We narrowed to 464 results for: SHI
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TypeBlog PostPublishedDec. 1, 2015, 3:30 p.m....they are making fairly remarkable progress on establishing a system for the chemical replication of RNA...
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Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog PostPublishedJuly 23, 2015, 2:30 p.m..... Since makerspaces are generally funded on a membership model - everyone contributes each month - with... -
How-to: 5 Steps to a Great Panel Discussion
TypeBlog PostPublishedOct. 22, 2014, 1:57 p.m....attention of the audience – phones come out, postures shift, they are checking out. If you can, plant 1-2 good... -
10 Basic tips for mammalian cell culture
TypeBlog PostPublishedJuly 12, 2018, 1:09 p.m.... incubator. This also means that any medium or washing buffer, such as PBS (Phosphate Buffered Saline)... -
3D Printing Meets CRISPR Cas9
TypeBlog PostPublishedOct. 17, 2017, 12:55 p.m....potential of this protein to undergo conformational shifts. This backbone model of Cas9 is based on Fuguo ... -
Common Injection Routes in Mice
TypeBlog PostPublishedJan. 22, 2025, 9:42 p.m....131–141. https://doi.org/10.4236/jbm.2020.89011 Shimizu, S. S. (2004). Routes of administration. In H. ... -
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs
TypeBlog PostPublishedJune 25, 2024, 1:15 p.m....any prime editor experimentalist, and especially shines once large-scale prime editing experiments come... -
Antibodies 101: Conventional vs Spectral Flow Cytometry
TypeBlog PostPublishedAug. 3, 2023, 1:15 p.m....emerged as a particularly useful tool for those wishing to run larger panels: five laser spectral flow ... -
Anatomy of a Plasmid Page at Addgene
TypeBlog PostPublishedFeb. 4, 2016, 3:30 p.m....grow the bacterial strain in which the plasmid is shipped. Copy number (if known), antibiotic resistance,... -
Light Sheet Fluorescence Microscopy
TypeBlog PostPublishedJuly 16, 2015, 2:30 p.m.... our understanding of the structure-function relationship of the central nervous system under normal and... -
Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments
TypeBlog PostPublishedSept. 23, 2014, 3:05 p.m....online protocol repository hosted by the Nature Publishing Group. Like the previously mentioned sites, NPE... -
Tips for Using BLAST to Verify Plasmids
TypeBlog PostPublishedMay 29, 2014, 1:29 p.m....against the database. Another benefit is that a frameshift mutation present in the ORF is readily apparent... -
Tips for arabidopsis transformation
TypeBlog PostPublishedOct. 25, 2018, 1:23 p.m....are a couple of my favorites. Plasmids from the Tsuyoshi Nakagawa lab are binary gateway vectors and can... -
SciComm: Taking Science to Elementary and Middle Schools
TypeBlog PostPublishedJuly 9, 2019, 12:52 p.m....them had imagined for a scientist. I think that establishing this history helped demonstrate that a) I was... -
Plasmids 101: Choosing an Antibiotic Resistance Gene
TypeBlog PostPublishedOct. 10, 2023, 1 p.m....their mode of action and have a very similar relationship as amp and carb (they are interchangeable). ... -
Getting to Know Expansion Microscopy
TypeBlog PostPublishedJune 20, 2023, 1:15 p.m....they want to understand the ultrastructural relationships that influence everything from cell division... -
CRISPR 101: Making a Knock-In Cell Line
TypeBlog PostPublishedDec. 22, 2022, 2:15 p.m....is to disrupt DNA translation by generating a frameshift mutation. The majority of cellular repair events... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog PostPublishedAug. 27, 2020, 1:15 p.m....levels. BiFC quantification This is where BiFC outshines FRET — BiFC experiments only require that you ... -
A Guide to Starting Your Own Journal Club
TypeBlog PostPublishedFeb. 6, 2020, 3 p.m....blog posts centered around scientific papers and publishing Find tips for leading a great meeting ... -
CRISPR Antimicrobials
TypeBlog PostPublishedMay 3, 2016, 2:30 p.m....phages and antibiotics. Phage therapy fell out of fashion with the introduction of antibiotics, but the field...