We narrowed to 488 results for: RET

1. 3D Printing Meets CRISPR Cas9

   Type

   Blog Post

   Published

   Oct. 17, 2017, 12:55 p.m.

   ... is docked on the membrane of the endoplasmic reticulum. And graduate students, momentarily forgetting...

2. Fluorescent Tagging of Endogenous Genes with SapTrap

   Type

   Blog Post

   Published

   Oct. 12, 2017, 2:26 p.m.

   ...of normal protein function, and ultimately misinterpretation of the protein’s cellular role. One way to...

3. Google Forums Round Up: First Impressions of NgAgo

   Type

   Blog Post

   Published

   Aug. 4, 2016, 2:30 p.m.

   ...NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN RETRACTED AND FOLLOW UP STUDIES HAVE FAILED TO DEMONSTRATE...

4. Plasmids 101: Optimizing Plasmid Yields

   Type

   Blog Post

   Published

   May 26, 2016, 2:30 p.m.

   ... in a protein expression strain, we suggest retransforming the plasmid into a strain meant for DNA purification...

5. Viral Vectors 101: Producing Your rAAV

   Type

   Blog Post

   Published

   July 16, 2024, 1:15 p.m.

   ...Golde, T. E. (2020). Utilizing minimally purified secreted rAAV for rapid and cost-effective manipulation...

6. Experimenting with New Careers while in Grad School

   Type

   Blog Post

   Published

   Feb. 25, 2016, 3:30 p.m.

   ...a lot of attention on social media. I gained a concrete example to use to promote future attempts and ...

7. PITChing MMEJ as an Alternative Route for Gene Editing

   Type

   Blog Post

   Published

   Feb. 23, 2016, 3:30 p.m.

   ... the region between the microhomology and the retention of a single microhomology sequence. For more mechanistic...

8. CRISPR 101: Mammalian Expression Systems and Delivery Methods

   Type

   Blog Post

   Published

   Sept. 24, 2015, 2:30 p.m.

   ...easy Very hard Viral delivery   Lentivirus Retrovirus AAV Cells Dividing or nondividing Dividing ...

9. Genome-wide Screening Using CRISPR

   Type

   Blog Post

   Published

   Aug. 18, 2015, 2:30 p.m.

   ... to skewed results. Selecting a cell type - Theoretically, any cell type can be used in a CRISPR screen...

10. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type

    Blog Post

    Published

    June 12, 2014, 3:30 p.m.

    ...recently to explore their use in plants, including FRET tools, double dimerization GFPs, and circular permutation...

11. Tips for Using BLAST to Verify Plasmids

    Type

    Blog Post

    Published

    May 29, 2014, 1:29 p.m.

    ...This option is not as fast as megablast, but can return longer alignments to compare with your sequencing...

12. SciComm: Taking Science to Elementary and Middle Schools

    Type

    Blog Post

    Published

    July 9, 2019, 12:52 p.m.

    ... itself in terms of what they do with their discretionary money. They also usually have a good event-planning...

13. Isolating Genomic Regions of Interest with the CRISPR System

    Type

    Blog Post

    Published

    May 30, 2019, 12:44 p.m.

    ...Fujii Lab generated a transgenic mouse line and retroviral expression system for using this approach in ...

14. Uncovering Ancestral Bias in CRISPR Research

    Type

    Blog Post

    Published

    Nov. 29, 2022, 2:15 p.m.

    ...and consider bias as a confounding factor when interpreting findings, particularly when researching ancestry...

15. An Introduction to Adenovirus

    Type

    Blog Post

    Published

    Oct. 3, 2023, 1:15 p.m.

    ...kilobases; compared to only 4.7 kb for AAV, 9 kb for retroviruses). The tradeoff is that adenoviral vector expression...

16. Course-Based Undergraduate Research Experiences (CUREs)

    Type

    Blog Post

    Published

    Feb. 9, 2023, 2:15 p.m.

    ...Zuckerman, 1977; Ma et al., 2020). Many have interpreted this trend as evidence that the best teachers...

17. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type

    Blog Post

    Published

    Sept. 28, 2017, 2:01 p.m.

    ...Multi-color labeling of neurons is for more than making pretty pictures (see figure 4). It also enables single-cell...

18. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type

    Blog Post

    Published

    Sept. 8, 2016, 2:30 p.m.

    ... try out the JF dyes. Acknowledgements I thank Brett Mensh, a wordsmith soldier of fortune, for helpful...

19. Lentiviral Vector Uses and Overview

    Type

    Blog Post

    Published

    May 19, 2016, 2:30 p.m.

    ...researchers developed viral vector systems based on retroviruses like Moloney murine leukemia virus (MMLV). The...

20. DNA Purification Without a Kit

    Type

    Blog Post

    Published

    Feb. 11, 2020, 2:15 p.m.

    ...but trace contamination could interfere with interpreting the results of transfection assays. Go forth...