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We narrowed to 850 results for: NTS

Showing: 401 - 420 of 850 results

  1. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ...Stanford University. Sex and gender are critical components of biological research that are often forgotten...discovery. Tackling sex and gender in research Governments, universities, and, increasingly, corporations...researchers consider the sex of their cells when doing experiments—which can lead to failed research. For example...meant that, by the very nature of the way the experiments were performed, they did not see anything unique...of human clinical trials. And, as Janine Clayton points out, late-stage clinical studies that draw from...

  2. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    Published
    Nov. 24, 2015, 3:30 p.m.
    ...From FPs optimized for oxidizing environments to photoconvertible variants, it seems like GFP is always learning... found suitable pairs, they constructed three components: GBPa-VP16 (activation domain); GBPb-GAL4 (DNA-binding...possibility of adapting GFP lines for optogenetics experiments in specific cell populations. Listen to Our Podcast...proteins with a modular structure, constructing split variants should be relatively straightforward, but it is...

  3. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ... that allows for the assembly of multiple DNA fragments efficiently and accurately in a “single pot.’ ...quantification of this depletion using imaging-based measurements (Ashley et al., 2021).   Schematic showing...Resources page.  Plasmids for delivering CRISPRi components by a single AAV vector are now available from...motif (PAM). They're used to determine the PAM requirements of Cas enzymes. CRISPR-ARE is a new system from.... Check out the new additions.  Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f, now...

  4. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...papers  You’ll need to start following relevant accounts in order to turn Twitter into a paper-finding-...paper-finding-machine. There are four general types of accounts you can follow: Scientists: Following other scientists...investing the time to read them. Organizations: Accounts for nonprofits, research organizations, and universities...research they support. Monitoring these types of accounts can help you find papers related to a particular...tip Checking the number of retweets, likes, and comments on a tweet about a paper can help you gauge how...

  5. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...sure that what we observe through the microscope represents reality as accurately as possible. This is especially...discover more resources for your genomic tagging experiments. Browse the Allen Institute for Cell Science ...of the gene of interest. To get the molecular components of CRISPR/Cas9 (Cas9 protein and the crRNA-tracrRNA...destabilizes the cell’s outer membrane and allows the components to physically enter the cells so they can go ...easier for you to use genomic tagging in your experiments. Many thanks to our guest bloggers from the ...

  6. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    Published
    Oct. 6, 2020, 1:15 p.m.
    ...rely on fusion proteins while others re-engineer components of Cas systems themselves. SunTag, SAM, and VPR... VPR have all shown significant improvements upon the initial dCas9-VP64 method, so there are multiple...much higher activation, dCas9-VP64 is great for experiments that requires modest gene activation. Synergistic...making it a popular method for gene activation experiments. In cases of multiplex gene regulation (activating...lines (Sajwan et al., 2019). SPH SPH combines components of SAM and SunTag activators, fusing the epitope...

  7. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...companies that produce GMO food plants. When a press piece ran on the detector plants, we expected to see that...Facebook comment.  In our day job, we worked on plants engineered to detect explosives and rapidly indicate...that same anger. Instead, social media comments were more often along the lines of “I hate GMOs, but what...entrepreneurship, share your thoughts with us in the comments below. We’re happy to answer questions about this...

  8. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...proper basic training prior to conducting mouse experiments. Some of these techniques are what we call “beginner...as this method is useful for analyzing localized events. Intradermal injections require a bit of extra ...used for delivery of viral vectors, though drug treatments are also possible. One additional requirement...through a feeding tube with a bulb on the end, which prevents accidental tears or punctures to the throat. The...overseeing your work. Good luck with your in vivo experiments! Table 1: Summary of common injection routes ...

  9. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ... 2024 under CC-BY license. They did more experiments to see if any small molecules act as a cofactor...mutagenesis and screening, the EGFP and mScarlet variants were showing no obvious signs of improved response...deprioritize the project, Maria’s screens of AsLOV2 variants showed some significant gains! After a few more... Andrew and Maria expect that further-improved variants of MagLOV will be possible with other engineering... Anderson, E. D., Bell, A. M., Tzouanas, C. N., Avants, B. W., & Robinson, J. T. (2019). Magnetic Entropy...

  10. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...also find anti-Myc [9E10] Fab fragments and single-chain variable fragments.  Abi, on the other hand...Who first started using it in protein tagging experiments? Why Myc? When did the commonly used anti-c-Myc...allow users to better troubleshoot and design experiments. It turns out that even with the most popular...Munro S, Pelham HRB (1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell 48:899–...

  11. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...they have fewer hydrophilic surfaces, require detergents or lipids to remain folded, and are often less... Generation Toolbox and commercially available reagents in three major steps - all without the need to...candidates are then reverse transcribed into cDNA fragments that are efficiently subcloned into the phagemid...research, this protocol holds promise for developing treatments for other diseases or for studying other membrane-bound... Cloning System for Characterization of Enzyme Variants. In: Methods in Molecular Biology. Humana Press...

  12. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    Published
    July 13, 2017, 2:30 p.m.
    ...method for maintaining stable lines of genetic mutants, including in situations where maintaining heterozygosity...function, while others offer fly stocks and other reagents. If you’re ever confused about the best way to...tremendous resource for the fly community. Drosophila reagents are generously shared among the research community...Bloomington, but you can search for various fly reagents on the Bloomington website as well. Additionally...Functional Genomics Resources website has in vitro reagents and tools to help you knock down, knock out, and...

  13. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ...therapeutic agents. Nevertheless, a glimmer of light remains for this elusive target. Recent improvements in computer... circulatory and lymph systems are not ideal environments for many cell types. From proteins to pathways...Platform (Life Technologies, Carlsbad, CA) that represents 180 genes from the Ras signaling pathway. Its...resource! Please leave us your thoughts in the comments section below. Michael G. Lemieux is a Scientist...

  14. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...doesn’t get a lot of press. However, this process accounts for a percentage of the mutations seen with TALENs...even if a repair template is present. HDR also presents a cloning dilemma - the longer the homology, the...explored. More broadly, Sakuma et al.’s use of MMEJ represents another strategy researchers can exploit for ...In organisms where HDR is downregulated, MMEJ represents another method for making targeted modifications...CRISPR Guide Pages Find CRISPR Plasmids for Your Experiments Deposit Your New Plasmid Tools with Us  ...

  15. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    Published
    Dec. 12, 2013, 3:35 p.m.
    ...bacterial transcription and translation initiation elements. Open science with BioBricks The BioBrick Foundation's...Integrase Logic (BIL) gates collection of parts implements the basic logical functions, which we know and...out of 15 are free. Now, the BioBricks Foundation wants to develop a language for programming life. We can... prokaryotic bacteria, not in fungal systems or plants. We are really plugging into the "stuff sector"... collection of reusable, off-the-shelf genetic elements that allow you to begin to imagine designing 10...

  16. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ... may be interested in using them in your own experiments. It is possible to produce your own recombinant... coli is often used for the production of Fab fragments where variable chains are secreted into the periplasmic... step, you’ll want to ensure that your buffer components are compatible with your conjugate and conjugation... options to choose from depending on the time, reagents, and equipment available to you. Whatever you ...Goldstein J. Comparison of predicted extinction coefficients of monoclonal antibodies with experimental values...

  17. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...accessible via web searches; likewise, most in-person events and venues will have a solid understanding of their...both!) will be a major limiting factor at multiple points. Before you embark on a project, do your best to... design a hands-on interactive activity using elephants than it is to design one demonstrating mitosis... communications below!   Format Resource Requirements Pros Cons Video Medium to high Uses visuals.... Easy access from smartphones with low data requirements.  No visuals. Variety of hosting platforms can...

  18. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...infection and screening by PCR enrich for capsid variants with increased ability to infect the cell type...barrier can be identified by PCR later. The PNS presents a different problem: it’s spread throughout the...applications (mechanistic studies and gene therapy treatments for neurological diseases). The Gradinaru Lab... BP, et al. Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain...research. Visit Our AAV Guide Page for info on AAV components, common uses of AAV, and much more! Find ready-to-use...

  19. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    Published
    Aug. 9, 2016, 2:30 p.m.
    ... Quilt CTO Aneesh Karve, shows how to design experiments that work anywhere in the genome. Aneesh's research...the genome” to compute coordinates for genetic elements like protein, RNA, and DNA (Table 1). As with ...transform raw genomic coordinates into meaningful experiments. Table 1: An emerging family of "GPS for the ...of dark matter: enhancers, lncRNAs, repetitive elements, repressors, insulators, and more. We know that...Bedtools getFasta. These regions and their reverse complements were parsed for spCas9 PAM sites (NGG) and then...

  20. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...Once a sample enters Addgene’s lab, a series of events is triggered. Each step is tracked through barcoded...journey begins First, each package is opened and the contents are carefully inspected to verify that the samples... some have been transported across oceans and continents. Each deposit may consist of anywhere from one...appropriate location. The barcode also identifies the contents of each individual tube.  If a plate of samples...people or even multiple labs. Careful tracking prevents us Addgenies from wasting hours of valuable time...
Showing: 401 - 420 of 850 results