We narrowed to 479 results for: RET

1. Experimenting with New Careers while in Grad School

   Type

   Blog Post

   Published

   Feb. 25, 2016, 3:30 p.m.

   ...a lot of attention on social media. I gained a concrete example to use to promote future attempts and ...

2. PITChing MMEJ as an Alternative Route for Gene Editing

   Type

   Blog Post

   Published

   Feb. 23, 2016, 3:30 p.m.

   ... the region between the microhomology and the retention of a single microhomology sequence. For more mechanistic...

3. CRISPR 101: Mammalian Expression Systems and Delivery Methods

   Type

   Blog Post

   Published

   Sept. 24, 2015, 2:30 p.m.

   ...easy Very hard Viral delivery   Lentivirus Retrovirus AAV Cells Dividing or nondividing Dividing ...

4. Genome-wide Screening Using CRISPR

   Type

   Blog Post

   Published

   Aug. 18, 2015, 2:30 p.m.

   ... to skewed results. Selecting a cell type - Theoretically, any cell type can be used in a CRISPR screen...

5. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

   Type

   Blog Post

   Published

   June 12, 2014, 3:30 p.m.

   ...recently to explore their use in plants, including FRET tools, double dimerization GFPs, and circular permutation...

6. Tips for Using BLAST to Verify Plasmids

   Type

   Blog Post

   Published

   May 29, 2014, 1:29 p.m.

   ...This option is not as fast as megablast, but can return longer alignments to compare with your sequencing...

7. DNA Purification Without a Kit

   Type

   Blog Post

   Published

   Feb. 11, 2020, 2:15 p.m.

   ...but trace contamination could interfere with interpreting the results of transfection assays. Go forth...

8. SciComm: Taking Science to Elementary and Middle Schools

   Type

   Blog Post

   Published

   July 9, 2019, 12:52 p.m.

   ... itself in terms of what they do with their discretionary money. They also usually have a good event-planning...

9. Isolating Genomic Regions of Interest with the CRISPR System

   Type

   Blog Post

   Published

   May 30, 2019, 12:44 p.m.

   ...Fujii Lab generated a transgenic mouse line and retroviral expression system for using this approach in ...

10. An Introduction to Adenovirus

    Type

    Blog Post

    Published

    Oct. 3, 2023, 1:15 p.m.

    ...kilobases; compared to only 4.7 kb for AAV, 9 kb for retroviruses). The tradeoff is that adenoviral vector expression...

11. Course-Based Undergraduate Research Experiences (CUREs)

    Type

    Blog Post

    Published

    Feb. 9, 2023, 2:15 p.m.

    ...Zuckerman, 1977; Ma et al., 2020). Many have interpreted this trend as evidence that the best teachers...

12. Uncovering Ancestral Bias in CRISPR Research

    Type

    Blog Post

    Published

    Nov. 29, 2022, 2:15 p.m.

    ...and consider bias as a confounding factor when interpreting findings, particularly when researching ancestry...

13. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type

    Blog Post

    Published

    Sept. 28, 2017, 2:01 p.m.

    ...Multi-color labeling of neurons is for more than making pretty pictures (see figure 4). It also enables single-cell...

14. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type

    Blog Post

    Published

    Sept. 8, 2016, 2:30 p.m.

    ... try out the JF dyes. Acknowledgements I thank Brett Mensh, a wordsmith soldier of fortune, for helpful...

15. Lentiviral Vector Uses and Overview

    Type

    Blog Post

    Published

    May 19, 2016, 2:30 p.m.

    ...researchers developed viral vector systems based on retroviruses like Moloney murine leukemia virus (MMLV). The...

16. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type

    Blog Post

    Published

    March 17, 2016, 2:30 p.m.

    ...independently of other viral proteins simplifying the interpretation of results. This system can be used to develop...

17. Cpf1: A New Tool for CRISPR Genome Editing

    Type

    Blog Post

    Published

    Oct. 14, 2015, 2:30 p.m.

    ...cleaves relatively far away from the PAM, NHEJ might retain the PAM site. Therefore, if HDR does not initially...

18. Negotiating Work and Life: How to Find the Joy

    Type

    Blog Post

    Published

    March 20, 2014, 3:04 p.m.

    ...running errands. Give help when you have time, and in return you will get help when you need it. On the work...

19. New CRISPR Tools: Cas7-11 and PASTE

    Type

    Blog Post

    Published

    Feb. 17, 2022, 2 p.m.

    ...evidence of growth inhibition when the system was retargeted. This finding was surprising – in the most common...

20. An Inside Look into Addgene's Dev Team

    Type

    Blog Post

    Published

    Jan. 23, 2020, 2:45 p.m.

    ...software from Python 2.7 to Python 3.5. Python 2.7 is retiring this year, which means there will be no further...