you remember everything? Write...done a technique before, but you haven’t, say something. It can be intimidating to ask for clarification...

  10. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...story. Viral vectors are, after all, a delivery method. I would be remiss to talk about them without looking...for Adeno-associated viral vector production. Neuromethods, 195, 77–101. https://doi.org/10.1007/978-1-...2019). Adeno-Associated Virus Technologies and Methods for Targeted Neuronal Manipulation. Frontiers in...

  11. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...contribute to this regulation in different ways: methylating newly synthesized DNA, blocking binding sites...copy number and compensating for the additional metabolic cost imposed on the host with a beneficial function.... On the other hand, they also impose a lower metabolic cost on cells and produce lower amounts of protein...

  12. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...a) Plasmid diagram of pBAD-bARGSer-AxeTxe. (b) Methods to assess bARGSer expression, from left to right...produce GVs in their own lab using the most reliable method, which is the transient cotransfection of the mARG...diagram of mARGAna. (b) Schematic of the general method to create your own mARGAna cell line. (c) Z-projected...

  13. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ... assumptions, use of inappropriate statistical methods, omission of some data, etc.  The firestorm that... for increasing both data accessibility and methodological detail. Publishers have also contributed to...to this issue by requiring ever more condensed methods descriptions, legends and limiting numbers of figures...

  14. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...be. If people or teams can’t see eye to eye on something and a disagreement festers, it can lead to hurt...Person A one thing and inadvertently told Person B something else.") The majority of conflicts I address in...communication. Making some assumptions about how something should be communicated is necessary in a large...

  15. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...packaging capacity. Luckily, researchers have devised methods to deliver CRISPR components as ribonucleoproteins...separately from the viral vector. This delivery method frees up space in the AAV allowing a researcher...PMCID: PMC3010411. 2. Kohli, Manu, et al. "Facile methods for generating human somatic cell gene knockouts...

  16. Transferable Skills Guide: Time Management

    Type
    Blog Post
    Published
    Dec. 5, 2017, 1:52 p.m.
    ...respond to friends asking me to dinner saying something like, “I’ll be there in a few minutes, I just ...mind for the endpoints of a task or project (with metrics if possible) and, at the outset of a task, I outline...visit to a university. My to-do list might look something like the image on the right (and for those of ...

  17. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...integrate into the host cell’s genome. However, sometimes inte-great-ion isn’t so great. In this post, we...lentiviral vectors. This is because most titering methods rely on isolating genomic DNA from transduced cells...piece to measure! Instead, two commonly employed methods for titering IDLVs include detecting vector particle...

  18. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...presentation or Ted Talk. You want to give the audience something to think about, argue with, or learn from. Some...comes. 5. Choose interesting content Talk about something you know a lot about and that you care about. ...with your confidence. It is best not to be asked something that you don't know the answer to, but if you ...

  19. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...successfully infected/expressing cells by flow cytometry. The isolated cells were injected into mice and...specificity High sensitivity Easy expression Gives ratiometric data The result was a fusion protein called ...enable sensitive in vivo optical imaging and flow cytometry for monitoring tumorigenesis. Cancer Res. 75, ...

  20. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 2:15 p.m.
    ...serotypes have a primary cellular receptor and sometimes one or multiple coreceptors, which may be present...present on different cell types and sometimes vary between target species or even strains. Pro tip! Getting..., A. C. H., Gokal, D., Feng, Y., Tabar, M. S., Metierre, C., Parsania, C., Song, X., Wang, G., Su, X.-...
Showing: 401 - 420 of 711 results