...contribute to this regulation in different ways: methylating newly synthesized DNA, blocking binding sites...copy number and compensating for the additional metabolic cost imposed on the host with a beneficial function.... On the other hand, they also impose a lower metabolic cost on cells and produce lower amounts of protein...

...understand the purpose of, or a different way of doing something then you’ve done previously) you can bring those...especially when you are in a new place, learning something new. So how can you remember everything? Write...done a technique before, but you haven’t, say something. It can be intimidating to ask for clarification...

...a) Plasmid diagram of pBAD-bARGSer-AxeTxe. (b) Methods to assess bARGSer expression, from left to right...produce GVs in their own lab using the most reliable method, which is the transient cotransfection of the mARG...diagram of mARGAna. (b) Schematic of the general method to create your own mARGAna cell line. (c) Z-projected...

...Egr3, and Arc. Thus, CRISPRa represents a powerful method to dissect the individual and shared contributions...surface protein for which there are good flow cytometric antibodies, downstream of the TUJ1 coding sequence...these cell types are used. The lack of good flow cytometric antibodies for many CNS-resident cell types (...

...and its host response. Nat Methods 13:868–874 . https://doi.org/10.1038/nmeth.3993  Cong L, Ran FA, Cox...next-generation sequencing). Using this sensitive method, they found that SaCas9 did not display higher ...

...support entry of required metadata, with the option of adding additional metadata in the repository after...management apps, colony management software, and method repositories like protocols.io. These tools are...

... assumptions, use of inappropriate statistical methods, omission of some data, etc.  The firestorm that... for increasing both data accessibility and methodological detail. Publishers have also contributed to...to this issue by requiring ever more condensed methods descriptions, legends and limiting numbers of figures...

...integrate into the host cell’s genome. However, sometimes inte-great-ion isn’t so great. In this post, we...lentiviral vectors. This is because most titering methods rely on isolating genomic DNA from transduced cells...piece to measure! Instead, two commonly employed methods for titering IDLVs include detecting vector particle...

...packaging capacity. Luckily, researchers have devised methods to deliver CRISPR components as ribonucleoproteins...separately from the viral vector. This delivery method frees up space in the AAV allowing a researcher...PMCID: PMC3010411. 2. Kohli, Manu, et al. "Facile methods for generating human somatic cell gene knockouts...

...respond to friends asking me to dinner saying something like, “I’ll be there in a few minutes, I just ...mind for the endpoints of a task or project (with metrics if possible) and, at the outset of a task, I outline...visit to a university. My to-do list might look something like the image on the right (and for those of ...

...be. If people or teams can’t see eye to eye on something and a disagreement festers, it can lead to hurt...Person A one thing and inadvertently told Person B something else.") The majority of conflicts I address in...communication. Making some assumptions about how something should be communicated is necessary in a large...

...presentation or Ted Talk. You want to give the audience something to think about, argue with, or learn from. Some...comes. 5. Choose interesting content Talk about something you know a lot about and that you care about. ...with your confidence. It is best not to be asked something that you don't know the answer to, but if you ...

... strong early gene expression, but can become methylated over time, resulting in decreased expression ...Engineered AAV Capsids Improve Transduction and Volumetric Spread in the CNS. Molecular Therapy - Nucleic...hydroxylase promoter in vivo. Molecular Therapy - Methods & Clinical Development, 3, 16062. https://doi.org...

...formalin-fixed, paraffin-embedded tissue sections, methanol- or paraformaldehyde-fixed cell samples, or other...multiplexed over 65 targets!) IF is a versatile method with endless possibilities, and there are always...Multiplexing Immunohistochemistry. Materials and Methods, 9. https://doi.org/10.13070/mm.en.9.2846 Radtke...

...successfully infected/expressing cells by flow cytometry. The isolated cells were injected into mice and...specificity High sensitivity Easy expression Gives ratiometric data The result was a fusion protein called ...enable sensitive in vivo optical imaging and flow cytometry for monitoring tumorigenesis. Cancer Res. 75, ...

... the right lab such a big deal? It’s actually something you CAN choose and it will make a huge difference... help identify your priorities and needs, but sometimes it just takes introspective thought on what’s ...such as the Future of Research Symposium and #MeTooSTEM, academic bullies are hard to stop—they often ...

...Crystal Violet Pre and Post Visible Light 100 Methylene Blue Post Visible Light 100 Ethidium Bromide...from the gel via your favorite gel purification method, it is important to determine the concentration...calculate this based on DNA concentration alone. One method is to conduct 2 ligations for each plasmid you ...

...and volumes to determine the optimal injection parameters needed to transduce your cells of interest. Using...Once you have determined the optimal injection parameters for the serotype that best transduces your cells...performance in your system. It is very rare for a methods section to include all of the variables that need...

... Karl Deisseroth, combined optical and genetic methods to photo-stimulate mammalian neurons and alter ... using pharmacological or electrophysiological methods (Deisseroth, 2011; Mei and Zhang, 2012; Towne et...PMC2820367. Deisseroth, Karl. "Optogenetics." Nature methods 8.1 (2011): 26. PubMed PMID: 21191368. Deubner,...

... genome engineering parameters via a library-on-library approach." Nature methods 12.9 (2015): 823-826...genome-wide libraries for CRISPR screening." Nature methods 11.8 (2014): 783-784. PubMed PMID: 25075903. PubMed...