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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 459 results for: SHI

Showing: 401 - 420 of 459 results

  1. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ... incubator. This also means that any medium or washing buffer, such as PBS (Phosphate Buffered Saline)...

  3. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...131–141. https://doi.org/10.4236/jbm.2020.89011 Shimizu, S. S. (2004). Routes of administration. In H. ...

  5. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...potential of this protein to undergo conformational shifts. This backbone model of Cas9 is based on Fuguo ...

  6. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...grow the bacterial strain in which the plasmid is shipped. Copy number (if known), antibiotic resistance,...

  7. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ... our understanding of the structure-function relationship of the central nervous system under normal and...

  8. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...against the database. Another benefit is that a frameshift mutation present in the ORF is readily apparent...

  10. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...are a couple of my favorites. Plasmids from the Tsuyoshi Nakagawa lab are binary gateway vectors and can...

  14. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...they want to understand the ultrastructural relationships that influence everything from cell division...

  15. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...is to disrupt DNA translation by generating a frameshift mutation. The majority of cellular repair events...

  16. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...phages and antibiotics. Phage therapy fell out of fashion with the introduction of antibiotics, but the field...

  17. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...upstream of the PAM site, Cpf1 cleaves in a staggered fashion, creating a 5 nucleotide 5’ overhang 18-23 bases...
Showing: 401 - 420 of 459 results